<abstract xmlns="http://www.w3.org/1999/xhtml">ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We describe the application of the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) method, which is considered to be more versatile for large sample numbers, compared with conventional ABO genotyping by PCR-RFLP. PCR-SSO, while maintaining accurate and reliable results, reduces costs and labor. A population of 155 random individuals was investigated for the three O alleles, <italic>O1, O1*,</italic> and <italic>O2</italic>. The allelic frequencies were 35 percent, 26 percent, and 5 percent, respectively. PCR-SSO results correlated completely with both serologic and PCR-RFLP results.</abstract>

ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We describe the application of the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) method, which is considered to be more versatile for large sample numbers, compared with conventional ABO genotyping by PCR-RFLP. PCR-SSO, while maintaining accurate and reliable results, reduces costs and labor. A population of 155 random individuals was investigated for the three O alleles, O1, O1*, and O2. The allelic frequencies were 35 percent, 26 percent, and 5 percent, respectively. PCR-SSO results correlated completely with both serologic and PCR-RFLP results.

ABO genotyping—identification of <italic>O1, O1*</italic>, and <italic>O2</italic> alleles using the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) technique

Immunohematology

ABO genotyping—identification of , and  alleles using the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) technique

ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We...

ABO genotyping—identification of O¹, O^1*, and O² alleles using the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) technique

Publié en ligne: 16 nov. 2020

Pages: 149 - 153

DOI: https://doi.org/10.21307/immunohematology-2019-769

© 1996 N.A. Mifsud et al., published by Sciendo

ABO genotyping—identification of O1, O1*, and O2 alleles using the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) technique

Publié en ligne: 16 nov. 2020

Pages: 149 - 153

DOI: https://doi.org/10.21307/immunohematology-2019-769

© 1996 N.A. Mifsud et al., published by Sciendo

ABO genotyping—identification of O¹, O^1*, and O² alleles using the polymerase chain reaction–sequence specific oligonucleotide (PCR-SSO) technique