Determining the value of a half-effective or half-life concentration or dose of toxicant is the main purpose of acute toxicity studies, and this is also the most commonly used value in the toxicity characteristics of substances. By conducting tests that meet the criteria and requirements for the determination of acute toxicity, due to the use of appropriate mathematical tools and concentrations resulting in complete lethal effects in the studied groups, considerably more important values can be achieved, which give a possibility for the analysis of the entire process’s dynamics, as well as determining the threshold values of the effect time and toxicant concentration. This was the purpose of our research, in which the research species were