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<bold>Introduction</bold>: Experimental studies have confirmed the potential vasculoprotective effects of melatonin. The aim of the study was to evaluate the influence of melatonin and its modulative role on 7-ketocholesterol-induced changes in human aortic endothelial cells (HAECs) and human aortic smooth muscle cells (HuAoSMCs).
<bold>Materials and methods</bold>: The real-time cell electric impedance sensing (xCELLigence) system was used to measure cell impedance, while flow cytometry was employed to assess viability and apoptosis.
<bold>Results</bold>: It was found that melatonin did not change the impedance and viability of HuAoSMCs, nor did it inhibit their 7-ketocholesterol- induced apoptosis. Melatonin decreased the impedance of HAECs. However, this effect was not attributed to cell apoptosis. In addition, it did not prevent the 7-ketocholesterol-induced decrease in HAEC impedance. Nevertheless, pretreatment with melatonin at concentrations of 10 nM and 100 nM attenuated the apoptosis caused by 7-ketocholesterol.
<bold>Conclusions</bold>: This study confirmed the toxic effect of 7-ketocholesterol on primary human vascular wall cells, using 2 independent methods. We have demonstrated that melatonin has no barrier-protective effect, but low concentrations of melatonin may counteract the toxic effect of 7-ketocholesterol on HAECs through the inhibition of apoptosis. Melatonin has no significant effect on the impedance and viability of HuAoSMCs.
</abstract>

Introduction: Experimental studies have confirmed the potential vasculoprotective effects of melatonin. The aim of the study was to evaluate the influence of melatonin and its modulative role on 7-ketocholesterol-induced changes in human aortic endothelial cells (HAECs) and human aortic smooth muscle cells (HuAoSMCs).
Materials and methods: The real-time cell electric impedance sensing (xCELLigence) system was used to measure cell impedance, while flow cytometry was employed to assess viability and apoptosis.
Results: It was found that melatonin did not change the impedance and viability of HuAoSMCs, nor did it inhibit their 7-ketocholesterol- induced apoptosis. Melatonin decreased the impedance of HAECs. However, this effect was not attributed to cell apoptosis. In addition, it did not prevent the 7-ketocholesterol-induced decrease in HAEC impedance. Nevertheless, pretreatment with melatonin at concentrations of 10 nM and 100 nM attenuated the apoptosis caused by 7-ketocholesterol.
Conclusions: This study confirmed the toxic effect of 7-ketocholesterol on primary human vascular wall cells, using 2 independent methods. We have demonstrated that melatonin has no barrier-protective effect, but low concentrations of melatonin may counteract the toxic effect of 7-ketocholesterol on HAECs through the inhibition of apoptosis. Melatonin has no significant effect on the impedance and viability of HuAoSMCs.

The effect of melatonin on the barrier properties of human primary aortic endothelial and human aortic smooth muscle cells based on the real-time cell electric impedance sensing system

Department of Internal Diseases and Clinical Pharmacology, The Laboratory of Tissue Immunopharmacology

Pomeranian Journal of Life Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction: Experimental studies have confirmed the potential vasculoprotective effects of melatonin. The aim of the study was to evaluate the influence of...

The effect of melatonin on the barrier properties of human primary aortic endothelial and human aortic smooth muscle cells based on the real-time cell electric impedance sensing system

Publié en ligne: 16 juil. 2023

Pages: 9 - 15

DOI: https://doi.org/10.21164/pomjlifesci.928

Mots clés
melatonin, 7-ketocholesterol, HAEC, HuAoSMc, apoptosis, impedance

© 2023 Roksana Dorantowicz-Jezierska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The effect of melatonin on the barrier properties of human primary aortic endothelial and human aortic smooth muscle cells based on the real-time cell electric impedance sensing system

Publié en ligne: 16 juil. 2023

Pages: 9 - 15

DOI: https://doi.org/10.21164/pomjlifesci.928

Mots clésmelatonin, 7-ketocholesterol, HAEC, HuAoSMc, apoptosis, impedance

© 2023 Roksana Dorantowicz-Jezierska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
melatonin, 7-ketocholesterol, HAEC, HuAoSMc, apoptosis, impedance