<abstract xmlns="http://www.w3.org/1999/xhtml">

Phytoplankton modify the optical properties of the seawater by altering the subsurface light field. Information on the accessory pigments present in the phytoplankton helps to differentiate major phytoplankton classes or taxonomic groups. The variability in the absorption spectra of phytoplankton and particulate matter of case 2 coastal waters of the Southeastern Arabian Sea were studied from June 2010 to November 2011. The phytoplankton specific absorption coefficient, at 440 nm and 675 nm, a*ph (440) and a*ph (675) varied from 0.018 to 0.32 m2 mg-1 and from 0.0005 to 0.16 m2 mg-1, respectively. The 4th derivative spectra computed for each in vivo absorption spectrum showed that the amplitude of maxima obtained is proportional to the concentration of the chromoprotein which absorbed that wavelength. Regression of pigment concentration against the 4th derivative spectral coefficient showed that the measurements of particulate absorption could provide quantitative information on chlorophyll α and other accessory pigment concentrations. Fucoxanthin and diadinoxanthin, the carotenoid pigments found in the diatoms were identified from the derivatives peaks. The study demonstrates the utility of using the 4th derivative analysis as a tool to identify the dominating phytoplankton group and its pigment composition.
</abstract>

Phytoplankton modify the optical properties of the seawater by altering the subsurface light field. Information on the accessory pigments present in the phytoplankton helps to differentiate major phytoplankton classes or taxonomic groups. The variability in the absorption spectra of phytoplankton and particulate matter of case 2 coastal waters of the Southeastern Arabian Sea were studied from June 2010 to November 2011. The phytoplankton specific absorption coefficient, at 440 nm and 675 nm, a*ph (440) and a*ph (675) varied from 0.018 to 0.32 m2 mg-1 and from 0.0005 to 0.16 m2 mg-1, respectively. The 4th derivative spectra computed for each in vivo absorption spectrum showed that the amplitude of maxima obtained is proportional to the concentration of the chromoprotein which absorbed that wavelength. Regression of pigment concentration against the 4th derivative spectral coefficient showed that the measurements of particulate absorption could provide quantitative information on chlorophyll α and other accessory pigment concentrations. Fucoxanthin and diadinoxanthin, the carotenoid pigments found in the diatoms were identified from the derivatives peaks. The study demonstrates the utility of using the 4th derivative analysis as a tool to identify the dominating phytoplankton group and its pigment composition.

Decomposition study of in vivo phytoplankton absorption spectra aimed at identifying the pigments and the phytoplankton group in complex case 2 coastal waters of the Arabian Sea

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phytoplankton modify the optical properties of the seawater by altering the subsurface light field. Information on the accessory pigments present in the...

Decomposition study of in vivo phytoplankton absorption spectra aimed at identifying the pigments and the phytoplankton group in complex case 2 coastal waters of the Arabian Sea

Publié en ligne: 30 sept. 2015

Pages: 282 - 293

Reçu: 20 nov. 2014

Accepté: 18 mars 2015

DOI: https://doi.org/10.1515/ohs-2015-0027

Mots clés
Total particulate absorption, phytoplankton specific absorption coefficient, packaging effect, derivative analysis

© 2015 Faculty of Oceanography and Geography, University of Gdańsk, Poland

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Decomposition study of in vivo phytoplankton absorption spectra aimed at identifying the pigments and the phytoplankton group in complex case 2 coastal waters of the Arabian Sea

Publié en ligne: 30 sept. 2015

Pages: 282 - 293

Reçu: 20 nov. 2014

Accepté: 18 mars 2015

DOI: https://doi.org/10.1515/ohs-2015-0027

Mots clésTotal particulate absorption, phytoplankton specific absorption coefficient, packaging effect, derivative analysis

© 2015 Faculty of Oceanography and Geography, University of Gdańsk, Poland

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Total particulate absorption, phytoplankton specific absorption coefficient, packaging effect, derivative analysis