<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.1003.483_s_001_w2aab3b7b1b1b6b1aab1c16b1Aa"><h3>Background</h3><p>The emergence of extended-spectrum β-lactamase (ESBL)-producing <italic>Escherichia coli</italic> and <italic>Klebsiella pneumoniae</italic> constitutes a serious problem because of the transfer of resistance genes from one organism to another.</p></sec><sec id="j_1905-7415.1003.483_s_002_w2aab3b7b1b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To screen the antibiotic susceptibility of <italic>E. coli</italic> and <italic>K. pneumoniae</italic> from inpatients at King Abdul- Aziz Hospital, Al-Taif, Saudi Arabia and to detect common ESBL genes, and random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) genotyping of the Enterobacteriaceae.</p></sec><sec id="j_1905-7415.1003.483_s_003_w2aab3b7b1b1b6b1aab1c16b3Aa"><h3>Methods</h3><p>We analyzed 43 bacterial strains isolated from clinical samples of sporadic cases for their reactivity to different antibiotics. Strains that showed resistance by monoplex PCR were screened for ESBL TEM- and CTXM-1-, -2-, and -9-encoding genes. Random amplifications were used for typing the resistant strains.</p></sec><sec id="j_1905-7415.1003.483_s_004_w2aab3b7b1b1b6b1aab1c16b4Aa"><h3>Results</h3><p>The majority of the ESBL containing strains were sensitive to meropenem, amikacin, gentamicin, and tigecycline. PCR detection using ESBL gene-specific primers showed that 17 of 43 strains harbored genes for ESBL TEM, CTX-M-1, or CTX-M-2. RAPD typing revealed marked variation among the ESBL-producing <italic>E.coli</italic> in relation to each other.</p></sec><sec id="j_1905-7415.1003.483_s_005_w2aab3b7b1b1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>Considerably high incidence of ESBL-producing bacteria was present patients from the local area of Al-Taif and nearby cities in Saudi Arabia with TEM and CTX-M subtypes being the most commonly detected variants. There was evidence of a polymorphic genetic pattern among ESBL-producing bacteria.</p></sec></abstract>

BackgroundThe emergence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae constitutes a serious problem because of the transfer of resistance genes from one organism to another.ObjectivesTo screen the antibiotic susceptibility of E. coli and K. pneumoniae from inpatients at King Abdul- Aziz Hospital, Al-Taif, Saudi Arabia and to detect common ESBL genes, and random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) genotyping of the Enterobacteriaceae.MethodsWe analyzed 43 bacterial strains isolated from clinical samples of sporadic cases for their reactivity to different antibiotics. Strains that showed resistance by monoplex PCR were screened for ESBL TEM- and CTXM-1-, -2-, and -9-encoding genes. Random amplifications were used for typing the resistant strains.ResultsThe majority of the ESBL containing strains were sensitive to meropenem, amikacin, gentamicin, and tigecycline. PCR detection using ESBL gene-specific primers showed that 17 of 43 strains harbored genes for ESBL TEM, CTX-M-1, or CTX-M-2. RAPD typing revealed marked variation among the ESBL-producing E.coli in relation to each other.ConclusionsConsiderably high incidence of ESBL-producing bacteria was present patients from the local area of Al-Taif and nearby cities in Saudi Arabia with TEM and CTX-M subtypes being the most commonly detected variants. There was evidence of a polymorphic genetic pattern among ESBL-producing bacteria.

Antibiotic resistance profile and random amplification typing of β-lactamase-producing Enterobacteriaceae from the local area of Al-Taif and nearby cities in Saudi Arabia

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

BackgroundThe emergence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae constitutes a serious problem because of...

Antibiotic resistance profile and random amplification typing of β-lactamase-producing Enterobacteriaceae from the local area of Al-Taif and nearby cities in Saudi Arabia

Article Category: Original article

Publicado en línea: 31 ene 2017

Páginas: 219 - 228

DOI: https://doi.org/10.5372/1905-7415.1003.483

Palabras clave
Antibiotic resistance, RAPD typing, Saudi Arabia

© 2016 Ali K. Alzahrani, Mostafa M. Farag, Said H. Abbadi, Mohamed M. Hassan, Ahmed Gaber, Ahmed S. Abdel-Moneim

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antibiotic resistance profile and random amplification typing of β-lactamase-producing Enterobacteriaceae from the local area of Al-Taif and nearby cities in Saudi Arabia

Article Category: Original article

Publicado en línea: 31 ene 2017

Páginas: 219 - 228

DOI: https://doi.org/10.5372/1905-7415.1003.483

Palabras claveAntibiotic resistance, RAPD typing, Saudi Arabia

© 2016 Ali K. Alzahrani, Mostafa M. Farag, Said H. Abbadi, Mohamed M. Hassan, Ahmed Gaber, Ahmed S. Abdel-Moneim

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Antibiotic resistance, RAPD typing, Saudi Arabia