<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In Colombia, identification of entomopathogenic nematodes (EPN’s) native species is of great importance for pest management programs. The aim of this study was to isolate and identify EPNs and their bacterial symbiont in the department of Cauca-Colombia and then evaluate the susceptibility of two Hass avocado (<italic>Persea americana</italic>) pests to the EPNs isolated. EPNs were isolated from soil samples by the insect baiting technique. Their bacterial symbiont was isolated from hemolymph of infected <italic>Galleria mellonella</italic> larvae. Both organisms were molecularly identified. Morphological, and biochemical characterization was done for the bacteria. Susceptibility of <italic>Epitrix cucumeris</italic> and <italic>Pandeleteius cinereus</italic> adults was evaluated by individually exposing adults to 50 infective juveniles. EPNs were allegedly detected at two sampled sites (natural forest and coffee cultivation) in 5.8% of the samples analyzed. However, only natural forest EPN’s could be isolated and multiplied. The isolate was identified as <italic>Steinernema carpocapsae</italic> BPS and its bacterial symbiont as <italic>Xenorhabus nematophila</italic> BPS. Adults of both pests were susceptible to <italic>S. carpocapsae</italic> indicating this EPN potential for its management. The results of this study constitute the first record of <italic>S. carpocapsae</italic> in Colombia and the susceptibility of <italic>P. cinereus</italic> to this EPN.</p>
</abstract>

In Colombia, identification of entomopathogenic nematodes (EPN’s) native species is of great importance for pest management programs. The aim of this study was to isolate and identify EPNs and their bacterial symbiont in the department of Cauca-Colombia and then evaluate the susceptibility of two Hass avocado (Persea americana) pests to the EPNs isolated. EPNs were isolated from soil samples by the insect baiting technique. Their bacterial symbiont was isolated from hemolymph of infected Galleria mellonella larvae. Both organisms were molecularly identified. Morphological, and biochemical characterization was done for the bacteria. Susceptibility of Epitrix cucumeris and Pandeleteius cinereus adults was evaluated by individually exposing adults to 50 infective juveniles. EPNs were allegedly detected at two sampled sites (natural forest and coffee cultivation) in 5.8% of the samples analyzed. However, only natural forest EPN’s could be isolated and multiplied. The isolate was identified as Steinernema carpocapsae BPS and its bacterial symbiont as Xenorhabus nematophila BPS. Adults of both pests were susceptible to S. carpocapsae indicating this EPN potential for its management. The results of this study constitute the first record of S. carpocapsae in Colombia and the susceptibility of P. cinereus to this EPN.

Isolation, identification, and pathogenicity of <italic>Steinernema carpocapsae</italic> and its bacterial symbiont in Cauca-Colombia

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Isolation, identification, and pathogenicity of  and its bacterial symbiont in Cauca-Colombia

running-head

In Colombia, identification of entomopathogenic nematodes (EPN’s) native species is of great importance for pest management programs. The aim of this study was...

Isolation, identification, and pathogenicity of Steinernema carpocapsae and its bacterial symbiont in Cauca-Colombia

Publié en ligne: 31 août 2020

Pages: 1 - 16

DOI: https://doi.org/10.21307/jofnem-2020-089

Mots clés
Biological control, Colombia,

© 2020 Esteban Neira-Monsalve et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Isolation, identification, and pathogenicity of Steinernema carpocapsae and its bacterial symbiont in Cauca-Colombia

Publié en ligne: 31 août 2020

Pages: 1 - 16

DOI: https://doi.org/10.21307/jofnem-2020-089

Mots clésBiological control, Colombia,

© 2020 Esteban Neira-Monsalve et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
Biological control, Colombia,