<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage, in order to rapidly identify individuals, who require clinical treatment. Accurate dose estimates can be made by biological dosimetry, to predict the acute radiation syndrome (ARS) within days after a radiation accident or a malicious act involving radiation. Timely information on dose is important for the medical management of acutely irradiated persons [1]. The aim of the study was to evaluate the usefulness of the micronuclei (MNi) scoring procedure in an experimental mode, where 500 binucleated cells were analyzed in different exposure dose ranges. Whole-body exposure was simulated in an in vitro experiment by irradiating whole blood collected from one healthy donor with 60 MeV protons and 250 keV X-rays, in the dose range of 0.3-4.0 Gy. For achieving meaningful results, sample scoring was performed by three independent persons, who followed guidelines described in detail by Fenech et al. [2, 3]. Compared results revealed no significant differences between scorers, which has important meaning in reducing the analysis time. Moreover, presented data based on 500 cells distribution, show that there are significant differences between MNi yields after 1.0 Gy exposure of blood for both protons and X-rays, implicating this experimental mode as appropriate for the distinction between high and low dose-exposed individuals, which allows early classification of exposed victims into clinically relevant subgroups.</p></abstract>

Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage, in order to rapidly identify individuals, who require clinical treatment. Accurate dose estimates can be made by biological dosimetry, to predict the acute radiation syndrome (ARS) within days after a radiation accident or a malicious act involving radiation. Timely information on dose is important for the medical management of acutely irradiated persons [1]. The aim of the study was to evaluate the usefulness of the micronuclei (MNi) scoring procedure in an experimental mode, where 500 binucleated cells were analyzed in different exposure dose ranges. Whole-body exposure was simulated in an in vitro experiment by irradiating whole blood collected from one healthy donor with 60 MeV protons and 250 keV X-rays, in the dose range of 0.3-4.0 Gy. For achieving meaningful results, sample scoring was performed by three independent persons, who followed guidelines described in detail by Fenech et al. [2, 3]. Compared results revealed no significant differences between scorers, which has important meaning in reducing the analysis time. Moreover, presented data based on 500 cells distribution, show that there are significant differences between MNi yields after 1.0 Gy exposure of blood for both protons and X-rays, implicating this experimental mode as appropriate for the distinction between high and low dose-exposed individuals, which allows early classification of exposed victims into clinically relevant subgroups.

Application of the micronucleus assay performed by different scorers in case of large-scale radiation accidents

Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 11 Łojasiewicza Str., 30-348 Krakow, Poland, Tel.: +48 883 740 081

Department of Experimental Physics of Complex Systems, The Henryk Niewodniczański Institute of Nuclear Physics of the Polish Academy of Sciences, 152 Radzikowskiego Str., 31-342 Krakow, Poland

Cyclotron Center Bronowice, Proton Radiotherapy Group, The Henryk Niewodniczański Institute of Nuclear Physics of the Polish Academy of Sciences, 152 Radzikowskiego Str., 31-342, Krakow, Poland

Nukleonika

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mass casualty scenarios of radiation exposure require high throughput biological dosimetry techniques for population triage, in order to rapidly identify...

Application of the micronucleus assay performed by different scorers in case of large-scale radiation accidents

Publié en ligne: 25 sept. 2015

Pages: 643 - 649

Reçu: 24 sept. 2014

Accepté: 20 mai 2015

DOI: https://doi.org/10.1515/nuka-2015-0105

Mots clés
biological dosimetry · cytokinesis-blocked micronucleus assay · frequency of micronuclei

© by Kamila Rawojć

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Application of the micronucleus assay performed by different scorers in case of large-scale radiation accidents

Publié en ligne: 25 sept. 2015

Pages: 643 - 649

Reçu: 24 sept. 2014

Accepté: 20 mai 2015

DOI: https://doi.org/10.1515/nuka-2015-0105

Mots clésbiological dosimetry · cytokinesis-blocked micronucleus assay · frequency of micronuclei

© by Kamila Rawojć

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
biological dosimetry · cytokinesis-blocked micronucleus assay · frequency of micronuclei