<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Adventitious root development is crucial for cutting propagation in hybrid larch. In the present study, semilignified cuttings of Larix kaempferi × L. olgensis clones were used to study the morphological and anatomical changes during the different development stages and compare the changes in contents of endogenous phytohormone in two clones with evidently different rooting capacity (25-5 and 23-12), and the effects of exogenous Indole-3-Butyric Acid (IBA) on phytohormone content of a very low native rooting capacity clone (8-9) during the development of adventitious roots. The results showed that the development of adventitious root in semi-lignified cuttings involves three key stages: root cell dedifferentiation and division (14-18 DAC); meristem formation and development (25-35 DAC); root formation and elongation (50-55 DAC). The endogenous phytohormones play a key role during adventitious root development. For 25-5 and 23-12, the level of endogenous phytohormones, especially the ABA content, has an important effect on rooting capacity. For the effects of IBA on 8-9, except lower ABA content, the temporary peak of IAA on the 1 DAC was important for initiating the rooting process. The ratio of (IAA + GA3 + ZR)/ABA can be used as an indicator of rooting capacity. The anatomical and physiological data described here for semi-lignified cuttings of L. kaempferi × L. olgensis clones improves our knowledge of adventitious roots development. Our study will also benefit future research on examining the molecular mechanisms of the adventitious roots development in hybrid larch. </p></abstract>

Adventitious root development is crucial for cutting propagation in hybrid larch. In the present study, semilignified cuttings of Larix kaempferi × L. olgensis clones were used to study the morphological and anatomical changes during the different development stages and compare the changes in contents of endogenous phytohormone in two clones with evidently different rooting capacity (25-5 and 23-12), and the effects of exogenous Indole-3-Butyric Acid (IBA) on phytohormone content of a very low native rooting capacity clone (8-9) during the development of adventitious roots. The results showed that the development of adventitious root in semi-lignified cuttings involves three key stages: root cell dedifferentiation and division (14-18 DAC); meristem formation and development (25-35 DAC); root formation and elongation (50-55 DAC). The endogenous phytohormones play a key role during adventitious root development. For 25-5 and 23-12, the level of endogenous phytohormones, especially the ABA content, has an important effect on rooting capacity. For the effects of IBA on 8-9, except lower ABA content, the temporary peak of IAA on the 1 DAC was important for initiating the rooting process. The ratio of (IAA + GA3 + ZR)/ABA can be used as an indicator of rooting capacity. The anatomical and physiological data described here for semi-lignified cuttings of L. kaempferi × L. olgensis clones improves our knowledge of adventitious roots development. Our study will also benefit future research on examining the molecular mechanisms of the adventitious roots development in hybrid larch.

Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Adventitious root development is crucial for cutting propagation in hybrid larch. In the present study, semilignified cuttings of Larix kaempferi × L. olgensis...

Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

Publié en ligne: 01 juil. 2017

Pages: 96 - 103

Reçu: 07 juin 2012

DOI: https://doi.org/10.1515/sg-2013-0012

Mots clés
anatomy, IBA, phytohormone ratio, adventitious roots, rooting capacity, Larix kaempferi × L. olgensis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Anatomical and Physiological Effects of Phytohormones on Adventitious Roots Development in Larix kaempferi × L. olgensis

Publié en ligne: 01 juil. 2017

Pages: 96 - 103

Reçu: 07 juin 2012

DOI: https://doi.org/10.1515/sg-2013-0012

Mots clésanatomy, IBA, phytohormone ratio, adventitious roots, rooting capacity, Larix kaempferi × L. olgensis

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
anatomy, IBA, phytohormone ratio, adventitious roots, rooting capacity, Larix kaempferi × L. olgensis