<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Streptococcus anginosus</italic>, <italic>Streptococcus intermedius</italic> and <italic>Streptococcus constellatus</italic> form a group of related streptococcal species, namely the Streptococcus Anginosus Group (SAG). The group, previously called “milleri” had been rarely described until 1980/1990 as source of infections. Nowadays SAG bacteria are often described as pathogens causing predominantly purulent infections. The number of infections is highly underestimated, as SAG strains are often classified in the microbiology laboratory as less virulent “viridans streptococci”. Epidemiological situation regarding SAG infections in Poland has been unrecognized, therefore we performed a retrospective analysis of strains isolated between 1996 and 2012. Strains suspected of belonging to SAG were re-identified using an automated biochemical approach (Vitek2) and MALDI-TOF MS. We performed first analysis of antibiotic resistance among SAG strains isolated in Poland using automated methods (Vitek2), disk diffusion tests and E-Tests. We also performed PCR detection of resistance determinants in antibiotic resistant strains. Clonal structure of analyzed strains was evaluated with PFGE and MLVF methods. All three species are difficult to distinguish using automated diagnostic methods and the same is true for automated MIC evaluation. Our analysis revealed SAG strains are rarely isolated in Poland, predominantly from purulent infections. All isolates are very diverse on the genomic level as estimated by PFGE and MLVF analyses. All analyzed strains are sensitive to penicillin, a substantial group of strains is resistant to macrolides and the majority of strains are resistant to tetracycline.</p>
</abstract>

Streptococcus anginosus, Streptococcus intermedius and Streptococcus constellatus form a group of related streptococcal species, namely the Streptococcus Anginosus Group (SAG). The group, previously called “milleri” had been rarely described until 1980/1990 as source of infections. Nowadays SAG bacteria are often described as pathogens causing predominantly purulent infections. The number of infections is highly underestimated, as SAG strains are often classified in the microbiology laboratory as less virulent “viridans streptococci”. Epidemiological situation regarding SAG infections in Poland has been unrecognized, therefore we performed a retrospective analysis of strains isolated between 1996 and 2012. Strains suspected of belonging to SAG were re-identified using an automated biochemical approach (Vitek2) and MALDI-TOF MS. We performed first analysis of antibiotic resistance among SAG strains isolated in Poland using automated methods (Vitek2), disk diffusion tests and E-Tests. We also performed PCR detection of resistance determinants in antibiotic resistant strains. Clonal structure of analyzed strains was evaluated with PFGE and MLVF methods. All three species are difficult to distinguish using automated diagnostic methods and the same is true for automated MIC evaluation. Our analysis revealed SAG strains are rarely isolated in Poland, predominantly from purulent infections. All isolates are very diverse on the genomic level as estimated by PFGE and MLVF analyses. All analyzed strains are sensitive to penicillin, a substantial group of strains is resistant to macrolides and the majority of strains are resistant to tetracycline.

<italic>Streptococcus anginosus</italic> (<italic>milleri</italic>) Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 () Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

Streptococcus anginosus, Streptococcus intermedius and Streptococcus constellatus form a group of related streptococcal species, namely the Streptococcus...

Streptococcus anginosus (milleri) Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

Publicado en línea: 15 mar 2016

Páginas: 33 - 41

Recibido: 01 dic 2015

Aceptado: 21 dic 2015

DOI: https://doi.org/10.5604/17331331.1197323

Palabras clave
group, antimicrobial resistance, MLVA, MLVF, PFGE

© 2016 KATARZYNA OBSZAŃSKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Streptococcus anginosus (milleri) Group Strains Isolated in Poland (1996–2012) and their Antibiotic Resistance Patterns

Publicado en línea: 15 mar 2016

Páginas: 33 - 41

Recibido: 01 dic 2015

Aceptado: 21 dic 2015

DOI: https://doi.org/10.5604/17331331.1197323

Palabras clavegroup, antimicrobial resistance, MLVA, MLVF, PFGE

© 2016 KATARZYNA OBSZAŃSKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
group, antimicrobial resistance, MLVA, MLVF, PFGE