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Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species

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25 mai 2020
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Figure 1:

Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm).
Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm).

Figure 2:

Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Figure 3:

Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.
Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold.

Morphometrics of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province, Iran, and comparison with data of the type population and the population from France_

This study Type population, studied by Baujard (1984) French population (Baujard, 1984)
Character Females Males Female Males Females Males
n 14 9 1 10 40 5
L 829.4 ± 70.2 611.8 ± 59.6 720 840 740 750
(756–947) (502–701) (730–980) (630–950) (680–820)
a 37.4 ± 3.1 38.8 ± 3.2 33 32.5 33 33
(30.0–37.7) (33.8–39.6) (28–38) (31–36) (31–36)
b 7.2 ± 0.8 6.7 ± 0.7 9 8 8 8
(6.03–8.2) 4.3 ± 0.8 (7–9) (7–11) (7–9)
4.7 ± 0.5 (3.3–5.8)
(4–5.6) 13.9 ± 1.1
c (12.5–15.8) 14 14
2.9 ± 0.2 (13–17) (12.5–118.0)
2.9 ± 0.2 2.9 3.1
(2.7–3.1) (2.6–3.0) (2.2–3.6)
T or V 69.4 ± 2.0 90.6 ± 1.9 74 70
(70.2–72.3) (87.0–88.6) (67–74)
Cephalic region height 3.6 ± 0.4 3.0 ± 0.4
(3.0-4.3) (2.4-3.7)
Cephalic region width 8.2 ± 0.5 7.4 ± 0.6
(7.4–8.8) (6.4–8.5)
Stylet 20.9 ± 1.1 18.7 ± 1.0 23 24 23 23
(20–23) (17.3–19.8) (21–26) (20–26) (22–24)
Max. body dim. 22.4 ± 2.2 15.8 ± 1.2
(20–26) (14–18)
m 40.4 ± 5.0 44.4 ± 4.9
(32.5–44.0) (36.6–47.4)
Body width at MB 17.1 ± 1.4 15.0 ± 1.2
(16–20) (13–17)
E pore from anterior 97.5 ± 5.4 99.2 ± 8.3 104 119 105
end (92–106) (91.2–108.1) (101–132) (94–123)
Nerve ring from 100.5 ± 7.5 85.8 ± 14.2
anterior end (87–97) (71–110)
Median bulb width 11.1 ± 1.4 9.9 ± 1.5
(10–14) (8–12)
Median bulb length 20.7 ± 2.7 18.3 ± 2.3
(17–25) (16–22)
Median bulb length/ 1.9 ± 0.2 1.8± 0.1
diam. ratio (1.5–2.2) (1.7–2.0)
Ovary/testis length 360.5 ± 34.7 197.8± 6.6
(310–420) (184–201)
Hemizonid from 98 ± 3 104.4 ± 7.9 109
anterior end (92–105) (93–109) (97–115)
Vulval body diam. 22.3 ± 3.1
(17–28)
PUS length 27.6 ± 3.3 ?
(23–33)
Anal/cloacal body 15.1 ± 1.5
diam. (13–18)
Tail 44.0 ± 3.1 53
(40–49) (38–61)
Spicules arc line 20.6 ± 1.0 25.5 24
length (19–22) (22–28) (21–26)
Capitulum width 5.6 ± 0.5
(5-6)
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Sujets de la revue:
Sciences de la vie, Sciences de la vie, autres