<abstract xmlns="http://www.w3.org/1999/xhtml">A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A remote-controlled synthesizer Synthra RNplus was used for nucleophilic substitution of NITTP (1′-(2′-nitro-1-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonyl-propanediol) with 18F anion. Labeling of 5 mg of precursor was performed in anhydrous acetonitrile at 100°C for 10 min, and the hydrolysis with HCl was performed at 100°C for 5 min. Final purification was done with high-performance liquid chromatography (HPLC) and the radiochemical purity of radiotracer was higher than 99%. Proposed [18F]FMISO synthesis was used as a reliable tool in studies on hypoxia in Lewis lung carcinoma (LLC) in mouse models.</abstract>

A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A remote-controlled synthesizer Synthra RNplus was used for nucleophilic substitution of NITTP (1′-(2′-nitro-1-imidazolyl)-2-O-tetrahydropyranyl-3-O-toluenesulfonyl-propanediol) with 18F anion. Labeling of 5 mg of precursor was performed in anhydrous acetonitrile at 100°C for 10 min, and the hydrolysis with HCl was performed at 100°C for 5 min. Final purification was done with high-performance liquid chromatography (HPLC) and the radiochemical purity of radiotracer was higher than 99%. Proposed [18F]FMISO synthesis was used as a reliable tool in studies on hypoxia in Lewis lung carcinoma (LLC) in mouse models.

Imaging of hypoxia in small animals with 18F fluoromisonidasole

Heavy Ion Laboratory, University of Warsaw, 5A Pasteura Str., 02-093 Warsaw, Poland

Faculty of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland

Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland

Faculty of Physics, University of Warsaw, 5 Pasteura Str., 02-093 Warsaw, Poland

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Imaging of hypoxia in small animals with F fluoromisonidasole

A method of automated synthesis of [18F]fluoromisonidazole ([18F]FMISO) for application in preclinical studies on small animals was presented. A...

Imaging of hypoxia in small animals with ¹⁸F fluoromisonidasole

Publié en ligne: 15 juin 2016

Pages: 219 - 223

Reçu: 30 sept. 2015

Accepté: 09 mars 2016

DOI: https://doi.org/10.1515/nuka-2016-0037

© by Krzysztof Kilian

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.