<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Resistance to tetracycline was studied in <italic>Salmonella</italic> Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that <italic>Salmonella</italic> Infantis had also spread to breeders and layers. In this work, we detected the <italic>tetA</italic> gene and the corresponding <italic>tetR</italic> gene (encoding the repressor protein) as well as the truncated transposon Tn<italic>1721,</italic> which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in <italic>Salmonella</italic>. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by <italic>Salmonella</italic> spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of <italic>Salmonella</italic> Infantis resistant clones.</p></abstract>

Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.

Characterization Of Tetracycline Resistance Of <italic>Salmonella Enterica</italic> Subspecies <italic>Enterica</italic> Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia

Scientific Veterinary Institute „Novi Sad“, Novi Sad, Republic of Serbia

Veterinary Institute Kraljevo, Kraljevo, Republic of Serbia

Faculty of Medicine, Department of Pharmacy, University of Novi Sad, Republic of Serbia

Faculty of Veterinary Medicine Belgrade, University of Belgrade, Republic of Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Characterization Of Tetracycline Resistance Of  Subspecies  Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia

Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of...

Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia

Publié en ligne: 22 déc. 2015

Pages: 548 - 556

Reçu: 16 juil. 2015

Accepté: 13 nov. 2015

DOI: https://doi.org/10.1515/acve-2015-0046

Mots clés
poultry, Infantis, tetracycline, gene, gene, transposone Tn

© 2015 Todorović Dalibor et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Characterization Of Tetracycline Resistance Of Salmonella Enterica Subspecies Enterica Serovar Infantis Isolated From Poultry In The Northern Part Of Serbia

Publié en ligne: 22 déc. 2015

Pages: 548 - 556

Reçu: 16 juil. 2015

Accepté: 13 nov. 2015

DOI: https://doi.org/10.1515/acve-2015-0046

Mots cléspoultry, Infantis, tetracycline, gene, gene, transposone Tn

© 2015 Todorović Dalibor et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
poultry, Infantis, tetracycline, gene, gene, transposone Tn