Morphological and molecular characterization of Ektaphelenchoides pini (Massey, 1966) Baujard, 1984 (Aphelenchoididae; Ektaphelenchinae) from Iran, with morphological and taxonomic observations on some species Publié en ligne: 25 mai 2020 Pages: 1 - 12 Reçu: 02 mars 2020 © 2020 Fariba Heydari et al., published by Sciendo. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1: Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984). A: Anterior body region of male; B: Anterior body region of female; C: Metacorpus and the position of E pore (arrow) (male); D: Vulval region and PUS; E: Vulval slit in ventral view; F: Ventro-lateral view of male caudal region showing P2 (upper arrows) and P3 (lower arrows) papillae; G: Tip of ovary; H: Female posterior body region; I: Male cloacal region and spicule and tail tip (I1); J: Lateral lines; K: Spicule (close up), L: Male posterior body region showing P2 (upper arrow) and P3 (lower arrow) and single ventral P1 lacking. (Scale bars = 10 µm). Figure 2: Bayesian 50% majority rule consensus tree inferred from analysis of the partial SSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold. Figure 3: Bayesian 50% majority rule consensus tree inferred from analysis of the partial LSU rDNA sequence of Iranian population of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province under the GTR + G + I model. Bayesian posterior probability values more than 0.50 are given for appropriate clades. The new sequence is indicated in bold. Morphometrics of Ektaphelenchoides pini (Massey, 1966; Baujard, 1984) from Golestan province, Iran, and comparison with data of the type population and the population from France.
This study
Type population, studied by Baujard (1984)
French population (Baujard, 1984 )
Character
Females
Males
Female
Males
Females
Males
n
14
9
1
10
40
5
L
829.4 ± 70.2
611.8 ± 59.6
720
840
740
750
(756–947)
(502–701)
(730–980)
(630–950)
(680–820)
a
37.4 ± 3.1
38.8 ± 3.2
33
32.5
33
33
(30.0–37.7)
(33.8–39.6)
(28–38)
(31–36)
(31–36)
b
7.2 ± 0.8
6.7 ± 0.7
9
8
8
8
(6.03–8.2)
4.3 ± 0.8
(7–9)
(7–11)
(7–9)
b´
4.7 ± 0.5
(3.3–5.8)
–
–
–
–
(4–5.6)
13.9 ± 1.1
c
–
(12.5–15.8)
–
14
–
14
2.9 ± 0.2
(13–17)
(12.5–118.0)
c´
–
2.9 ± 0.2
–
2.9
–
3.1
(2.7–3.1)
(2.6–3.0)
(2.2–3.6)
T or V
69.4 ± 2.0
90.6 ± 1.9
74
–
70
–
(70.2–72.3)
(87.0–88.6)
(67–74)
Cephalic region height
3.6 ± 0.4
3.0 ± 0.4
–
–
–
–
(3.0-4.3)
(2.4-3.7)
Cephalic region width
8.2 ± 0.5
7.4 ± 0.6
–
–
–
–
(7.4–8.8)
(6.4–8.5)
Stylet
20.9 ± 1.1
18.7 ± 1.0
23
24
23
23
(20–23)
(17.3–19.8)
(21–26)
(20–26)
(22–24)
Max. body dim.
22.4 ± 2.2
15.8 ± 1.2
–
–
–
–
(20–26)
(14–18)
m
40.4 ± 5.0
44.4 ± 4.9
–
–
–
–
(32.5–44.0)
(36.6–47.4)
Body width at MB
17.1 ± 1.4
15.0 ± 1.2
–
–
–
–
(16–20)
(13–17)
E pore from anterior
97.5 ± 5.4
99.2 ± 8.3
104
119
105
end
(92–106)
(91.2–108.1)
(101–132)
(94–123)
Nerve ring from
100.5 ± 7.5
85.8 ± 14.2
–
–
–
–
anterior end
(87–97)
(71–110)
Median bulb width
11.1 ± 1.4
9.9 ± 1.5
–
–
–
–
(10–14)
(8–12)
Median bulb length
20.7 ± 2.7
18.3 ± 2.3
–
–
–
–
(17–25)
(16–22)
Median bulb length/
1.9 ± 0.2
1.8± 0.1
–
–
–
–
diam. ratio
(1.5–2.2)
(1.7–2.0)
Ovary/testis length
360.5 ± 34.7
197.8± 6.6
–
–
–
–
(310–420)
(184–201)
Hemizonid from
98 ± 3
104.4 ± 7.9
–
–
109
–
anterior end
(92–105)
(93–109)
(97–115)
Vulval body diam.
22.3 ± 3.1
–
–
–
–
–
(17–28)
PUS length
27.6 ± 3.3
–
–
–
?
–
(23–33)
Anal/cloacal body
–
15.1 ± 1.5
–
–
–
–
diam.
(13–18)
Tail
–
44.0 ± 3.1
–
–
–
53
(40–49)
(38–61)
Spicules arc line
–
20.6 ± 1.0
25.5
24
length
(19–22)
(22–28)
(21–26)
Capitulum width
–
5.6 ± 0.5
–
–
–
–
(5-6)