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<p>The authors studied the <italic>in vitro</italic> antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecylpyridyl)porphine (C14) against four multidrug-resistant bacteria: <italic>Staphylococcus aureus</italic>, <italic>Enterococcus faecalis</italic> (Gram-positive), <italic>Escherichia coli, Pseudomonas aeruginosa</italic> (Gram-negative). Using 10 μg/ml of porphyrin and <italic>60 sec</italic> irradiation we observed the remarkable susceptibility of <italic>S. aureus</italic> and <italic>E. faecalis</italic> to treatment while, under the same conditions, <italic>E. coli</italic> and <italic>P. aeruginosa</italic> showed very low susceptibility. In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effective <italic>in vivo</italic> also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.</p>
</abstract>

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecylpyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility. In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effective in vivo also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecylpyridyl)porphine (C14)...

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Publicado en línea: 04 dic 2017

Páginas: 533 - 536

Recibido: 21 nov 2016

Aceptado: 12 ene 2017

DOI: https://doi.org/10.5604/pjm-2017-533

Palabras clave
antibiotic-resistant bacteria, laser light irradiation, porphyrin, photo-activation treatment

© 2017 MORENO BONDI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Publicado en línea: 04 dic 2017

Páginas: 533 - 536

Recibido: 21 nov 2016

Aceptado: 12 ene 2017

DOI: https://doi.org/10.5604/pjm-2017-533

Palabras claveantibiotic-resistant bacteria, laser light irradiation, porphyrin, photo-activation treatment

© 2017 MORENO BONDI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
antibiotic-resistant bacteria, laser light irradiation, porphyrin, photo-activation treatment