<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>
<italic>Pratylenchus penetrans</italic> induce necrotic lesions, the hallmark symptom for the genus, soon after infection. The objective of our study was to characterize and quantify gender differences in lesion development. Independent experiments were conducted <italic>in vitro</italic> for three hosts; pea (<italic>Pisum sativum</italic> L. cv. Early Alaskan), dill (<italic>Anethum graveolens</italic> cv. Long Island Mammoth), and alfalfa (<italic>Medicago sativa</italic> cv. Vernal). Each experimental unit was an excised radical placed on water agar in a Petri dish and inoculated with either 40 adult males or 40 fourth-stage juvenile females. Length, size, and number of lesions were recorded during the experiment and the radicals were harvested 14 days after introducing nematodes. Lesions were first observed on pea after two days for female-inoculated roots, and 24 hr after introducing both genders to dill and alfalfa. Lesions expanded either by multiple lesions coalescing or individual lesions expanding over time. Males made fewer, smaller lesions with less discoloration for all three hosts. There was no difference among genders for the total number of nematodes recovered per Petri dish or the number of endoparasitic nematodes after 14 days. The survival rate of males and females at harvest was not different, indicating that the difference in lesion formation was not related to nematode population densities. This study verified and quantified the observation that lesions induced by males are less extensive and in smaller numbers than lesions by females.</p>
</abstract>

Pratylenchus penetrans induce necrotic lesions, the hallmark symptom for the genus, soon after infection. The objective of our study was to characterize and quantify gender differences in lesion development. Independent experiments were conducted in vitro for three hosts; pea (Pisum sativum L. cv. Early Alaskan), dill (Anethum graveolens cv. Long Island Mammoth), and alfalfa (Medicago sativa cv. Vernal). Each experimental unit was an excised radical placed on water agar in a Petri dish and inoculated with either 40 adult males or 40 fourth-stage juvenile females. Length, size, and number of lesions were recorded during the experiment and the radicals were harvested 14 days after introducing nematodes. Lesions were first observed on pea after two days for female-inoculated roots, and 24 hr after introducing both genders to dill and alfalfa. Lesions expanded either by multiple lesions coalescing or individual lesions expanding over time. Males made fewer, smaller lesions with less discoloration for all three hosts. There was no difference among genders for the total number of nematodes recovered per Petri dish or the number of endoparasitic nematodes after 14 days. The survival rate of males and females at harvest was not different, indicating that the difference in lesion formation was not related to nematode population densities. This study verified and quantified the observation that lesions induced by males are less extensive and in smaller numbers than lesions by females.

<article-title xml:lang="en">Difference in lesion formation by male and female <italic>Pratylenchus penetrans</italic>


Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Difference in lesion formation by male and female 


running-head

Difference in lesion formation by male and female

Pratylenchus penetrans induce necrotic lesions, the hallmark symptom for the genus, soon after infection. The objective of our study was to characterize and...

	Pea		Dill		Alfalfa
	Female	Male	Female	Male	Female	Male
Total nematodes/Petri dish	25 (14-32)	25 (15-35)	35 (29-39)	32 (28-36)	30 (26-35)	31 (26-34)
Average nematode/lesion	1.1 (0.6-1.9)	2.5** (1.6-4.2)	2.7 (2.0-4.7)	2.6 (1.0-4.3)	1.5 (1.0-2.2)	3.3** (1.7-4.6)
Endoparasites	24 (14-32)	22 (15-13)	31 (24-36)	27 (22-33)	27 (24-32)	28 (23-33)
Ectoparasites	–	–	0.6 (0-1)	1.8* (0-6)	0.5 (0-2)	1.1 (0-2)
Egressed nematodes	0.7 (0-4)	2.7* (0-12)	3.8 (2-6)	5.3 (1-12)	3.9 (1-10)	3.1 (1-6)
Survival rate (%)	–	–	97	96	100	94

Difference in lesion formation by male and female Pratylenchus penetrans

Publié en ligne: 25 août 2020

Pages: 1 - 9

DOI: https://doi.org/10.21307/jofnem-2020-090

Mots clés
Alfalfa, Dill, Lesion nematode, Pea, Symptoms

© 2020 Kanan Saikai et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Mean and range of nematode data on roots of pea, dill, and alfalfa inoculated with 40 females or 40 males.

Difference in lesion formation by male and female Pratylenchus penetrans

Publié en ligne: 25 août 2020

Pages: 1 - 9

DOI: https://doi.org/10.21307/jofnem-2020-090

Mots clésAlfalfa, Dill, Lesion nematode, Pea, Symptoms

© 2020 Kanan Saikai et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Mean and range of nematode data on roots of pea, dill, and alfalfa inoculated with 40 females or 40 males.

Mots clés
Alfalfa, Dill, Lesion nematode, Pea, Symptoms