<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been described earlier showing strong differentiation between S. leprosula and S. parvifolia. The AFLP markers were isolated from a gel, re-amplified, cloned and sequenced. Primer sets were designed from these sequences and AFLP fragments were converted into SCAR markers. The SCAR markers and PCR-RFLP markers of the chloroplast region trnLF digested with HinfI were used to screen in total 557 samples of S. parvifolia and S. leprosula from nineteen widely separated populations in Indonesia. Complete genetic differentiation between species was observed based on the putatively nuclear SCAR marker and the PCR-RFLP of the cpDNA region. We found a good agreement between leaf morphological variation and species identification based on both marker types and no indication for interspecific hybridization.</p></abstract>

The development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been described earlier showing strong differentiation between S. leprosula and S. parvifolia. The AFLP markers were isolated from a gel, re-amplified, cloned and sequenced. Primer sets were designed from these sequences and AFLP fragments were converted into SCAR markers. The SCAR markers and PCR-RFLP markers of the chloroplast region trnLF digested with HinfI were used to screen in total 557 samples of S. parvifolia and S. leprosula from nineteen widely separated populations in Indonesia. Complete genetic differentiation between species was observed based on the putatively nuclear SCAR marker and the PCR-RFLP of the cpDNA region. We found a good agreement between leaf morphological variation and species identification based on both marker types and no indication for interspecific hybridization.

Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification...

Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)

Publié en ligne: 20 oct. 2017

Pages: 249 - 257

Reçu: 19 mai 2010

DOI: https://doi.org/10.1515/sg-2010-0035

Mots clés
SCAR, Dipterocarpaceae, molecular identification, tropical timber, species assignment, hybridization

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Development of SCAR Markers for Species Identification in the Genus Shorea (Dipterocarpaceae)

Publié en ligne: 20 oct. 2017

Pages: 249 - 257

Reçu: 19 mai 2010

DOI: https://doi.org/10.1515/sg-2010-0035

Mots clésSCAR, Dipterocarpaceae, molecular identification, tropical timber, species assignment, hybridization

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
SCAR, Dipterocarpaceae, molecular identification, tropical timber, species assignment, hybridization