<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The thermal profile for polymerase chain reaction (PCR) technique is the main concern in obtaining the PCR product. Determination of optimal thermal profile can be achieved by various techniques, the main objectives of which are simplicity and cost effectiveness of the method. Real-time PCR is a novel molecular biology method widely used in different fields, recognised by its accuracy and precision. In the present work, the thermal profile was estimated for DNA from air-dried collection material of <italic>Notiophilus</italic> genus and new sequences were obtained for Gene-Bank. Air-dried entomological collections exist around the world and the use of DNA from such type of material is widespread and offers various unique preferences. The purpose of the study was to assess the suitability of different primer annealing temperatures (42 °C – 55 °C) for universal mitochondrial DNA COI gene primer pair HCO/LCO using real-time PCR method. During this study we demonstrate that real-time PCR could be useful to determine the optimal thermal profile for low-yield DNA for increasing the number of qualitative DNA sequences.</p></abstract>

The thermal profile for polymerase chain reaction (PCR) technique is the main concern in obtaining the PCR product. Determination of optimal thermal profile can be achieved by various techniques, the main objectives of which are simplicity and cost effectiveness of the method. Real-time PCR is a novel molecular biology method widely used in different fields, recognised by its accuracy and precision. In the present work, the thermal profile was estimated for DNA from air-dried collection material of Notiophilus genus and new sequences were obtained for Gene-Bank. Air-dried entomological collections exist around the world and the use of DNA from such type of material is widespread and offers various unique preferences. The purpose of the study was to assess the suitability of different primer annealing temperatures (42 °C – 55 °C) for universal mitochondrial DNA COI gene primer pair HCO/LCO using real-time PCR method. During this study we demonstrate that real-time PCR could be useful to determine the optimal thermal profile for low-yield DNA for increasing the number of qualitative DNA sequences.

Real-Time Polymerase Chain Reaction For Detection Of Optimal Thermal Profile For DNA Templates From Air-Dried Insect Collection Material

Institute of Life Sciences and Technologies, Daugavpils University, Parādes iela 1A, Daugavpils, LV-5401, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

REĀLA LAIKA POLIMERĀZES ĶĒDES REAKCIJA OPTIMĀLĀ TERMISKA PROFILA NOTEIKŠANAI DNS PARAUGIEM NO SAUSĀ KUKAIŅU KOLEKCIJU MATERIĀLA

The thermal profile for polymerase chain reaction (PCR) technique is the main concern in obtaining the PCR product. Determination of optimal thermal profile...

Real-Time Polymerase Chain Reaction For Detection Of Optimal Thermal Profile For DNA Templates From Air-Dried Insect Collection Material

Publié en ligne: 14 juil. 2015

Pages: 33 - 37

Reçu: 05 janv. 2015

DOI: https://doi.org/10.1515/prolas-2015-0003

Mots clés
rtPCR conditions, COI gene, mtDNA,

© Inese Kokina et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Real-Time Polymerase Chain Reaction For Detection Of Optimal Thermal Profile For DNA Templates From Air-Dried Insect Collection Material

Publié en ligne: 14 juil. 2015

Pages: 33 - 37

Reçu: 05 janv. 2015

DOI: https://doi.org/10.1515/prolas-2015-0003

Mots clésrtPCR conditions, COI gene, mtDNA,

© Inese Kokina et al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
rtPCR conditions, COI gene, mtDNA,