<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Klebsiella pneumoniae</italic> strains that are commonly recognized by clinicians and microbiologists are termed as classical <italic>K. pneumoniae</italic> (c<italic>KP</italic>). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent <italic>K. pneumoniae</italic> (hv<italic>KP</italic>) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated c<italic>KP</italic> and hv<italic>KP</italic> from respiratory tract infected patients. A total of 96 <italic>K. pneumoniae</italic> strains were isolated from sputum of respiratory tract infected patients. The isolates were performed string test, AST, ESBL virulence gene, serum bactericidal and biofilm assays. Out of 96 isolates, 39 isolates (40.6%) were identified with hypervirulent phenotypes. The number of c<italic>KP</italic> exhibiting resistance to the tested antimicrobials and ESBLs were significantly higher than that of the hv<italic>KP</italic> strains. The virulence genes of <italic>K. pneumoniae</italic> such as K1, K2, <italic>rmp</italic>A, <italic>uge</italic>, <italic>kfu</italic> and aerobactin were strongly associated with hv<italic>KP</italic> than c<italic>KP</italic>. However, no significant difference was found in FIM-1 and MrKD3 genes. ESBL producing c<italic>KP</italic> and hv<italic>KP</italic> were significantly associated with strong biofilm formation (both P &lt; 0.05) and highly associated with bactericidal effect of serum (both P &lt; 0.05) than c<italic>KP</italic> strains. However, neither biofilm formation nor bactericidal effect of serum was found with significant difference in between ESBL producing c<italic>KP</italic> and ESBL producing hv<italic>KP</italic> strains (both P > 0.05). Although the hv<italic>KP</italic> possess more virulence gene, but they didn’t show any significant difference between biofilm formation and bactericidal effect of serum compared with ESBL producing c<italic>KP</italic> strains.</p>
</abstract>

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients. The isolates were performed string test, AST, ESBL virulence gene, serum bactericidal and biofilm assays. Out of 96 isolates, 39 isolates (40.6%) were identified with hypervirulent phenotypes. The number of cKP exhibiting resistance to the tested antimicrobials and ESBLs were significantly higher than that of the hvKP strains. The virulence genes of K. pneumoniae such as K1, K2, rmpA, uge, kfu and aerobactin were strongly associated with hvKP than cKP. However, no significant difference was found in FIM-1 and MrKD3 genes. ESBL producing cKP and hvKP were significantly associated with strong biofilm formation (both P < 0.05) and highly associated with bactericidal effect of serum (both P < 0.05) than cKP strains. However, neither biofilm formation nor bactericidal effect of serum was found with significant difference in between ESBL producing cKP and ESBL producing hvKP strains (both P > 0.05). Although the hvKP possess more virulence gene, but they didn’t show any significant difference between biofilm formation and bactericidal effect of serum compared with ESBL producing cKP strains.

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical <italic>Klebsiella pneumoniae</italic> and Hypervirulent <italic>Klebsiella pneumoniae</italic> from Respiratory Tract Infected Patients

Department of Pathogenobiology (Microbiology), The Key Laboratory of Zoonosis

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical  and Hypervirulent  from Respiratory Tract Infected Patients

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with capsule-associated mucopolysaccharide web is known as hypervirulent K. pneumoniae (hvKP) as it enhances the serum resistant and biofilm production. Aim is to determine and correlate various virulence genes, ESBL, serum bactericidal effect and biofilm formation of clinical isolated cKP and hvKP from respiratory tract infected patients. A total of 96 K. pneumoniae strains were isolated from sputum of respiratory tract infected patients. The isolates were performed string test, AST, ESBL virulence gene, serum bactericidal and biofilm assays. Out of 96 isolates, 39 isolates (40.6%) were identified with hypervirulent phenotypes. The number of cKP exhibiting resistance to the tested antimicrobials and ESBLs were significantly higher than that of the hvKP strains. The virulence genes of K. pneumoniae such as K1, K2, rmpA, uge, kfu and aerobactin were strongly associated with hvKP than cKP. However, no significant difference was found in FIM-1 and MrKD3 genes. ESBL producing cKP and hvKP were significantly associated with strong biofilm formation (both P  0.05). Although the hvKP possess more virulence gene, but they didn’t show any significant difference between biofilm formation and bactericidal effect of serum compared with ESBL producing cKP strains.

Klebsiella pneumoniae strains that are commonly recognized by clinicians and microbiologists are termed as classical K. pneumoniae (cKP). A strain with...

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical Klebsiella pneumoniae and Hypervirulent Klebsiella pneumoniae from Respiratory Tract Infected Patients

Publicado en línea: 04 dic 2017

Páginas: 501 - 508

Recibido: 02 mar 2016

Aceptado: 04 abr 2017

DOI: https://doi.org/10.5604/pjm-2017-501

Palabras clave
biofilm, ESBL, classical hypervirulent, serum resistance, virulence genes

© 2017 RAMBHA K. SHAH et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Determination and Correlation of Various Virulence Genes, ESBL, Serum Bactericidal Effect and Biofilm Formation of Clinical Isolated Classical Klebsiella pneumoniae and Hypervirulent Klebsiella pneumoniae from Respiratory Tract Infected Patients

Publicado en línea: 04 dic 2017

Páginas: 501 - 508

Recibido: 02 mar 2016

Aceptado: 04 abr 2017

DOI: https://doi.org/10.5604/pjm-2017-501

Palabras clavebiofilm, ESBL, classical hypervirulent, serum resistance, virulence genes

© 2017 RAMBHA K. SHAH et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
biofilm, ESBL, classical hypervirulent, serum resistance, virulence genes