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Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

Phougeishangbam Rolish Singh
Phougeishangbam Rolish Singh
, 
Gerrit Karssen
Gerrit Karssen
, 
Kelvin Gitau
Kelvin Gitau
, 
Cecilia Wanjau
Cecilia Wanjau
, 
Marjolein Couvreur
Marjolein Couvreur
, 
Njira Njira Pili
Njira Njira Pili
, 
Godelieve Gheysen
Godelieve Gheysen
 et 
Wim Bert
Wim Bert
   | 01 janv. 2021
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Publié en ligne: 01 janv. 2021
Pages: 1 - 14
DOI: https://doi.org/10.21307/jofnem-2021-016
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© 2021 Authors, published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Light microscopy and scanning electron microscopy images of Rotylenchus wimbii n. sp. female. A to C: En face view; D to I: Anterior part of the body showing lip and neck region; J: Whole female body; K to N: Vulva region; O to V: Tail region.
Light microscopy and scanning electron microscopy images of Rotylenchus wimbii n. sp. female. A to C: En face view; D to I: Anterior part of the body showing lip and neck region; J: Whole female body; K to N: Vulva region; O to V: Tail region.

Figure 2:

Illustrations of Rotylenchus wimbii n. sp. female. A, B: Anterior part of the body showing lip and neck region; C: En face view; D, E: Lip region; F: Whole body; G to J: Tail region; K: Vulva region. Scales are given in µm.
Illustrations of Rotylenchus wimbii n. sp. female. A, B: Anterior part of the body showing lip and neck region; C: En face view; D, E: Lip region; F: Whole body; G to J: Tail region; K: Vulva region. Scales are given in µm.

Figure 3:

BI phylogenetic tree generated from the analysis of D2-D3 of 28S rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.
BI phylogenetic tree generated from the analysis of D2-D3 of 28S rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

Figure 4:

BI phylogenetic tree generated from the analysis of ITS of rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.
BI phylogenetic tree generated from the analysis of ITS of rDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

Figure 5:

BI phylogenetic tree generated from the analysis of COI of mtDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.
BI phylogenetic tree generated from the analysis of COI of mtDNA sequences using GTR + G + I nucleotide substitution model. Bayesian posterior probabilities are given next to each node and sequences of Rotylenchus wimbii n. sp. are in bold.

Morphometric data for fixed female Rotylenchus wimbii n. sp. specimens mounted in glycerin.

Female character
n 19
Body length (L) 690  ±  44 (620-760)
a = L/MBD 27.3 ± 3.0 (23.0-33.0)
b = L/Anterior end to pharynx-intestine junction 5.8 ± 0.3 (5.0-6.0)
b′ = L/Anterior end to end of pharyngeal gland 5.1 ± 0.4 (4.5-6.1)
c = L/Tail length 66.0 ± 12.0 (49.0-91.0)
c′ = Tail length/ABD 0.6 ± 0.1 (0.3-0.8)
V = Anterior end to vulva opening/L × 100 58 ± 1 (55-60)
Lip region height 4.6 ± 0.3 (4.2-5.3)
Lip region width 8.2 ± 0.3 (7.7-8.7)
Stylet length 25.3 ± 0.9 (22.5-26.6)
Cone length 12.5 ± 0.7 (10.8-13.7)
Shaft length 10.3 ± 0.5 (9.4-11.2)
Stylet knob height 2.6 ± 0.4 (1.9-3.4)
Cone % Stylet 49.3 ± 1.8 (44.8-52.6)
Dorsal gland opening from stylet base 5.4 ± 0.6 (4.1-6.3)
Anterior end to secretory-excretory pore 107 ± 6.2 (93.2-118)
Anterior end to pharynx-intestine junction 118 ± 5.3 (105-128)
Anterior end to pharyngeal gland end 135 ± 6.0 (121-144)
Anterior end to vulva opening 400 ± 24.8 (341-408)
Maximum body diameter (MBD) 25.5 ± 2.9 (21.5-31.7)
Anal body diameter (ABD) 16.9 ± 1.4 (15.2-19.9)
Tail length 10.8 ± 2.1 (6.9-13.4)
Phasmid to tail tip length 31.6 ± 3.1 (25.1-36.3)
Number of annuli between phasmid and tail tip 20 ± 3 (12-26)
Number of tail annuli 7 ± 1 (5-9)

Comparison of some important female characters and information on availability of males of 23 Rotylenchus species reported from Africa.

Species Body length (mm) Lip region Lip annuli number Stylet length (µm) Cone% of stylet Spermatheca in matured females V% Male Tail annuli number Phasmid position
R. abnormecaudatus 0.6-0.7 Broadly rounded, setoff to continuous 4-5 24-25 42-45 Not seen 56-59 Not found 8-11 8-14 annuli anterior to anus
R. acuspicaudatus 0.8-1.0 Broadly rounded, slightly setoff 4-5 26-28 43-51 Rounded, filled with sperms 54-57 Found 13-16 7-15 annuli anterior to anus
R. alius 0.5-0.9 Truncate, blunt anterior, not setoff 4-5 20-31 42-51 Rounded, filled with sperms 54-66 Found 8-15 3-4 annuli posterior to anus
R. bialaebursus 0.6-0.8 Rounded, not setoff 4 26-29 46-50 Rounded, filled with sperms 54-59 Found 6-8 9-12 annuli anterior to anus
R. brevicaudatus 0.5-0.8 Broad, rounded, not setoff 4-5 18-26 – Present 52-62 Found 5-11 8-14 annuli anterior to anus
R. capensis 0.7-1.2 Hemispherical, slightly setoff 5-7 25-35 39-45 Rounded, filled with sperms 54-59 Found 9-14 1-3 annuli anterior to anus
R. catharinae 0.8-1.1 Hemispherical, slightly setoff 7-8 30-33 45-48 Rounded, filled with sperms 55-56 Found 13-14 1-4 annuli anterior to anus
R. caudaphasmidius 0.6-0.9 Hemispherical, not setoff 5 24-32 – Rounded, filled with sperms 55-63 Found 12 5 annuli posterior to anus
R. cypriensis 0.5-0.8 Hemispherical, well setoff 3-4 21-28 – Small and empty 57-67 Not found 6-7 7-14 annuli anterior to anus
R. devonensis 0.7-0.9 Conoid, blunt, not setoff 4 25-34 43-46 Rounded, filled with sperms 53-64 Found 7-9 8-14 annuli anterior to anus
R. wimbii n. sp. 0.6-0.8 Hemispherical, not setoff 4 23-27 45-53 Indistinct to empty 55-60 Not found 5-9 7-17 annuli anterior to anus
R. gracilidens 0.9-1.2 Hemispherical, slightly setoff 4 29-35 – Rounded, filled with sperms 53-59 Found 8-12 15-20 annuli anterior to anus
R. incultus 0.6-1.0 Hemispherical, not setoff 4-5 22-32 – Developed, filled with sperms 53-61 Found ca 8 13-14 annuli anterior to anus
R. karooensis 0.7-1.0 Rounded, slightly setoff 4-5 21-27 43-47 Rounded, filled with sperms 51-60 Found 7-15 1-6 annuli anterior to anus
R. kenti 0.5-0.7 Rounded, not setoff 4-5 21-25 44-52 Rounded, filled with sperms 60-66 Found 9-17 3-4 annuli anterior to anus
R. mabelei 0.9-1.1 Hemispherical, slightly setoff 4-5 27-30 43-50 Empty 48-57 Not found 14-18 3 annuli anterior to 5 annuli posterior of anus
R. minutus 0.5-0.8 Angularly hemispherical, slightly setoff 3-4 20-27 56-58 Rounded, filled with sperms 53-66 Found 9-14 1 annule anterior to 4 annuli posterior of anus
R. mirus 0.6-0.7 Hemispherical, setoff 4-5 20-23 43-50 Rounded, filled with sperms 55-58 Found 6-10 5-12 annuli anterior to anus
R. robustus 0.9-1.6 Hemispherical, well setoff 6-7 33-50 50-56 Rounded, filled with sperms 52-58 Found 8-17 7 annuli anterior to 3 annuli posterior of anus
R. rugatocuticulatus 1.0-1.3 Rounded, slightly setoff 4 35-42 – Developed, filled with sperms 51-56 Found 12 8-10 annuli anterior to anus
R. triannulatus 0.6-0.8 Rounded, well setoff 3 24-25 43-48 Rounded, filled with sperms 56-60 Found 10-11 Anal level to 8 annuli anterior
R. unisexus 0.5-0.9 Hemispherical, not setoff 4 20-29 – Not seen 47-59 Not found 9-13 6-7 annuli anterior to anus
R. usitatus 1.1-1.2 Flattened anteriorly, not setoff 4-5 29-29 43-47 Rounded, filled with sperms 45-55 Found 10-16 8-14 annuli anterior to anus
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