<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.</p></abstract>

Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.

Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Institute for Soil Science, Teodora Drajzera 7, 11 000 Belgrade, Serbia

Institute of Veterinary Medicine, Boška Jovića 6, 35 000 Jagodina, Serbia

Faculty of Veterinary Medicine, Bulevar Oslobođenja 18, 11 000 Belgrade, Serbia

Military Medical Academy, Crnotravska 17, 11000 Belgrade, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD"}

Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human...

Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Publié en ligne: 28 juin 2016

Pages: 203 - 213

Reçu: 15 oct. 2015

Accepté: 06 avr. 2016

DOI: https://doi.org/10.1515/acve-2016-0017

Mots clés
broilers, Campylobacter spp., epidemiology, multiplex-PCR, RAPD

© by Jelena Petković

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Typing of Indigenous Campylobacter spp. From Serbia by M-PCR and RAPD

Publié en ligne: 28 juin 2016

Pages: 203 - 213

Reçu: 15 oct. 2015

Accepté: 06 avr. 2016

DOI: https://doi.org/10.1515/acve-2016-0017

Mots clésbroilers, Campylobacter spp., epidemiology, multiplex-PCR, RAPD

© by Jelena Petković

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
broilers, Campylobacter spp., epidemiology, multiplex-PCR, RAPD