<abstract xmlns="http://www.w3.org/1999/xhtml"> The present study tested the susceptibility and pathological changes of catla, Catla catla (Hamilton) infected with Edwardsiella tarda (ET-PG-29). The bacterium was isolated from the kidney of a diseased pangas catfish. To determine the median lethal dose (LD50), C. catla were challenged with this bacterium (108-103 CFU ml-1), and the LD50 was calculated as 105.5 CFU ml-1. Another set of healthy C. catla were injected intraperitoneally with the LD50 dose to induce edwardsiellosis. The clinical signs of the infected C. catla were observed and recorded. Tissues such as kidney, liver, intestine, heart, and gill from the infected fish with clinical signs of edwardsiellosis were used for histopathology. The clinical and gross signs were first visible at 1 d post-injection, and the infected fish showed typical signs of hemorrhagic septicemia. The most striking histopathological features were found in the kidney which showed multi-focal necrosis with the formation of granuloma indicating an inflammatory response against the pathogen. The intestine displayed goblet cell hyperplasia, the liver showed hydropic degeneration with hyperemic central veins, and there was inflammation of gill lamellae and cardiac myositis associated with leucocyte infiltration. Collectively, the results confirmed the susceptibility of C. catla to E. tarda infection and that this bacterium is a threat to C. catla in aquaculture practices.</abstract>

The present study tested the susceptibility and pathological changes of catla, Catla catla (Hamilton) infected with Edwardsiella tarda (ET-PG-29). The bacterium was isolated from the kidney of a diseased pangas catfish. To determine the median lethal dose (LD50), C. catla were challenged with this bacterium (108-103 CFU ml-1), and the LD50 was calculated as 105.5 CFU ml-1. Another set of healthy C. catla were injected intraperitoneally with the LD50 dose to induce edwardsiellosis. The clinical signs of the infected C. catla were observed and recorded. Tissues such as kidney, liver, intestine, heart, and gill from the infected fish with clinical signs of edwardsiellosis were used for histopathology. The clinical and gross signs were first visible at 1 d post-injection, and the infected fish showed typical signs of hemorrhagic septicemia. The most striking histopathological features were found in the kidney which showed multi-focal necrosis with the formation of granuloma indicating an inflammatory response against the pathogen. The intestine displayed goblet cell hyperplasia, the liver showed hydropic degeneration with hyperemic central veins, and there was inflammation of gill lamellae and cardiac myositis associated with leucocyte infiltration. Collectively, the results confirmed the susceptibility of C. catla to E. tarda infection and that this bacterium is a threat to C. catla in aquaculture practices.

Susceptibility and pathological consequences of catla, Catla catla (Hamilton) experimentally infected with Edwardsiella tarda

Department of Fish Health and Environment College of Fisheries, Central Agricultural University Lembucherra, Tripura (w) – 799210, Tripura,

Department of Fishery Pathology and Microbiology, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences 5 – Budherhat Road, Chakgaria, P. O – Panchasayar Kolkata – 700094, West Bengal,

Fisheries & Aquatic Life

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The present study tested the susceptibility and pathological changes of catla, Catla catla (Hamilton) infected with Edwardsiella tarda (ET-PG-29). The...

Susceptibility and pathological consequences of catla, Catla catla (Hamilton) experimentally infected with Edwardsiella tarda

Article Category: Research Article

Publié en ligne: 09 févr. 2017

Pages: 209 - 217

Reçu: 30 janv. 2016

Accepté: 30 août 2016

DOI: https://doi.org/10.1515/aopf-2016-0018

Mots clés
Edwardsiellosis, Catla, pathology, granulomatous inflammation

© Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Susceptibility and pathological consequences of catla, Catla catla (Hamilton) experimentally infected with Edwardsiella tarda

Article Category: Research Article

Publié en ligne: 09 févr. 2017

Pages: 209 - 217

Reçu: 30 janv. 2016

Accepté: 30 août 2016

DOI: https://doi.org/10.1515/aopf-2016-0018

Mots clésEdwardsiellosis, Catla, pathology, granulomatous inflammation

© Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Edwardsiellosis, Catla, pathology, granulomatous inflammation