<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a universal primer of part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and goat. PCR product size was 512 bp. The PCR product of 16S rRNA was digested with two restriction enzymes (BclI and MseI); sufficient to easily generate analyzable species-specific restriction profiles that could distinguish the unambiguity of all targeted species. The HRM method successfully identified all species by shape of melting temperature, and proved to be of higher resolution, and a more cost effective, alternative method compared with other identification techniques.</p></abstract>

Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a universal primer of part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and goat. PCR product size was 512 bp. The PCR product of 16S rRNA was digested with two restriction enzymes (BclI and MseI); sufficient to easily generate analyzable species-specific restriction profiles that could distinguish the unambiguity of all targeted species. The HRM method successfully identified all species by shape of melting temperature, and proved to be of higher resolution, and a more cost effective, alternative method compared with other identification techniques.

8.Animal Species Identification through High Resolution Melting Real Time PCR (HRM) of the Mitochondrial 16S rRNA Gene

Department of Animal Science, Faculty of Agriculture

Research and Development Network Center for Animal Breeding (NCAB), Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a...

8.Animal Species Identification through High Resolution Melting Real Time PCR (HRM) of the Mitochondrial 16S rRNA Gene

Article Category: Animal Genetics and Breeding

Publié en ligne: 06 mai 2016

Pages: 415 - 424

Reçu: 12 mai 2015

Accepté: 27 oct. 2015

DOI: https://doi.org/10.1515/aoas-2015-0074

Mots clés
16S rRNA gene, HRM, PCR-RFLP, species identification

© by Monchai Duangjinda

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

8.Animal Species Identification through High Resolution Melting Real Time PCR (HRM) of the Mitochondrial 16S rRNA Gene

Article Category: Animal Genetics and Breeding

Publié en ligne: 06 mai 2016

Pages: 415 - 424

Reçu: 12 mai 2015

Accepté: 27 oct. 2015

DOI: https://doi.org/10.1515/aoas-2015-0074

Mots clés16S rRNA gene, HRM, PCR-RFLP, species identification

© by Monchai Duangjinda

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
16S rRNA gene, HRM, PCR-RFLP, species identification