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2603-347X
Publicado por primera vez
15 Dec 2015
Periodo de publicación
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Volumen 7 (2020): Edición 1 (March 2020)

Detalles de la revista
Formato
Revista
eISSN
2603-347X
Publicado por primera vez
15 Dec 2015
Periodo de publicación
1 tiempo por año
Idiomas
Inglés

Buscar

14 Artículos
Acceso abierto

Editorial Note: Natural Sciences - where Science meets Beauty

Publicado en línea: 18 Apr 2020
Páginas: I - III

Resumen

Acceso abierto

Fe-Chelating Activity of Conocarpus lancifolius Engl. Leaf Extracts and 1H-NMR Based Metabolite’s classification

Publicado en línea: 18 Apr 2020
Páginas: 1 - 7

Resumen

Abstract

The purpose of the work was to evaluate the Fe-chelating activity of freeze drying assisted ultrasonicated hydroethanolic leaf extracts of Conocarpus lancifolius Engl. along with 1H-NMR based classification of metabolites in most active extract. The finding revealed that 60% ethanolic extract was the most active fraction regarding Fe-chelating activity with value of 75.4 ± 0.6% followed by 80% ethanolic extract having chelating value of 69.24±1.02%. The least Fe-chelating activity was exhibited by aqueous extract. The statistical analysis revealed that the Fe-chelating activity by 60% ethanolic extract was significantly higher than other fractions except EDTA which was used as standard chelating agent. The 1HNMR technique predicted the presence of aromatic secondary metabolites of polyphenolic origin due to numerous peaks in respective regions. The peaks in carbohydrate and organic acid regions were also observed. The research outcomes suggested that C. lancifolius may be workable choice to move further for the development of cure and management practices for iron load based oxidative stress oriented diseases including diabetes mellitus type II.

Palabras clave

  • ultrasonication
  • Fe-chelating activity
  • H-NMR
  • metabolite prediction
Acceso abierto

Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro

Publicado en línea: 18 Apr 2020
Páginas: 8 - 25

Resumen

Abstract

This work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its media and under a 16h/8h photoperiod except for the diameter of the tubers, where this genotype had the best diameter over the medium (MS/2+KIN) under an 8h photoperiod.

Palabras clave

  • MS/2 medium
  • microtuberization
  • BAP
  • kinetin
  • L
Acceso abierto

Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

Publicado en línea: 18 Apr 2020
Páginas: 26 - 32

Resumen

Abstract

The aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

Palabras clave

  • essential oil
  • antibiogramme
  • antibacterial activity
Acceso abierto

In vitro antioxidant activity and protective effect of Hertia cheirifolia L. n-butanol extract against liver and heart mitochondrial oxidative stress in rat

Publicado en línea: 18 Apr 2020
Páginas: 33 - 45

Resumen

Abstract

The current study investigated the protective effect of Hertia cheirifolia L. n-butanol extract on oxidative stress in vitro by measuring lipid peroxidation (MDA) level and superoxide anion (O2•-) production in liver and heart mitochondria of rat. In addition, the antioxidant potential of H. cheirifolia n-butanol extract was evaluated by using five methods: ABTS•+, O2•-, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. The results indicated that n-butanol extract contained large amounts of total phenolics (203.52±1.81 mg GAE/g) and flavonoids (104.86±0.57 mg QE/g), and had an interesting antioxidant activity and protective effect on mitochondrial oxidative stress. Therefore, H. cheirifolia n-butanol extract may serve as potential source of natural antioxidant for pharmaceutical applications.

Palabras clave

  • L.
  • phenolics
  • flavonoids
  • liver and heart mitochondria
  • antioxidant activity
Acceso abierto

In vitro investigation of the antioxidant and antimicrobial effects of hydro-alcoholic and aqueous extracts of Globularia alypum L.

Publicado en línea: 18 Apr 2020
Páginas: 46 - 58

Resumen

Abstract

The aim of this work was to establish the antioxidant and antimicrobial activity of hydro-alcoholic and aqueous extracts prepared from the leaves of Globularia alypum L. The quantitative estimation of total polyphenols (by the Folin-Ciocalteu method) and total flavonoids (by the method of aluminium trichloride) showed that the aqueous and ethyl acetate extracts had the highest content of phenolic and flavonoid compounds. Moreover, the results of antioxidant power assessed by both method DPPH and phosphomolybdenum indicated that leaves extracts of G. alypum expressed a considerable activity. The evaluation of the antimicrobial effects, using the disc diffusion method from antibacterial screening and the direct contact method from the antifungal activity, indicated strong antibacterial and antifungal activity.

Palabras clave

  • L.
  • phenolic extracts
  • antimicrobial activity
  • antioxidant activity
Acceso abierto

Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

Publicado en línea: 18 Apr 2020
Páginas: 59 - 70

Resumen

Abstract

The chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10.95%). The phytochemical study led to the isolation and structural elucidation of three compounds, scopoletin, umckalin and 1-(3’,4’-dihydroxycinnamoyl) cyclopentane-2,3-diol. Moreover the ethyl acetate extract was screened for its possible in vitro antioxidant activity by 2,2-diphenyl-1-picrylhydrazy l(DPPH) and lipid peroxidation inhibition assays in which it displayed a noticeable activity. This study provides the first biological and chemical investigation on Anthriscus vulgaris Bernh. in Algeria.

Palabras clave

  • Bernh.
  • GC-MS
  • scopoletin
  • umckalin
  • DPPH
Acceso abierto

Antimicrobial activity of aqueous methanolic extracts of Algerian cultivars of sorghum (Sorghum bicolor (L.) Moench)

Publicado en línea: 18 Apr 2020
Páginas: 71 - 85

Resumen

Abstract

Certain phenolics have been recognized to possessing antibacterial and antifungal activities and high levels of flavonoids and tannins have been reported in several varieties of Sorghum bicolor (L.) Moench. Antimicrobial activity and phenolics contents were investigated in five Algerian sorghum seeds. AS20 sorghum extract showed the highest levels of: total phenolics (3214.46±263.64 mg/100 g), flavonoids (32.03±1.64 mg/100 g) and tannins (615.35±6.10 mg/g) contents; however, comparable flavonoids content was recorded in I27 extract. FZ40 and AS12 flavonoids contents were comparable. Screening for antimicrobial activity, carried out by the disc’s diffusion method revealed an antimicrobial potential of sorghum crude extracts against Gram-positive and Gram-negative bacteria and candida albicans yeast. Minimal inhibition concentration determined by microdilution method varied between 0.2 and 2 mg/ml. the lowest value was recorded with F11 and FZ40 extract against Streptococcus pneumoniae and F11 against Escherichia coli. Bacillus subtilis, Staphylococcus aureus ATTCC6538 and MRSA strains showed sensitivity to all extracts. The results show these sorghums as a potential source of natural anti-streptococcal, anti-staphylococcal and anti-candida substances.

Palabras clave

  • (L.) Moench.
  • phenolics
  • flavonoids
  • tannins
  • antimicrobial activity
Acceso abierto

Allelopathic activity of essential oil extracts from Artemisia herba-alba Asso. on seed and seedling germination of weed and wheat crops

Publicado en línea: 18 Apr 2020
Páginas: 86 - 97

Resumen

Abstract

The allelopathic potential of essential oil extracts from Artemisia herba-alba Asso. was assessed on seed germination of nine weeds and two wheat varieties. The samples were collected from El-Rasfa région (Sétif, Algeria). The essential oil was extracted using hydrodistillation of aerial part and characterized using gaz chromatography coupled with mass spectroscopy. The bioassays with organic solvent (ethanol) were tested using four different concentrations (0.2, 0.4, 0.6 and 0.8 µl/ml of oil/ethanol) on seed and seedling growth of the nine weeds and two wheat varieties. The yield obtained is 1.19%, and 36 compound had been identified. The main components are: camphor (28.58%), cis-thujone (22.03%), eucalyptol (11.65%) and trans-thujone (7.03%). The results of bioassays show that essential oil extracts has a significant effect on seed germination and seedling growth of the major weed tested and two wheat varieties. In conclusion, this study shows that the essential oil tested has an interesting allelopathic potential.

Palabras clave

  • allelopathy
  • Asso.
  • essential oil
  • weeds
  • wheat
Acceso abierto

Analysis of the main agronomic characters of some barley varieties and the genetic characterization of their descendancy after a full diallel cross

Publicado en línea: 18 Apr 2020
Páginas: 98 - 111

Resumen

Abstract

The experiment was conducted at the level of a pilot farm located in eastern Algeria under a humid bioclimatic stage, during two successive crop years. The study focused on a F1 generation of barley (Hordeum vulgare L.) consisting of twelve hybrids from a complete diallel cross between the two locals varieties (Saida and Tichedrett) and two other introduced varieties (Nadawa and Fouara). The aim was to determine the value of parental genotypes as genitors and to analyze their descendants, while evaluating the phenotypic variability of ten quantitative variables. Analysis of the variance revealed a significant difference for the whole of parameters studied in the parents as in their descendants. Additive and non-additive effects are involved in the genetic control of the analyzed variables. The Hayman model (1954) seems acceptable for five variables on ten variables studied for which additive effects are more important than dominance effects. The analysis of the heterosis effect was significant for the characters tested. For the productivity of the plant, eight hybrids on twelve have expressed a positive heterosis compared to the mid- parent, six combinations on twelve have registered a positive heterosis compared to the over-batter parent and compared to the best variety with an overall heterosis of 17.53%.

Palabras clave

  • barley
  • complete diallel
  • Hayman model
  • heterosis effect
Acceso abierto

The anti-acetylcholinesterase, insecticidal and antifungal activities of the entophytic fungus Trichoderma sp. isolated from Ricinus communis L. against Locusta migratoria L. and Botrytis cinerea Pers.: Fr.

Publicado en línea: 18 Apr 2020
Páginas: 112 - 125

Resumen

Abstract

This study was conducted to evaluate anti-acetylcholinesterase and insecticidal and antifungal activities of the endophytic fungus Trichoderma sp, isolated from Ricinus communis L. leaves, against Locusta migratoria L. and Botrytis cinerea Pers.: Fr.. To evaluate the insecticidal and antifungal activities, different concentrations of the fungal extract were applied against L. migratoria (0.2, 0.3, 0.4 g/l) and against B. cinerea (1, 2, 3 g/l). It was found that the mortality of the targeted insects was positively proportional to fungal extract concentration and time after exposure (24, 48, 72 hours). The concentration 0.4 g/l appeared to be the most effective after 72 hours with mortality rate of 56.52%. Regarding antifungal activity, the concentration 3 g/l was the most effective against B. cinerea after 7 days, with an inhibition rate of 92.06% (excellent antifungal activity). Moreover, it was found that at 4 ug/ml the fungal extract had a maximum inhibitory capacity of Ache of 80% for acetylcholenesterase. Preliminary phytochemical analyses revealed the presence of alkaloids, flavonoids, phenols and saponins. In addition the colony of this endophytic fungus produced chitinases and proteases, which explained its important antifungal and insecticidal activities.

Palabras clave

  • insecticidal activity
  • antifungal activity
  • endophytic fungi
Acceso abierto

The insecticidal activity of the endophytic fungus Isaria fumosorosea Wize isolated from the leaves of the Ricinus communis L. against Locusta migratoria L. and Acanthoscelides obtectus Say

Publicado en línea: 18 Apr 2020
Páginas: 126 - 135

Resumen

Abstract

This study was conducted to evaluate the insecticidal activity of the endophytic fungus Isaria fumosorosea Wize isolated from Ricinus communis L. against Locusta migratoria L. and Acanthoscelides obtectus Say. To evaluate the insecticidal activity, a mixture of fungal extract and spore suspensions (1x107, 1x106 and 1x105 spores/ml of dissolved extract) were applied against the insects. It was found that I. fumosorosea had a good insecticidal activity, positively proportional to concentration and time after exposure (24, 48, 72 hours). A maximum mortality rate of 80% for A. obtectus and 70% for L. migratoria at 1x107 spores/ml of dissolved extract was recorded. Preliminary phytochemical analyses of the fungal extract revealed the presence of alkaloids, flavonoids and phenols. Additionally, a fungal development on the insects bodies has also been noticed; that is undoubtedly explained the recorded insecticidal activity.

Palabras clave

  • insecticidal activity
  • endophytic fungi
  • Ricinus communis L.
  • Isaria fumosorosea Wize
  • Locusta migratoria L.
  • Acanthoscelides obtectus Say
Acceso abierto

Second Version of SpectraView for Spectra Analysis

Publicado en línea: 18 Apr 2020
Páginas: 136 - 139

Resumen

Abstract

This short communication presents a second version of the program SpectraView (SV2). The Second Version of SpectraView for Spectra Analysis is based on data collected with 2-m RCC telescope at Rozhen National Astronomical Observatory. This version is equipped with new user interface and other features unavailable in its previous versions. It has also been created using modern programming language and is now compatible with the latest operating systems. The program has been tested on the observations of the five short-period variable stars.

Palabras clave

  • Variable stars
  • Spectra
  • Programs
Acceso abierto

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe3o4)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Publicado en línea: 06 May 2020
Páginas: 140 - 154

Resumen

Abstract

During recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause cellular death by inducing oxidative stress. Present study aimed to investigate whether diferuloylmethane could protect rats mitochondria against Fe3O4-NPs intoxication. Twenty adult male rats were randomly chosen and divided into four groups: control; treated with 10 mg/kg/d of Fe3O4-NPs; treated with diferuloylmethane at the dose 20 ml/kg/d; treated with Fe3O4-NPs (10 mg/kg/d) and diferuloylmethane (20 ml/kg/d) respectively for 28 days. The results showed that Fe3O4-NPs increased the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation, mit-GSH (Glutathione), mit-CAT (Catalase), mit-GST (Glutathione S-transferase) and decreased mit-GPx (Glutathione peroxidase), with increased in mitochondrial swelling and permeability followed by the increasing level of plasmatic Cyt-c. The addition of diferuloylmethane (DFM) to these samples reduces or corrects the amount of the most of biomarkers. These findings have demonstrated that DFM can act as an antioxidant and antiapoptotic factor against damages induced by Fe3O4-NPs.

Palabras clave

  • mitochondrial status
  • oxidative stress
  • Cyt-c
  • apoptosis
  • diferuloylmethane
14 Artículos
Acceso abierto

Editorial Note: Natural Sciences - where Science meets Beauty

Publicado en línea: 18 Apr 2020
Páginas: I - III

Resumen

Acceso abierto

Fe-Chelating Activity of Conocarpus lancifolius Engl. Leaf Extracts and 1H-NMR Based Metabolite’s classification

Publicado en línea: 18 Apr 2020
Páginas: 1 - 7

Resumen

Abstract

The purpose of the work was to evaluate the Fe-chelating activity of freeze drying assisted ultrasonicated hydroethanolic leaf extracts of Conocarpus lancifolius Engl. along with 1H-NMR based classification of metabolites in most active extract. The finding revealed that 60% ethanolic extract was the most active fraction regarding Fe-chelating activity with value of 75.4 ± 0.6% followed by 80% ethanolic extract having chelating value of 69.24±1.02%. The least Fe-chelating activity was exhibited by aqueous extract. The statistical analysis revealed that the Fe-chelating activity by 60% ethanolic extract was significantly higher than other fractions except EDTA which was used as standard chelating agent. The 1HNMR technique predicted the presence of aromatic secondary metabolites of polyphenolic origin due to numerous peaks in respective regions. The peaks in carbohydrate and organic acid regions were also observed. The research outcomes suggested that C. lancifolius may be workable choice to move further for the development of cure and management practices for iron load based oxidative stress oriented diseases including diabetes mellitus type II.

Palabras clave

  • ultrasonication
  • Fe-chelating activity
  • H-NMR
  • metabolite prediction
Acceso abierto

Interactive effect of genotype and medium on microtuberization of potatoes (Solanum tuberosum L.) grown in vitro

Publicado en línea: 18 Apr 2020
Páginas: 8 - 25

Resumen

Abstract

This work investigates the interactive effect of six culture media and three photoperiods (darkness, 16h/8h, 8h/16h) on the microtuberization of four potato varieties (Spunta, Désirée, Kondor and Bartina). The objective is to determine the best tuberization under these growing conditions. The measured parameters which are related to the suitability of the tuberization characteristics are: the morphological aspects (shape, position) and the biometric ones (number and diameter of tubers). The obtained results permit to valorize the meristems that constitute the starting explant, and their good organogenetic skills to provide the first generation of micropropagation with healthy vitroplants in a sufficient quantity. Thus, the grown vitroplants on the medium (MS/2+BAP+COU) presented the best values which are related to the percentage of tuberization, the number of microtubercles / vitroplants and weight of tubers. Moreover, the Bartina genotype showed a remarkable superiority over its media and under a 16h/8h photoperiod except for the diameter of the tubers, where this genotype had the best diameter over the medium (MS/2+KIN) under an 8h photoperiod.

Palabras clave

  • MS/2 medium
  • microtuberization
  • BAP
  • kinetin
  • L
Acceso abierto

Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

Publicado en línea: 18 Apr 2020
Páginas: 26 - 32

Resumen

Abstract

The aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

Palabras clave

  • essential oil
  • antibiogramme
  • antibacterial activity
Acceso abierto

In vitro antioxidant activity and protective effect of Hertia cheirifolia L. n-butanol extract against liver and heart mitochondrial oxidative stress in rat

Publicado en línea: 18 Apr 2020
Páginas: 33 - 45

Resumen

Abstract

The current study investigated the protective effect of Hertia cheirifolia L. n-butanol extract on oxidative stress in vitro by measuring lipid peroxidation (MDA) level and superoxide anion (O2•-) production in liver and heart mitochondria of rat. In addition, the antioxidant potential of H. cheirifolia n-butanol extract was evaluated by using five methods: ABTS•+, O2•-, Bleaching of β-carotene in linoleic acid, CUPRAC and Ferric reducing power. The results indicated that n-butanol extract contained large amounts of total phenolics (203.52±1.81 mg GAE/g) and flavonoids (104.86±0.57 mg QE/g), and had an interesting antioxidant activity and protective effect on mitochondrial oxidative stress. Therefore, H. cheirifolia n-butanol extract may serve as potential source of natural antioxidant for pharmaceutical applications.

Palabras clave

  • L.
  • phenolics
  • flavonoids
  • liver and heart mitochondria
  • antioxidant activity
Acceso abierto

In vitro investigation of the antioxidant and antimicrobial effects of hydro-alcoholic and aqueous extracts of Globularia alypum L.

Publicado en línea: 18 Apr 2020
Páginas: 46 - 58

Resumen

Abstract

The aim of this work was to establish the antioxidant and antimicrobial activity of hydro-alcoholic and aqueous extracts prepared from the leaves of Globularia alypum L. The quantitative estimation of total polyphenols (by the Folin-Ciocalteu method) and total flavonoids (by the method of aluminium trichloride) showed that the aqueous and ethyl acetate extracts had the highest content of phenolic and flavonoid compounds. Moreover, the results of antioxidant power assessed by both method DPPH and phosphomolybdenum indicated that leaves extracts of G. alypum expressed a considerable activity. The evaluation of the antimicrobial effects, using the disc diffusion method from antibacterial screening and the direct contact method from the antifungal activity, indicated strong antibacterial and antifungal activity.

Palabras clave

  • L.
  • phenolic extracts
  • antimicrobial activity
  • antioxidant activity
Acceso abierto

Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

Publicado en línea: 18 Apr 2020
Páginas: 59 - 70

Resumen

Abstract

The chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10.95%). The phytochemical study led to the isolation and structural elucidation of three compounds, scopoletin, umckalin and 1-(3’,4’-dihydroxycinnamoyl) cyclopentane-2,3-diol. Moreover the ethyl acetate extract was screened for its possible in vitro antioxidant activity by 2,2-diphenyl-1-picrylhydrazy l(DPPH) and lipid peroxidation inhibition assays in which it displayed a noticeable activity. This study provides the first biological and chemical investigation on Anthriscus vulgaris Bernh. in Algeria.

Palabras clave

  • Bernh.
  • GC-MS
  • scopoletin
  • umckalin
  • DPPH
Acceso abierto

Antimicrobial activity of aqueous methanolic extracts of Algerian cultivars of sorghum (Sorghum bicolor (L.) Moench)

Publicado en línea: 18 Apr 2020
Páginas: 71 - 85

Resumen

Abstract

Certain phenolics have been recognized to possessing antibacterial and antifungal activities and high levels of flavonoids and tannins have been reported in several varieties of Sorghum bicolor (L.) Moench. Antimicrobial activity and phenolics contents were investigated in five Algerian sorghum seeds. AS20 sorghum extract showed the highest levels of: total phenolics (3214.46±263.64 mg/100 g), flavonoids (32.03±1.64 mg/100 g) and tannins (615.35±6.10 mg/g) contents; however, comparable flavonoids content was recorded in I27 extract. FZ40 and AS12 flavonoids contents were comparable. Screening for antimicrobial activity, carried out by the disc’s diffusion method revealed an antimicrobial potential of sorghum crude extracts against Gram-positive and Gram-negative bacteria and candida albicans yeast. Minimal inhibition concentration determined by microdilution method varied between 0.2 and 2 mg/ml. the lowest value was recorded with F11 and FZ40 extract against Streptococcus pneumoniae and F11 against Escherichia coli. Bacillus subtilis, Staphylococcus aureus ATTCC6538 and MRSA strains showed sensitivity to all extracts. The results show these sorghums as a potential source of natural anti-streptococcal, anti-staphylococcal and anti-candida substances.

Palabras clave

  • (L.) Moench.
  • phenolics
  • flavonoids
  • tannins
  • antimicrobial activity
Acceso abierto

Allelopathic activity of essential oil extracts from Artemisia herba-alba Asso. on seed and seedling germination of weed and wheat crops

Publicado en línea: 18 Apr 2020
Páginas: 86 - 97

Resumen

Abstract

The allelopathic potential of essential oil extracts from Artemisia herba-alba Asso. was assessed on seed germination of nine weeds and two wheat varieties. The samples were collected from El-Rasfa région (Sétif, Algeria). The essential oil was extracted using hydrodistillation of aerial part and characterized using gaz chromatography coupled with mass spectroscopy. The bioassays with organic solvent (ethanol) were tested using four different concentrations (0.2, 0.4, 0.6 and 0.8 µl/ml of oil/ethanol) on seed and seedling growth of the nine weeds and two wheat varieties. The yield obtained is 1.19%, and 36 compound had been identified. The main components are: camphor (28.58%), cis-thujone (22.03%), eucalyptol (11.65%) and trans-thujone (7.03%). The results of bioassays show that essential oil extracts has a significant effect on seed germination and seedling growth of the major weed tested and two wheat varieties. In conclusion, this study shows that the essential oil tested has an interesting allelopathic potential.

Palabras clave

  • allelopathy
  • Asso.
  • essential oil
  • weeds
  • wheat
Acceso abierto

Analysis of the main agronomic characters of some barley varieties and the genetic characterization of their descendancy after a full diallel cross

Publicado en línea: 18 Apr 2020
Páginas: 98 - 111

Resumen

Abstract

The experiment was conducted at the level of a pilot farm located in eastern Algeria under a humid bioclimatic stage, during two successive crop years. The study focused on a F1 generation of barley (Hordeum vulgare L.) consisting of twelve hybrids from a complete diallel cross between the two locals varieties (Saida and Tichedrett) and two other introduced varieties (Nadawa and Fouara). The aim was to determine the value of parental genotypes as genitors and to analyze their descendants, while evaluating the phenotypic variability of ten quantitative variables. Analysis of the variance revealed a significant difference for the whole of parameters studied in the parents as in their descendants. Additive and non-additive effects are involved in the genetic control of the analyzed variables. The Hayman model (1954) seems acceptable for five variables on ten variables studied for which additive effects are more important than dominance effects. The analysis of the heterosis effect was significant for the characters tested. For the productivity of the plant, eight hybrids on twelve have expressed a positive heterosis compared to the mid- parent, six combinations on twelve have registered a positive heterosis compared to the over-batter parent and compared to the best variety with an overall heterosis of 17.53%.

Palabras clave

  • barley
  • complete diallel
  • Hayman model
  • heterosis effect
Acceso abierto

The anti-acetylcholinesterase, insecticidal and antifungal activities of the entophytic fungus Trichoderma sp. isolated from Ricinus communis L. against Locusta migratoria L. and Botrytis cinerea Pers.: Fr.

Publicado en línea: 18 Apr 2020
Páginas: 112 - 125

Resumen

Abstract

This study was conducted to evaluate anti-acetylcholinesterase and insecticidal and antifungal activities of the endophytic fungus Trichoderma sp, isolated from Ricinus communis L. leaves, against Locusta migratoria L. and Botrytis cinerea Pers.: Fr.. To evaluate the insecticidal and antifungal activities, different concentrations of the fungal extract were applied against L. migratoria (0.2, 0.3, 0.4 g/l) and against B. cinerea (1, 2, 3 g/l). It was found that the mortality of the targeted insects was positively proportional to fungal extract concentration and time after exposure (24, 48, 72 hours). The concentration 0.4 g/l appeared to be the most effective after 72 hours with mortality rate of 56.52%. Regarding antifungal activity, the concentration 3 g/l was the most effective against B. cinerea after 7 days, with an inhibition rate of 92.06% (excellent antifungal activity). Moreover, it was found that at 4 ug/ml the fungal extract had a maximum inhibitory capacity of Ache of 80% for acetylcholenesterase. Preliminary phytochemical analyses revealed the presence of alkaloids, flavonoids, phenols and saponins. In addition the colony of this endophytic fungus produced chitinases and proteases, which explained its important antifungal and insecticidal activities.

Palabras clave

  • insecticidal activity
  • antifungal activity
  • endophytic fungi
Acceso abierto

The insecticidal activity of the endophytic fungus Isaria fumosorosea Wize isolated from the leaves of the Ricinus communis L. against Locusta migratoria L. and Acanthoscelides obtectus Say

Publicado en línea: 18 Apr 2020
Páginas: 126 - 135

Resumen

Abstract

This study was conducted to evaluate the insecticidal activity of the endophytic fungus Isaria fumosorosea Wize isolated from Ricinus communis L. against Locusta migratoria L. and Acanthoscelides obtectus Say. To evaluate the insecticidal activity, a mixture of fungal extract and spore suspensions (1x107, 1x106 and 1x105 spores/ml of dissolved extract) were applied against the insects. It was found that I. fumosorosea had a good insecticidal activity, positively proportional to concentration and time after exposure (24, 48, 72 hours). A maximum mortality rate of 80% for A. obtectus and 70% for L. migratoria at 1x107 spores/ml of dissolved extract was recorded. Preliminary phytochemical analyses of the fungal extract revealed the presence of alkaloids, flavonoids and phenols. Additionally, a fungal development on the insects bodies has also been noticed; that is undoubtedly explained the recorded insecticidal activity.

Palabras clave

  • insecticidal activity
  • endophytic fungi
  • Ricinus communis L.
  • Isaria fumosorosea Wize
  • Locusta migratoria L.
  • Acanthoscelides obtectus Say
Acceso abierto

Second Version of SpectraView for Spectra Analysis

Publicado en línea: 18 Apr 2020
Páginas: 136 - 139

Resumen

Abstract

This short communication presents a second version of the program SpectraView (SV2). The Second Version of SpectraView for Spectra Analysis is based on data collected with 2-m RCC telescope at Rozhen National Astronomical Observatory. This version is equipped with new user interface and other features unavailable in its previous versions. It has also been created using modern programming language and is now compatible with the latest operating systems. The program has been tested on the observations of the five short-period variable stars.

Palabras clave

  • Variable stars
  • Spectra
  • Programs
Acceso abierto

Cellular Apoptosis, Mitochondrial Swelling, Permeability and Cytochrome-C Level After (Fe3o4)-Nps Nanoparticles Exposure and Protective Role of Diferuloylmethane in Rats Liver

Publicado en línea: 06 May 2020
Páginas: 140 - 154

Resumen

Abstract

During recent years the defensive role of diferuloylmethane against oxidative stress and apoptosis has been experimentally documented. Fe3O4-NPs can cause cellular death by inducing oxidative stress. Present study aimed to investigate whether diferuloylmethane could protect rats mitochondria against Fe3O4-NPs intoxication. Twenty adult male rats were randomly chosen and divided into four groups: control; treated with 10 mg/kg/d of Fe3O4-NPs; treated with diferuloylmethane at the dose 20 ml/kg/d; treated with Fe3O4-NPs (10 mg/kg/d) and diferuloylmethane (20 ml/kg/d) respectively for 28 days. The results showed that Fe3O4-NPs increased the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid peroxidation, mit-GSH (Glutathione), mit-CAT (Catalase), mit-GST (Glutathione S-transferase) and decreased mit-GPx (Glutathione peroxidase), with increased in mitochondrial swelling and permeability followed by the increasing level of plasmatic Cyt-c. The addition of diferuloylmethane (DFM) to these samples reduces or corrects the amount of the most of biomarkers. These findings have demonstrated that DFM can act as an antioxidant and antiapoptotic factor against damages induced by Fe3O4-NPs.

Palabras clave

  • mitochondrial status
  • oxidative stress
  • Cyt-c
  • apoptosis
  • diferuloylmethane

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