Accès libre

Morphological and molecular characterizations of Heterodera oryzae in Korea

Rose Mwesige
Rose Mwesige
, 
Eun-Hwa Kim
Eun-Hwa Kim
, 
Eun-Hyung Park
Eun-Hyung Park
 et 
Hyoung-Rai Ko
Hyoung-Rai Ko
   | 06 nov. 2020
Journal of Nematology's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Publié en ligne: 06 nov. 2020
Pages: 1 - 12
DOI: https://doi.org/10.21307/jofnem-2020-113
Mots clés
© 2020 Rose Mwesige et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Morphological characteristics of cyst, vulval cone, and second-stage juvenile of Heterodera sp. (SG936). A-C: Cysts, (A) Lemon-shaped cyst (B) Anterior view (C) Underbridge level view, D-H: Second-stage juveniles (J2), (D) Entire J2 body (E) Stylet (F) Lateral field (G) Pharyngeal region (H) Tail region (black arrow: anus). *Scale bars: A = 100 μm, B = 50 μm, C = 50 μm, D = 100 μm, E = 20 μm, F = 10 μm, G = 50 μm, and H = 50 μm.
Morphological characteristics of cyst, vulval cone, and second-stage juvenile of Heterodera sp. (SG936). A-C: Cysts, (A) Lemon-shaped cyst (B) Anterior view (C) Underbridge level view, D-H: Second-stage juveniles (J2), (D) Entire J2 body (E) Stylet (F) Lateral field (G) Pharyngeal region (H) Tail region (black arrow: anus). *Scale bars: A = 100 μm, B = 50 μm, C = 50 μm, D = 100 μm, E = 20 μm, F = 10 μm, G = 50 μm, and H = 50 μm.

Figure 2:

Morphological characteristics of cyst, vulval cone, and second-stage juvenile of Heterodera sp. (SG153). A-C: Cysts, (A) Lemon-shaped cyst (B) Anterior view (C) Underbridge level view, D-H: Second-stage juveniles (J2), (D) Entire J2 body (E) Stylet (F) Lateral field (G) Pharyngeal region (H) Tail region (black arrow: anus). *Scale bars: A = 100 μm, B = 50 μm, C = 50 μm, D = 100 μm, E = 20 μm, F = 10 μm, G = 50 μm, and H = 50 μm.
Morphological characteristics of cyst, vulval cone, and second-stage juvenile of Heterodera sp. (SG153). A-C: Cysts, (A) Lemon-shaped cyst (B) Anterior view (C) Underbridge level view, D-H: Second-stage juveniles (J2), (D) Entire J2 body (E) Stylet (F) Lateral field (G) Pharyngeal region (H) Tail region (black arrow: anus). *Scale bars: A = 100 μm, B = 50 μm, C = 50 μm, D = 100 μm, E = 20 μm, F = 10 μm, G = 50 μm, and H = 50 μm.

Figure 3:

PCR results for ITS region, 28S D2-D3, and COI gene of SG153 and SG936 nematode populations with their respective universal primers using 100 bp DNA ladder.
PCR results for ITS region, 28S D2-D3, and COI gene of SG153 and SG936 nematode populations with their respective universal primers using 100 bp DNA ladder.

Figure 4:

Phylogenetic relationship between two Heterodera populations (SG936, SG153) with selected representative sequences from many of the world known Heterodera species as inferred from Bayesian analysis of the internal transcribed spacer (ITS) sequence dataset. The newly obtained sequences were in bold. Posterior probability values more than 50% are given for appropriate clade.
Phylogenetic relationship between two Heterodera populations (SG936, SG153) with selected representative sequences from many of the world known Heterodera species as inferred from Bayesian analysis of the internal transcribed spacer (ITS) sequence dataset. The newly obtained sequences were in bold. Posterior probability values more than 50% are given for appropriate clade.

Figure 5:

Phylogenetic relationship between two Heterodera populations (SG936, SG153) with selected representative sequences from many of the world known Heterodera species as inferred from Bayesian analysis of the cytochrome c oxidase subunit I of mitochondrial DNA sequence dataset. The newly obtained sequences were in bold. Posterior probability values more than 50% are given for appropriate clade.
Phylogenetic relationship between two Heterodera populations (SG936, SG153) with selected representative sequences from many of the world known Heterodera species as inferred from Bayesian analysis of the cytochrome c oxidase subunit I of mitochondrial DNA sequence dataset. The newly obtained sequences were in bold. Posterior probability values more than 50% are given for appropriate clade.

Nematode samples used in this study.

Population code Collection date Host plant Coordinates
SG936 July 27, 2016 Rice 36.902188, 126.661624
SG153 August 16, 2017 Rice 36.901005, 126.671008

Morphometrics of second-stage juveniles and cysts for the Heterodera sp. of two populations SG153 and SG 936 as compared to H. oryzae and H. elachista in previous studies.

Population SG936, South Korea, This study SG153, South Korea, This study H. oryzae, Côte d’Ivoire, Nobbs et al. (1992) H. elachista, Japan, Nobbs et al. (1992) H. elachista, Iran, Tanha Maafi et al. (2004)
Cysts (n) 13 25 50 50 39
Length (excl. neck) 498.9 ± 96.4 (307.0-617.2) 523.3 ± 54.5 (438.9-643.3) 714.8 ± 71.5 (442-999) 446 ± 30.3 (328-557) 431 ± 48 (340-586)
Width 429.3 ± 97.3 (251.2-550.1) 360.2 ± 47.2 (288.3-447.8) 426.6 ± 53.8 (229-655) 322.6 ± 26.0 (229-449) 311 ± 48 (225-540)
Length/width 1.2 ± 0.1 (1.0-1.4) 1.5 ± 0.2 (1.3-1.8) 1.71 ± 0.12 (1.1-2.3) 1.4 ± 0.1 (1.1-1.9) 1.4 ± 0.2 (1.1-1.9)
Vulval areas (n) 11 16 10 10 10
Fenestral length 36.5 ± 4.7 (28.2-40.9) 34.2 ± 4.9 (24.8-43.6) 38.8 ± 1.85 (32-46) 29.3 ± 2.0 (23.36) 40 ± 6.5 (30-50)
Semifenestral width 34.8 ± 4.4 (29.3-44.2) 35.7 ± 4.4 (25.9-41.0) 51.6 ± 5.20 (36-69) 32.0 ± 2.5 (26-39) 31.2 ± 4.7 (26-42)
Vulval slit length 33.1 ± 5.0 (27.6-45.6) 34.3 ± 3.8 (27.8-41.3) 50.7 ± 4.0 (41-65) 37.5 ± 2.8 (26-46) 40 ± 3.8 (32-45)
Vulval bridge width 9.0 ± 4.5 (4.4-17.9) 7.2 ± 3.3 (4.0-15.0) 12.14 ± 1.9 (6-16) 5.8 ± 2.0 (2-16) –
Underbridge length 80.1 ± 7.2 (70.8-96.4) 83.0 ± 7.9 (63.4-95.3) 112.5 ± 9.0 (87-153) 72.0 ± 2.9 (65-78) 85
Vulva to anus distance 34.9 ± 3.0 (30.5-41.6) 36.6 ± 5.9 (30.1-50.3) – – –
J2 (n) 11 11 20 20 10
L 450.6 ± 16.6 (414.7-478.6) 472.4 ± 19.3 (433.1-495.1) 554 ± 9.4 (523-589) 402 ± 10.0 (377-450) 391 ± 11 (372-410)
a 23.4 ± 0.7 (22.4-24.6) 23.3 ± 1.6 (20.1-25.9) 34.0 ± 1.03 (30.1-39.3) 24.6 ± 0.9 (21.3-28.1) 22.5 ± 1.0 (20.8-24)
b – –
c 6.8 ± 0.3 (6.3-7.4) 6.8 ± 0.3 (6.3-7.2) 9.4 ± 0.3 (8.6-10.7) 7.0 ± 0.4 (5.4-8.4) 6.7 ± 0.3 (5.9-7.2)
Stylet (S) 21.1 ± 0.5 (20.4-22.4) 21.1 ± 0.8 (19.5-22.6) 20.5 ± 1.15 (16-25) 19.2 ± 0.8 (16-21) 20 ± 0.7 (18-21)
Labial region height 4.7 ± 0.5 (3.8-5.4) 4.7 ± 0.3 (4.3-5.3) – – 3
Labial region diam. 9.0 ± 0.7 (7.8-10.1) 9.1 ± 0.3 (8.7-9.6) – – 7.8 ± 0.4 (7-8)
DGO 7.3 ± 0.7 (6.5-8.4) 6.0 ± 0.8 (4.9-7.7) – – 5.2 ± 0.8 (4-7)
Anterior end to
 Excretory pore 97.5 ± 4.8 (87.5-103.0) 99.3 ± 6.6 (84.8-110.8) – – 84 ± 2.8 (79-88)
 Median bulb valve (MB) 65.4 ± 2.7 (62.1-72.6) 64.4 ± 2.2 (60.6-68.0) – – 59 ± 3.6 (52-67)
Body diam. at
 Mid-body 19.2 ± 0.8 (18.1-20.7) 20.4 ± 1.9 (18.2-24.3) 16.3 ± 0.43 (15-18) – 17.4 ± 0.8 (16-19)
 Anus (BDA) 12.6 ± 0.9 (11.7-14.5) 13.3 ± 0.8 (12.0-14.6) 12.3 ± 0.72 (10-15) 11.4 ± 0.8 (10-13) 10.2 ± 0.6 (9-11)
 Hyaline region (H) 36.9 ± 3.2 (29.3-41.0) 35.8 ± 3.4 (31.6-42.4) 32.8 ± 1.3 (26-44) – 32 ± 3.0 (25-39)
 Tail length (Tl) 66.9 ± 3.8 (60.5-73.4) 69.6 ± 4.7 (60.6-78.9) 58.8 ± 2.25 (49-66) 57.8 ± 2.9 (47-70) –
 H/S 1.7 ± 0.1 (1.4-1.9) 1.7 ± 0.2 (1.4-2.0) 1.6 ± 0.11 (1.4-2.2) – 1.6 ± 0.1 (1.3-1.9)
 L/MB 6.9 ± 0.2 (6.6-7.2) 7.3 ± 0.3 (6.9-8.1) – – 6.7 ± 0.4 (5.6-7.4)
 Tl/H 1.8 ± 0.2 (1.6-2.5) 2.0 ± 0.1 (1.7-2.2) – – –
eISSN:
2640-396X
Langue:
Anglais
Périodicité:
Volume Open
Sujets de la revue:
Life Sciences, other
RSS Feed de la revue