<abstract xmlns="http://www.w3.org/1999/xhtml"><p> In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial- related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation.</p></abstract>

In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in oocyte maturation. A large number of mitochondrial DNA (mtDNA), copied in oocytes, is essential for providing adenosine triphosphate (ATP) during oocyte maturation. The purpose of this study was to identify the relationship between transcript expression levels of the mitochondrial encoded gene (MT-CO1) and two nuclear encoded genes, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM) in various stages of human oocyte maturation. Nine consenting patients, age 21-35 years old, with male factors were selected for ovarian stimulation and intracytoplasmic sperm injection (ICSI) procedures. mRNA levels of mitochondrial- related genes were performed by singlecell TaqMan® quantitative real-time polymerase chain reaction (qRT-PCR). There was no significant relationship between the relative expression levels in germinal vesicle (GV) stage oocytes (p = 0.62). On the contrary, a significant relationship was seen between the relative expression levels of TFAM and NRF1 and the MT-CO1 genes at the stages of metaphase I (MI) and MII (p = 0.03 and p = 0.002). A relationship exists between the transcript expression levels of TFAM and NRF1, and MT-CO1 genes in various stages of human oocyte maturation.

The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation

Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Science, Tehran, Iran

Department of Anatomical Sciences, Faculty of Medicine, Kurdistan University of Medical Sciences, Pasdaran Street, Sanandaj, Iran. Tel: +98-873-664-673. Fax: +98-873-364-674

Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Deptartment of Obstetrics and Gynecology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

In some cases of infertility in women, human oocytes fail to mature when they reach the metaphase II (MII) stage. Mitochondria plays an important role in...

The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation

Publié en ligne: 30 déc. 2015

Pages: 39 - 46

DOI: https://doi.org/10.1515/bjmg-2015-0004

Mots clés
Mitochondria, Oocyte maturation, Quantitative real-time polymerase chain reaction (qRT-PCR), single-cell

© Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The Relationship Between Transcript Expression Levels of Nuclear Encoded (TFAM, NRF1) and Mitochondrial Encoded (MT-CO1) Genes in Single Human Oocytes During Oocyte Maturation

Publié en ligne: 30 déc. 2015

Pages: 39 - 46

DOI: https://doi.org/10.1515/bjmg-2015-0004

Mots clésMitochondria, Oocyte maturation, Quantitative real-time polymerase chain reaction (qRT-PCR), single-cell

© Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Mitochondria, Oocyte maturation, Quantitative real-time polymerase chain reaction (qRT-PCR), single-cell