<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In the northern areas of Pakistan, the use of <italic>Artemisia</italic> based therapeutics is a common practice. Plants of genus <italic>Artemisia</italic> are known to possess anthelmintic and therapeutic effect. Infections caused by gastrointestinal nematodes are major threat to livestock industry across the world resulting in loss of production and indirect economic losses due to high cost of anthelmintic drugs. Present study was carried out to evaluate <italic>in vitro</italic> and <italic>in vivo</italic> effect of <italic>Artemisia sieversiana</italic> and <italic>Artemisia parviflora</italic> on <italic>Haemonchus contortus,</italic> a parasitic nematode of small ruminants. Methanolic plant extract was tested against three different developmental stages using an egg hatch assay, infective larvae and adult worm motility assay. Different concentrations were used for the bioassays and post exposure mortality was recorded after 8 hr for adult worms and infective larvae, while egg inhibition percentage was observed after 27 hr. A highly significant ability to inhibit the egg hatching (100 %) was recorded for both plant extracts while, the highest activity for adult worm assay and larvicidal assay was 90 % for <italic>A. sieversiana</italic>. The highest activity for adult motility and larvicidal assay for <italic>A. parviflora</italic> was 89 % and 86.6 % respectively. For <italic>in vivo</italic> trials maximum parentage reduction was 77.0 % for <italic>A. sieversiana</italic> and 73.6 % for <italic>A. parviflora</italic>. It is concluded that selected plant extracts were effective in reducing worm burden in animals.</p></abstract>

In the northern areas of Pakistan, the use of Artemisia based therapeutics is a common practice. Plants of genus Artemisia are known to possess anthelmintic and therapeutic effect. Infections caused by gastrointestinal nematodes are major threat to livestock industry across the world resulting in loss of production and indirect economic losses due to high cost of anthelmintic drugs. Present study was carried out to evaluate in vitro and in vivo effect of Artemisia sieversiana and Artemisia parviflora on Haemonchus contortus, a parasitic nematode of small ruminants. Methanolic plant extract was tested against three different developmental stages using an egg hatch assay, infective larvae and adult worm motility assay. Different concentrations were used for the bioassays and post exposure mortality was recorded after 8 hr for adult worms and infective larvae, while egg inhibition percentage was observed after 27 hr. A highly significant ability to inhibit the egg hatching (100 %) was recorded for both plant extracts while, the highest activity for adult worm assay and larvicidal assay was 90 % for A. sieversiana. The highest activity for adult motility and larvicidal assay for A. parviflora was 89 % and 86.6 % respectively. For in vivo trials maximum parentage reduction was 77.0 % for A. sieversiana and 73.6 % for A. parviflora. It is concluded that selected plant extracts were effective in reducing worm burden in animals.

<italic>In vitro</italic> and <italic>in vivo</italic> anthelmintic activity of extracts from <italic>Artemisia parviflora</italic> and <italic>A. sieversiana</italic>

General Karol Kaczkowski Military Institute of Hygiene and Epidemiology

Department of Parasitology, Institute of Zoology, Faculty of Biology

Department of Parasitology, Faculty of Veterinary Medicine

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 and  anthelmintic activity of extracts from  and 

In the northern areas of Pakistan, the use of Artemisia based therapeutics is a common practice. Plants of genus Artemisia are known to possess anthelmintic...

In vitro and in vivo anthelmintic activity of extracts from Artemisia parviflora and A. sieversiana

Publié en ligne: 02 août 2017

Pages: 218 - 224

Reçu: 09 janv. 2017

Accepté: 19 avr. 2017

DOI: https://doi.org/10.1515/helm-2017-0028

Mots clés
plants, Anthelmintics,

© 2017 S. Irum et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In vitro and in vivo anthelmintic activity of extracts from Artemisia parviflora and A. sieversiana

Publié en ligne: 02 août 2017

Pages: 218 - 224

Reçu: 09 janv. 2017

Accepté: 19 avr. 2017

DOI: https://doi.org/10.1515/helm-2017-0028

Mots clésplants, Anthelmintics,

© 2017 S. Irum et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
plants, Anthelmintics,