<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This study aimed to evaluate contamination and stability in copper (Cu) analysis of serum and urine by flame atomic absorption spectroscopy (FAAS) and to compare the stability of urine Cu in controls and in D-penicillamine (D-PA) administration. Cu was measured by AAnalyst 400, Perkin Elmer, USA. Blood was collected in BD Vacutainer®SSTTM II Advance tubes and BD Vacutainer® Trace Element tubes. Sterile polyethylene and polypropylene vessels for collection, transportation, storage and preliminary preparation of samples were used in urinalysis. Stability in serum and 24 h urine was evaluated in two temperature regimens: 15-25°C and 2-8°C, for particular time of storage. No significant differences (p = 0.20) in Cu concentration was found between the two types of tested tubes with patient`s sera. The stability of the samples (serum and urine) was better at refrigeration temperature. In urine the stability was better in D-PA administration.Standardization of Cu analysis could be achieved by assessing the aspects of pre-analytical factors of variations.</p></abstract>

Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This study aimed to evaluate contamination and stability in copper (Cu) analysis of serum and urine by flame atomic absorption spectroscopy (FAAS) and to compare the stability of urine Cu in controls and in D-penicillamine (D-PA) administration. Cu was measured by AAnalyst 400, Perkin Elmer, USA. Blood was collected in BD Vacutainer®SSTTM II Advance tubes and BD Vacutainer® Trace Element tubes. Sterile polyethylene and polypropylene vessels for collection, transportation, storage and preliminary preparation of samples were used in urinalysis. Stability in serum and 24 h urine was evaluated in two temperature regimens: 15-25°C and 2-8°C, for particular time of storage. No significant differences (p = 0.20) in Cu concentration was found between the two types of tested tubes with patient`s sera. The stability of the samples (serum and urine) was better at refrigeration temperature. In urine the stability was better in D-PA administration.Standardization of Cu analysis could be achieved by assessing the aspects of pre-analytical factors of variations.

Serum and Urine Copper – Contamination and Stability

Clinical Laboratory Deparment, University Hospital Sv. Ivan Rilski, Medical University – Sofia , Bulgary Clinical Laboratory Department, University Hospital St. Ivan Rilski, Medical University, Acad. Ivan Geshov, 15 Blvd, Bg 1431 Sofia, Bulgary

Department of Clinical Laboratory and Immunology, University Hospital Alexandrovska, Medical University – Sofia, Bulgary

Clinic of Gastroenterology, University Hospital Sv. Ivan Rilski, Medical University – Sofia, Bulgary

Clinic of Nephrology, University Hospital Sv. Ivan Rilski, Medical University – Sofia, Bulgary

Clinical Laboratory Deparment, University Hospital Sv. Ivan Rilski, Medical University – Sofia

Acta Medica Bulgarica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Serum and Urine Copper \u2013 Contamination and Stability"}

Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This...

Serum and Urine Copper – Contamination and Stability

Publié en ligne: 26 nov. 2015

Pages: 49 - 60

DOI: https://doi.org/10.1515/amb-2015-0018

Mots clés
Cu, atomic absorption, pre-analytical phase, contamination, stability, Dpenicillamine

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Serum and Urine Copper – Contamination and Stability

Publié en ligne: 26 nov. 2015

Pages: 49 - 60

DOI: https://doi.org/10.1515/amb-2015-0018

Mots clésCu, atomic absorption, pre-analytical phase, contamination, stability, Dpenicillamine

© 2015

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Cu, atomic absorption, pre-analytical phase, contamination, stability, Dpenicillamine