<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin.</p></abstract>

The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin.

Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study

Department of Biology, Faculty of Medicine

Journal of Biomedical and Clinical Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent...

Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study

Publié en ligne: 30 déc. 2017

Pages: 37 - 39

Reçu: 06 juin 2017

Accepté: 02 nov. 2017

DOI: https://doi.org/10.1515/jbcr-2017-0007

Mots clés
nuclear matrix, intermediate filaments, anti-ovarian antibodies, immunological infertility, ELISA

© 2017 Stefka M. Delimitreva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study

Publié en ligne: 30 déc. 2017

Pages: 37 - 39

Reçu: 06 juin 2017

Accepté: 02 nov. 2017

DOI: https://doi.org/10.1515/jbcr-2017-0007

Mots clésnuclear matrix, intermediate filaments, anti-ovarian antibodies, immunological infertility, ELISA

© 2017 Stefka M. Delimitreva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
nuclear matrix, intermediate filaments, anti-ovarian antibodies, immunological infertility, ELISA