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Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

Jianfeng Gu
Jianfeng Gu
, 
Yiwu Fang
Yiwu Fang
 et 
Lele Liu
Lele Liu
   | 06 nov. 2020
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Publié en ligne: 06 nov. 2020
Pages: 1 - 11
DOI: https://doi.org/10.21307/jofnem-2020-105
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© 2020 Jianfeng Gu et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Light micrographs of females and males of Meloidogyne mali. A, C, E: perineal pattern; B: whole female; D: male head; E: male tail (scale bars = 10 μm).
Light micrographs of females and males of Meloidogyne mali. A, C, E: perineal pattern; B: whole female; D: male head; E: male tail (scale bars = 10 μm).

Figure 2:

Light micrographs of juveniles of Meloidogyne mali. A: whole body of a long juvenile; B: whole body of a normal juvenile; C: anterior region; D: lateral lines; E: median bulb region; F-L: tail region. (scale bars = 10 μm).
Light micrographs of juveniles of Meloidogyne mali. A: whole body of a long juvenile; B: whole body of a normal juvenile; C: anterior region; D: lateral lines; E: median bulb region; F-L: tail region. (scale bars = 10 μm).

Figure 3:

Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the D2-D3 region of the 28S rRNA gene sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold and the sequence codes are given in specimen-clone.
Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the D2-D3 region of the 28S rRNA gene sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold and the sequence codes are given in specimen-clone.

Figure 5:

The TCS haplotype networks of Meloidogyne mali (A) and the Bayesian majority rule consensus tree of Meloidogyne mali based on mitochondrial COI gene (B). A: Pie chart indicates the composition and proportion of haplotypes in each location. Each circle corresponds to one haplotype and its size is proportional to its frequency. Each line connecting the haplotypes refers to a mutational step. Marks on the lines indicate the number of steps. B: Values at branche nodes are Bayesian posterior probabilities. Newly submitted sequences are indicated in bold.
The TCS haplotype networks of Meloidogyne mali (A) and the Bayesian majority rule consensus tree of Meloidogyne mali based on mitochondrial COI gene (B). A: Pie chart indicates the composition and proportion of haplotypes in each location. Each circle corresponds to one haplotype and its size is proportional to its frequency. Each line connecting the haplotypes refers to a mutational step. Marks on the lines indicate the number of steps. B: Values at branche nodes are Bayesian posterior probabilities. Newly submitted sequences are indicated in bold.

Figure 4:

Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the ITS sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold.
Phylogenetic relationships within the genus Meloidogyne as inferred from Bayesian analysis of the ITS sequences using the GTR + I + G model. Posterior probabilities are given in clade nodes. Newly obtained sequences are indicated in bold.

Morphometrics of the described M. mali population from Japan compares to a population detected in 2013.

M. mali population with high intragenomic rRNA polymorphism M. mali population (Gu et al., 2013)
n 20 20
Body length 445  ±  28.3 (401-507) 425  ±  30.1 (362-466)
Body width at mid-body 14.0  ±  0.6 (13.1-15.4) 14.0 ± 1.1 (13.1-18.1)
Anterior end to median bulb valve 52.7 ± 4.1 (46.8-58.7) 53.5 ± 2.1 (49.5-56.8)
Anterior end to the hemizonid 79.0 ± 3.8 (74.4-85.5) –
Anterior end to the excretory pore 75.1 ± 4.2 (72.8-83.3) 74.1 ± 4.2 (68.8-82.3)
Head width 5.8 ± 0.3 (5.3-6.4) 5.1 ± 0.41 (4.1-5.7)
Head height 3.0 ± 0.2 (2.6-3.4) 2.6 ± 0.32 (2.1-3.2)
Stylet length 10.4 ± 0.5 (9.4-11.1) 10.5 ± 0.5 (9.5-11.6)
Stylet cone length 5.4 ± 0.2 (5.1-5.8) 5.7 ± 0.5 (4.7-6.7)
Stylet knob height 1.5 ± 0.4 (1.0-2.6) 1.2 ± 0.2 (0.8-1.6)
Stylet knob width 2.2 ± 0.3 (1.8-2.8) 2.1 ± 0.2 (1.8-2.7)
DGO 3.2 ± 0.5 (2.4-4.0) 4.4 ± 0.57 (3.5-5.5)
Tail length 30.5 ± 4.5 (23.5-35.8) 32.7 ± 3.0 (29.2-39.3)
Body width at the anus 7.8 ± 0.7 (6.1-8.7) 7.9 ± 0.9 (5.9-9.6)
Hyaline tail terminus length 9.8 ± 1.6 (6.8-12.0) 7.2 ± 2.3 (3.9-9.3)
a 31.9 ± 2.5 (27.4-36.6) 30.4 ± 2.6 (25.2-34.5)
b′ – 8.0 ± 0.6 (6.8-9.2)
c 14.8 ± 2.5 (12.1-19.4) 13.2 ± 1.1 (11.6-15.3)
c′ 3.8 ± 0.6 (3.0-4.8) 4.2 ± 0.7 (3.1-5.6)
m 52.4 ± 2.4 (49.5-58.6) 54.5 ± 3.8 (47-60.3)
O 30.6 ± 4.7 (22.9-38.8) 41.9 ± 5.8 (31.6-53.9)
Head width/head height 1.9 ± 0.1 (1.7-2.2) 2.0 ± 0.2 (1.6-2.3)
H% 33.2 ± 8.6 (19.1-47.3) 21.7 ± 5.9 (12.7-27.8)
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