Media | Number of tested hermaphrodites | Lifespan (days) Mean ± SE | Log-rank |
---|---|---|---|
NGM | 107 | 14.96 ± 0.42 | Control |
NcM6 | 71 | 16.35 ± 0.51 | 0.4 |
NcM12 | 76 | 15.49 ± 0.53 | 1.0 |
NcM18 | 133 | 12.3 ± 0.35 | 1.3e−05 |
Number of surviving worms | ||||
---|---|---|---|---|
Day | NGM | NcM6 | NcM12 | NcM18 |
1 | 90 | 96 | 83 | 92 |
2 | 90 | 96 | 83 | 92 |
3 | 90 | 96 | 83 | 92 |
4 | 90 | 96 | 83 | 92 |
5 | 86 | 96 | 83 | 92 |
6 | 86 | 96 | 83 | 92 |
7 | 86 | 95 | 83 | 92 |
8 | 85 | 95 | 77 | 87 |
9 | 85 | 93 | 73 | 86 |
10 | 85 | 93 | 69 | 86 |
11 | 81 | 93 | 68 | 85 |
12 | 64 | 91 | 60 | 83 |
13 | 46 | 91 | 60 | 83 |
14 | 29 | 91 | 60 | 74 |
15 | 15 | 81 | 59 | 69 |
16 | 6 | 71 | 58 | 62 |
17 | 3 | 64 | 58 | 60 |
18 | 0 | 59 | 56 | 51 |
19 | 51 | 51 | 36 | |
20 | 41 | 50 | 25 | |
21 | 28 | 47 | 17 | |
22 | 8 | 36 | 9 | |
23 | 5 | 15 | 1 | |
24 | 0 | 1 | 0 | |
25 | 0 |
Number of surviving worms | ||||||||
---|---|---|---|---|---|---|---|---|
Day | NGM | NcM6 | NcM8 | NcM9 | NcM12 | NcM18 | NcM20 | NcM25 |
1 | 73 | 70 | 72 | 34 | 66 | 50 | 63 | 57 |
2 | 73 | 70 | 72 | 34 | 66 | 50 | 63 | 57 |
3 | 73 | 70 | 72 | 34 | 66 | 50 | 63 | 57 |
4 | 73 | 70 | 72 | 34 | 66 | 50 | 63 | 57 |
5 | 72 | 69 | 72 | 34 | 66 | 49 | 63 | 57 |
6 | 72 | 69 | 72 | 34 | 65 | 49 | 63 | 57 |
7 | 70 | 69 | 70 | 34 | 64 | 48 | 62 | 55 |
8 | 70 | 69 | 70 | 34 | 64 | 48 | 61 | 54 |
9 | 70 | 68 | 70 | 34 | 62 | 46 | 59 | 52 |
10 | 67 | 68 | 63 | 32 | 61 | 46 | 59 | 52 |
11 | 63 | 68 | 55 | 31 | 60 | 45 | 58 | 51 |
12 | 58 | 62 | 48 | 30 | 57 | 42 | 56 | 51 |
13 | 52 | 55 | 41 | 30 | 54 | 39 | 52 | 51 |
14 | 46 | 42 | 36 | 30 | 49 | 33 | 51 | 50 |
15 | 33 | 23 | 32 | 30 | 37 | 17 | 47 | 49 |
16 | 21 | 12 | 18 | 30 | 26 | 13 | 36 | 39 |
17 | 12 | 5 | 14 | 28 | 15 | 9 | 28 | 38 |
18 | 11 | 4 | 8 | 28 | 11 | 6 | 21 | 33 |
19 | 10 | 3 | 7 | 28 | 7 | 5 | 14 | 30 |
20 | 6 | 2 | 3 | 27 | 3 | 4 | 10 | 28 |
21 | 5 | 1 | 3 | 21 | 0 | 2 | 7 | 17 |
22 | 2 | 0 | 1 | 16 | 1 | 2 | 9 | |
23 | 2 | 1 | 14 | 1 | 1 | 8 | ||
24 | 2 | 1 | 11 | 1 | 0 | 5 | ||
25 | 1 | 1 | 7 | 1 | 0 | |||
26 | 0 | 0 | 4 | 1 | ||||
27 | 3 | 1 | ||||||
28 | 2 | 1 | ||||||
29 | 2 | 0 | ||||||
30 | 1 | |||||||
31 | 0 | |||||||
Censored | 7 | 10 | 8 | 6 | 14 | 5 | 17 | 23 |
Source of cholesterola | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Plant cooking oil | Chicken egg | Pig fat | Mushroom | Soil | |||||||||||||||||||||
Component (Amount/ L) | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM |
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Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | Ѵ | ||
Media type | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM | NcM |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 |
Media | Number of tested hermaphrodites | Total brood size (Mean ± SE) | Dunnett’s post-hoc |
---|---|---|---|
NGM | 39 | 254.7 ± 12.8 | Control |
NcM1 | 57 | 144.9 ± 8.8 | <0.001 |
NcM2 | 50 | 123.8 ± 8.7 | <0.001 |
NcM3 | 55 | 157.5 ± 10.6 | <0.001 |
NcM4 | 55 | 123.8 ± 14.4 | <0.001 |
NcM5 | 25 | 142.9 ± 14.8 | <0.001 |
NcM6 | 42 | 240.5 ± 18.0 | 0.0228 |
NcM7 | 17 | 171.1 ± 18.3 | <0.001 |
NcM8 | 26 | 265.8 ± 8.7 | 0.5468 |
NcM9 | 32 | 194.5 ± 9.1 | <0.001 |
NcM10 | 29 | 152.3 ± 17.3 | <0.001 |
NcM11 | 24 | 163.9 ± 12.5 | <0.001 |
NcM12 | 21 | 234.0 ± 8.8 | 0.1817 |
NcM13 | 50 | 128.9 ± 14.2 | <0.001 |
NcM14 | 47 | 183.6 ± 7.5 | <0.001 |
NcM15 | 43 | 203.6 ± 7.9 | <0.001 |
NcM16 | 40 | 198.6 ± 9.3 | <0.001 |
NcM17 | 38 | 177.2 ± 17.5 | <0.001 |
NcM18 | 44 | 251.7 ± 16.2 | 1.0 |
NcM19 | 38 | 163.2 ± 12.9 | <0.001 |
NcM20 | 35 | 48.7 ± 12.9 | <0.001 |
NcM21 | 26 | 178.9 ± 12.4 | <0.001 |
NcM22 | 35 | 183.4 ± 11.6 | <0.001 |
NcM23 | 16 | 138.4 ± 17.3 | <0.001 |
NcM24 | 39 | 178.7 ± 9.5 | <0.001 |
NcM25 | 45 | 167.0 ± 8.2 | <0.001 |
Media | Number of tested hermaphrodites | Lifespan (days) Mean ± SE | Log-rank |
---|---|---|---|
NGM | 73 | 15.2 ± 0.4 | Control |
NcM6 | 70 | 14.8 ± 0.3 | 1.0 |
NcM8 | 72 | 14.5 ± 0.4 | 1.0 |
NcM9 | 34 | 21.9 ± 0.9 | 4e−09 |
NcM12 | 66 | 15.6 ± 0.4 | 1.0 |
NcM18 | 50 | 15.1 ± 0.5 | 1.0 |
NcM20 | 63 | 16.9 ± 0.5 | 0.39 |
NcM25 | 57 | 18.8 ± 0.6 | 2.4e−05 |
Media | Number of tested hermaphrodites | Total brood size (Mean ± SE) | Dunnett’s post hoc |
---|---|---|---|
NGM | 29 | 248.0 ± 9.7 | Control |
NcM6 | 38 | 200.2 ± 2.9 | <1e−10 |
NcM12 | 39 | 185.6 ± 7.7 | <1e−10 |
NcM18 | 38 | 227.7 ± 7.8 | <1e−10 |
Media | Number of tested hermaphrodites | Lifespan (days) Mean ± SE | Log-rank |
---|---|---|---|
NGM | 90 | 13.41 ± 0.26 | Control |
NcM6 | 96 | 18.97 ± 0.35 | <2e−16 |
NcM12 | 83 | 18.81 ± 0.63 | 9.4e−16 |
NcM18 | 92 | 17.93 ± 0.41 | <2e−16 |
Costa (VND) | |||||
---|---|---|---|---|---|
Components (amount/L) | NcM6 | NcM8 | NcM12 | NcM18 | NGM |
Cholesterol (1 mL of 5 mg/mL) | 200 | ||||
Plant cooking oil (100 µL) | 2 | ||||
Chicken egg (0.56 g) | 56 | 56 | |||
Pig fat (0.4 g) | 28 | ||||
Autoclaved mushroom (20 mL) | 115 | 115 | 115 | 115 | |
Peptone (2.5 g) | 3000 | ||||
Pure mineral mixture (1 mL of 1 M CaCl2, 1 mL of 1 M MgSO4, 25 mL of 1 M KPO4) | 3788 | 3788 | |||
Soil solution (10 mL) | 0 | 0 | 0 | ||
Agar (17 g) | 34,000 | 34,000 | 34,000 | 34,000 | 34,000 |
NaCl (4 mL of 0.75 g/ mL) | 3,000 | 3,000 | 3,000 | 3,000 | 3,000 |
Distilled water with reverse osmosis (1 L) | 2,000 | 2,000 | 2,000 | 2,000 | 2,000 |
Total cost in VND | 39,117 | 42,959 | 39,171 | 39,143 | 45,988 |
Total cost in US$b | 1.70 | 1.87 | 1.70 | 1.70 | 2.00 |
Media | Number of tested hermaphrodites | Total brood size (Mean ± SE) | Dunnett’s post hoc |
---|---|---|---|
NGM | 37 | 23.3 ± 5.0 | Control |
NcM6 | 42 | 54.8 ± 14.6 | <0.001 |
NcM12 | 37 | 48.2 ± 8.4 | <0.001 |
NcM18 | 32 | 67.2 ± 15.7 | <0.001 |
Number of surviving worms | ||||
---|---|---|---|---|
Day | NGM | NcM6 | NcM12 | NcM18 |
1 | 107 | 71 | 76 | 133 |
2 | 107 | 71 | 76 | 133 |
3 | 107 | 71 | 76 | 133 |
4 | 107 | 71 | 76 | 133 |
5 | 107 | 71 | 76 | 133 |
6 | 107 | 71 | 76 | 133 |
7 | 105 | 71 | 76 | 133 |
8 | 103 | 70 | 73 | 112 |
9 | 97 | 68 | 67 | 91 |
10 | 85 | 63 | 62 | 77 |
11 | 81 | 60 | 61 | 64 |
12 | 75 | 57 | 57 | 53 |
13 | 66 | 53 | 52 | 44 |
14 | 56 | 47 | 45 | 30 |
15 | 46 | 42 | 36 | 24 |
16 | 36 | 33 | 30 | 21 |
17 | 27 | 24 | 21 | 20 |
17 | 22 | 20 | 14 | 13 |
19 | 17 | 15 | 12 | 7 |
20 | 13 | 13 | 10 | 5 |
21 | 7 | 10 | 10 | 4 |
22 | 4 | 10 | 4 | 2 |
23 | 3 | 5 | 4 | 2 |
24 | 2 | 2 | 4 | 2 |
25 | 2 | 1 | 3 | 1 |
26 | 2 | 0 | 1 | 1 |
27 | 1 | 1 | 0 | |
28 | 1 | 1 | ||
29 | 1 | 1 | ||
30 | 0 | 0 |
Source | All | NcM6 | NcM8 | NcM9 | NcM12 | NcM18 | NcM20 | NcM25 |
---|---|---|---|---|---|---|---|---|
|
0.342 | 0.879 | 0.67 | 0.0205 | 5.84e−05 | 0.571 | 0.2198 | 0.1003 |
Source | NcM6 | NcM8 | NcM9 | NcM12 | NcM18 | NcM20 | NcM25 | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Comparison |
mean ± SE | NcM6a | NGMb | mean ± SE | NcM8a | NGMb | mean ± SE | NcM9a | NGMb | mean ± SE | NcM12a | NGMb | mean ± SE | NcM18a | NGMb | mean ± SE | NcM20a | NGMb | mean ± SE | NcM25a | NGMb |
NcM6 | 5.8 ± 0.7 | Ref | <0.001 | 10.8 ± 0.7 | 1.0 | 0.6302 | 15.8 ± 1.4 | 0.02654 | 1.0 | 15.8 ± 1.8 | 0.84529 | 0.41764 | 14.1 ± 4.2 | 0.723 | 0.93 | 12.5 ± 3.0 | 0.9786 | <1e−05 | 6.7 ± 1.7 | 1.0 | 0.3859 |
NcM8 | 10.8 ± 3.4 | 0.956 | 0.00641 | 11.7 ± 5.3 | Ref | 0.7559 | 10.0 ± 2.6 | 0.29024 | 0.9642 | 5.8 ± 1.4 | 0.03458 | 1.0 | 9.9 ± 2.1 | 1.0 | 1.0 | 9.2 ± 1.8 | 1.0 | <1e−05 | 22.5 ± 5.6 | 0.0711 | 0.9923 |
NcM9 | 0.8 ± 0.7 | 0.0956 | <0.001 | 3.3 ± 1.2 | 0.39 | 0.324 | 0.0 ± 0.0 | Ref | 0.0571 | 6.7 ± 3.3 | 0.05005 | 1.0 | 13.3 ± 5.0 | 0.845 | 0.98 | 2.5 ± 1.4 | 0.174 | <1e−05 | 6.7 ± 2.4 | 1.0 | 0.3861 |
NcM12 | 9.2 ± 3.0 | 0.997 | 0.00139 | 17.5 ± 3.2 | 0.756 | 1.0 | 22.5 ± 4.5 | <0.001 | 0.4818 | 21.7 ± 3.6 | Ref | 0.05008 | 10.7 ± 1.7 | 0.997 | 1.0 | 11.7 ± 1.9 | 0.9986 | <1e−05 | 15.0 ± 4.8 | 0.5534 | 0.9984 |
NcM18 | 9.2 ± 4.5 | 0.997 | 0.00304 | 8.3 ± 3.4 | 0.984 | 0.2928 | 20.8 ± 4.8 | 0.00216 | 0.7125 | 29.2 ± 7.9 | 0.63337 | 0.00142 | 8.2 ± 1.8 | Ref | 1.0 | 4.2 ± 0.7 | 0.4101 | <1e−05 | 1.7 ± 0.8 | 0.9984 | 0.0938 |
NcM20 | 7.5 ± 3.0 | 1.0 | 0.00300 | 8.3 ± 3.8 | 0.984 | 0.2929 | 8.3 ± 0.8 | 0.48166 | 0.821 | 2.5 ± 1.4 | 0.00731 | 0.97096 | 14.1 ± 2.7 | 0.731 | 0.935 | 10.0 ± 3.1 | Ref | <1e−05 | 13.3 ± 4.6 | 0.7350 | 0.9756 |
NcM25 | 1.7 ± 0.8 | 0.985 | <0.001 | 4.2 ± 1.4 | 0.505 | 0.0491 | 2.5 ± 1.4 | 0.99881 | 0.1598 | 5.0 ± 0.8 | 0.0239 | 0.99996 | 3.3 ± 1.1 | 0.855 | 0.61 | 2.5 ± 1.4 | 0.1741 | <1e−05 | 5.0 ± 2.8 | Ref | 0.2531 |
NGM | 32.5 ± 8.0 | <0.001 | Ref | 17.5 ± 2.5 | 0.756 | Ref | 14.2 ± 6.4 | 0.05703 | Ref | 6.7 ± 2.0 | 0.0499 | Ref | 9.9 ± 1.1 | 1.0 | Ref | 33.3 ± 3.1 | <0.001 | Ref | 18.3 ± 8.0 | 0.2531 | Ref |
Center | 5.8 ± 3.0 | 1.0 | <0.001 | 1.7 ± 1.4 | 0.214 | 0.0137 | 0.8 ± 0.7 | 1.0 | 0.0818 | 1.7 ± 0.8 | 0.00482 | 0.92278 | 6.6 ± 3.5 | 1.0 | 0.982 | 0.8 ± 0.7 | 0.0619 | <1e−05 | 0.0 ± 0.0 | 0.9756 | 0.0536 |