<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Biocatalysis is one of the most important industrial methods which has been increasingly attracting attention of scientists as a new and environmentally acceptable method. It is used in the preparation of chiral alcohols - important building blocks for the synthesis of fine chemicals, pharmaceuticals, agrochemicals and analogues of natural substances. In biocatalysis the use of traditional chiral agents based on heavy metals has been replaced by the use of enzymes. In many cases, the number of reaction steps has been limited, the selectivity of the desired products has been increased while the negative impact on the environment has been reduced. The principles of biocatalysis have been applied by us to design a simple chemical experiment for the students of higher secondary education. The modified assignment of a lab task consisted of bioreduction of 4-nitroacetophenone using the enzymes present in plant tissues of carrot, parsley and white radish. Within our pedagogical research, the adequacy of the chemical experiment for secondary school students was examined and the extent of understanding of the green chemistry experiment was analysed by the method of a semi-structured interview.</p></abstract>

Biocatalysis is one of the most important industrial methods which has been increasingly attracting attention of scientists as a new and environmentally acceptable method. It is used in the preparation of chiral alcohols - important building blocks for the synthesis of fine chemicals, pharmaceuticals, agrochemicals and analogues of natural substances. In biocatalysis the use of traditional chiral agents based on heavy metals has been replaced by the use of enzymes. In many cases, the number of reaction steps has been limited, the selectivity of the desired products has been increased while the negative impact on the environment has been reduced. The principles of biocatalysis have been applied by us to design a simple chemical experiment for the students of higher secondary education. The modified assignment of a lab task consisted of bioreduction of 4-nitroacetophenone using the enzymes present in plant tissues of carrot, parsley and white radish. Within our pedagogical research, the adequacy of the chemical experiment for secondary school students was examined and the extent of understanding of the green chemistry experiment was analysed by the method of a semi-structured interview.

Biocatalytic reduction of ketones in a secondary school laboratory

Department of Didactics in Science, Psychology and Pedagogy, Faculty of Natural Sciences

Chemistry-Didactics-Ecology-Metrology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Biocatalytic reduction of ketones in a secondary school laboratory"}

Biocatalysis is one of the most important industrial methods which has been increasingly attracting attention of scientists as a new and environmentally...

Biocatalytic reduction of ketones in a secondary school laboratory

Publié en ligne: 27 janv. 2018

Pages: 123 - 133

DOI: https://doi.org/10.1515/cdem-2017-0007

Mots clés
biocatalysis, green chemistry, enzymes, school experiment, semi-structured interview

© 2017 Dominika Lacušková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Biocatalytic reduction of ketones in a secondary school laboratory

Publié en ligne: 27 janv. 2018

Pages: 123 - 133

DOI: https://doi.org/10.1515/cdem-2017-0007

Mots clésbiocatalysis, green chemistry, enzymes, school experiment, semi-structured interview

© 2017 Dominika Lacušková et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
biocatalysis, green chemistry, enzymes, school experiment, semi-structured interview