Molecular approach to confirm traditional identification of Radopholus similis sampled in Tanzania , , , , et | 17 mars 2020
Publié en ligne: 17 mars 2020 Pages: 1 - 8 Reçu: 02 août 2019 © 2020 Doreen M. Mgonja et al., published by Sciendo. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Figure 1: Micrographs of Radophilis similis collected from Tanzania. Representative anatomy shown for males (A-F) and females (G-L): (A, G) Entire body structure; (B, H) pharyngeal region; (C, I) posterior region; (D, J) anterior region (stylet, stylet knobs, and median bulb shown by red, yellow, and purple arrows, respectively); (E, K) posterior region (vulva and spicule shown by white arrows); and (F, L) tail region. Scale bars: 50 µm (A-G) and 25 µm (B-F, H-L). Figure 2: Bayesian consensus trees inferred for Radophilis similis. (A) Tree inferred from SSU & ITS1 rRNA. (B) Tree inferred from ITS1 and ITS2 rRNA. (C) Tree inferred from LSU rRNA. Posterior probability values >50% are shown. Sequences original to this study are labeled green, red, and blue for trees in (A), (B), and (C) respectively; sequences labeled pink represent outgroup species. Morphometrics (µm) of adult R. similis female and male populations collected from the agro-ecological zones sampled. Data are means ± SD and standard range (Roy et al., 2018; Elbadri et al., 1999) in parentheses.
Female populations
Male populations
Parametera
Lake zone
Northern zone
Southern Highlands zone
Zanzibar islands
Lake zone
Northern zone
Southern Highlands zone
Zanzibar islands
L
520.5 ± 11.4
564.1 ± 11.4
554.3 ± 11.4
581.0 ± 11.4
486.3 ± 12.2
566.1 ± 12.2
542.8 ± 12.2
570.5 ± 12.2
(500 – 600)
(500–600)
(500 – 600)
(500 – 600)
(440 – 685)
(440–685)
(440 – 685)
(440 – 685)
a
28.5 ± 5.1
28.5 ± 5.1
27.3 ± 3.9
30.3 ± 2.7
29.5 ± 3.8
31.8 ± 5.2
33.1 ± 3.6
33.1 ± 3.6
(20 – 34.0)
(20 – 34.0)
(20 – 34.0)
(20 – 34.0)
(24.5 – 42.5)
(24.5 – 42.5)
(24.5 – 42.5)
(24.5 – 42.5)
b
6.3 ± 1.1
4.93 ± 0.7
5.79 ± 0.7
5.6 ± 0.8
5.2 ± 0.3
5.3 ± 0.5
5.4 ± 0.3
5.1 ± 0.2
(4.2 – 6.6)
(4.2 – 6.6)
(4.2 – 6.6)
(4.2 – 6.6)
(4.1 – 5.6)
(4.1 – 5.6)
(4.1 – 5.6)
(4.1 – 5.6)
b'
5.1 ± 0.5
4.2 ± 0.6
4.9 ± 0.5
4.8 ± 0.6
5.4 ± 0.5
5.4 ± 0.7
5.5 ± 0.8
5.9 ± 0.7
(3.3 – 5.7)
(3.3 – 5.7)
(3.3 – 5.7)
(3.3 – 5.7)
(3.7 – 6.8)
(3.7 – 6.8)
(3.7 – 6.8)
(3.7 – 6.8)
c
8.2 ± 1.0
7.9 ± 0.5
7.5 ± 0.5
8.4 ± 1.2
6.8 ± 0.3
6.8 ± 0.6
7.3 ± 0.1
6.9 ± 0.6
(6.8 – 12.2)
(6.8 – 12.2)
(6.8 – 12.2)
(6.8 – 12.2)
(6.3 – 10.3)
(6.3 – 10.3)
(6.3 – 10.3)
(6.3 – 10.3)
c'
4.4 ± 0.7
4.2 ± 1.0
5.12 ± 0.8
4.8 ± 0.5
5.8 ± 0.7
5.7 ± 1.0
5.8 ± 1.1
6.4 ± 0.7
(2.8 – 6.2)
(2.8 – 6.2)
(2.8 – 6.2)
(2.8 – 6.2)
(3.7 – 6.9)
(3.7 – 6.9)
(3.7 – 6.9)
(3.7 – 6.9)
Sl
10.8 ± 1.1
10.6 ± 1.1
10.7 ± 1.1
10.4 ± 1.1
10.3 ± 2.2
10.0 ± 2.0
9.8 ± 2.2
8.7 ± 2.2
(14 – 18)
(14 – 18)
(14 – 18)
(14 – 18)
(8 – 13)
(8 – 13)
(8 – 13)
(8 – 13)
V
51 ± 0.9
55.5 ± 0.9
55.4 ± 0.8
58.2 ± 0.7
131.7 ± 2.1
144.0 ± 2.1
131.6 ± 2.2
131.7 ± 2.1
(50.7 – 59)
(50.7 – 59)
(50.7 – 59)
(50.7 – 59)
(128 – 250)
(128 – 250)
(128 – 250)
(128 – 250)
W
16.0 ± 3.0
18.0 ± 3.0
17.6 ± 3.0
18.8 ± 3.0
14.5 ± 2.0
16.1 ± 2.1
14.7 ± 2.1
14. 7 ± 2.3
(13 – 21)
(13 – 21)
(13 – 21)
(13 – 21)
(14 – 18)
(14 – 18)
(14 – 18)
(14 – 18)
t
64.7 ± 2.0
73.3 ± 2.3
75.2 ± 2.0
72.3 ± 2.6
69.2 ± 2.0
81.8 ± 1.9
72.0 ± 2.1
80.5 ± 2.0
(52 – 100)
(52 – 100)
(52 – 100)
(52 – 100)
(60 – 90)
(60 – 90)
(60 – 90)
(60 – 90)
OL
72.5 ± 2.6
91.8 ± 2.6
82.7 ± 3.1
84.8 ± 3.6
63.8 ± 2.1
80.1 ± 2.2
76.4 ± 2.2
72.5 ± 2.0
(69 – 99)
(69 – 99)
(69 – 99)
(69 – 99)
(62 – 123)
(62 – 123)
(62 – 123)
(62 – 123)
DGO
3.0 ± 0.4
3.5 ± 0.5
3.2 ± 0.5
2.6 ± 0.5
1.8 ± 0.1
2.4 ± 0.5
2.6 ± 0.3
1.5 ± 0.3
(2.0 – 5.5)
(2.0 – 5.5)
(2.0 – 5.5)
(2.0 – 5.5)
(1.5–3.0)
(1.5 – 3.0)
(1.5 – 3.0)
(1.5 – 3.0)