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Figure 1
Complementation by expression of HgBioB in biotin synthase-deficient E. coli strain. (A) HgBioB construct diagrams showing polymorphic amino acids, from N-terminus to C-terminus, at positions 24 and 44. HgBioB-vir was mutagenized to produce HgBioB-vir-M1, by generating an alanine to proline mutation at position 24 (A24P); and HgBioB-vir-M2, by generating a glutamine to arginine mutation at position 44 (Q44R). (B) The expression of the vector control, HgBioB-avr, HgBioB-vir, and two mutagenized forms of the virulent allele HgBioB-vir-M1 and HgBioB-vir-M2 on minimal media with and without supplemental biotin.
Figure 2
Box plots of HgBioB amino acid polymorphism trends among SCN field populations, showing the percentage of amino acid differences for A24P (A) and Q44R (B).
Figure 3
Scatterplots showing the HgBioB avirulent and virulent polymorphic amino acid pairs. (A) The avirulent pair shows a positive linear relationship between amino acid positions P24 and R44. (B) Similarly, the virulent pair also shows a positive linear relationship between amino acid positions A24 and Q44.