<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0901.369_s_101_w2aab3b7b9b1b6b1aab1c16b1Aa"><h3>Background</h3><p><italic>KRAS</italic>, coding for a small G-protein downstream of epidermal growth factor receptor (EGFR) plays an important role in the EGFR signaling network. Mutation in <italic>KRAS</italic> is associated with resistance to anti-EGFR in patients with advanced colorectal cancer (CRC). According to the American Society of Clinical Oncology (ASCO) guidelines, screening for mutations in <italic>KRAS</italic> codons 12 and 13 in tumor samples is mandatory for all CRC patients who are candidates for anti-EGFR targeted therapy. However, some patients with undetectable mutations in codons 12/13 do not benefit from anti-EGFR treatment, and this might be because of mutations in codon 61, which is not currently recommended for screening.</p></sec><sec id="j_1905-7415.0901.369_s_102_w2aab3b7b9b1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To develop an in-house pyrosequencing method to screen for <italic>KRAS</italic> codon 61 mutations, and examine the prevalence of mutations in Thai patients with advanced CRC with no detectable mutation in codons 12/13.</p></sec><sec id="j_1905-7415.0901.369_s_103_w2aab3b7b9b1b6b1aab1c16b3Aa"><h3>Materials and Methods</h3><p>DNA extracted from FFPE specimens was screened for <italic>KRAS</italic> codon 61 mutations using pyrosequencing. Our method was suitable for routine clinical samples (formalin-fixed, paraffin-embedded tissue), and was able to detect 5 common mutations in codon 61 of the <italic>KRAS</italic> gene, including c.182AT (p.Q61L), c.182AG (p.Q61R), c.182AC (p.Q61P), c.183AC (p.Q61H), and c.183AT (p.Q61H).</p></sec><sec id="j_1905-7415.0901.369_s_104_w2aab3b7b9b1b6b1aab1c16b4Aa"><h3>Results</h3><p>Of the 74 samples with undetectable codon 12/13 mutation examined, two (2.7%) were found to harbor mutation in codon 61.</p></sec><sec id="j_1905-7415.0901.369_s_105_w2aab3b7b9b1b6b1aab1c16b5Aa"><h3>Conclusion</h3><p>Despite the low prevalence of <italic>KRAS</italic> codon 61 mutation in our population with advanced CRC, adding the mutation test into the routine molecular service deserves consideration because the cost of treatment is very expensive.</p></sec></abstract>

BackgroundKRAS, coding for a small G-protein downstream of epidermal growth factor receptor (EGFR) plays an important role in the EGFR signaling network. Mutation in KRAS is associated with resistance to anti-EGFR in patients with advanced colorectal cancer (CRC). According to the American Society of Clinical Oncology (ASCO) guidelines, screening for mutations in KRAS codons 12 and 13 in tumor samples is mandatory for all CRC patients who are candidates for anti-EGFR targeted therapy. However, some patients with undetectable mutations in codons 12/13 do not benefit from anti-EGFR treatment, and this might be because of mutations in codon 61, which is not currently recommended for screening.ObjectivesTo develop an in-house pyrosequencing method to screen for KRAS codon 61 mutations, and examine the prevalence of mutations in Thai patients with advanced CRC with no detectable mutation in codons 12/13.Materials and MethodsDNA extracted from FFPE specimens was screened for KRAS codon 61 mutations using pyrosequencing. Our method was suitable for routine clinical samples (formalin-fixed, paraffin-embedded tissue), and was able to detect 5 common mutations in codon 61 of the KRAS gene, including c.182AT (p.Q61L), c.182AG (p.Q61R), c.182AC (p.Q61P), c.183AC (p.Q61H), and c.183AT (p.Q61H).ResultsOf the 74 samples with undetectable codon 12/13 mutation examined, two (2.7%) were found to harbor mutation in codon 61.ConclusionDespite the low prevalence of KRAS codon 61 mutation in our population with advanced CRC, adding the mutation test into the routine molecular service deserves consideration because the cost of treatment is very expensive.

Pyrosequencing analysis of <italic>KRAS</italic> codon 61 mutations in Thai patients with advanced colorectal cancer

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Pyrosequencing analysis of  codon 61 mutations in Thai patients with advanced colorectal cancer

BackgroundKRAS, coding for a small G-protein downstream of epidermal growth factor receptor (EGFR) plays an important role in the EGFR signaling network....

Pyrosequencing analysis of KRAS codon 61 mutations in Thai patients with advanced colorectal cancer

Article Category: Brief communication (Original)

Publicado en línea: 31 ene 2017

Páginas: 61 - 67

DOI: https://doi.org/10.5372/1905-7415.0901.369

Palabras clave
Colorectal cancer, KRAS, mutation, pyrosequencing, targeted therapy

© 2017 Chinachote Teerapakpinyo, Phanni Wanthong, Mathawee Aumchaaumchaya, Piyamai Chankate, Warisa Kaikeaw, Warunya Tosakorn, Shanop Shaungshoti

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Pyrosequencing analysis of KRAS codon 61 mutations in Thai patients with advanced colorectal cancer

Article Category: Brief communication (Original)

Publicado en línea: 31 ene 2017

Páginas: 61 - 67

DOI: https://doi.org/10.5372/1905-7415.0901.369

Palabras claveColorectal cancer, KRAS, mutation, pyrosequencing, targeted therapy

© 2017 Chinachote Teerapakpinyo, Phanni Wanthong, Mathawee Aumchaaumchaya, Piyamai Chankate, Warisa Kaikeaw, Warunya Tosakorn, Shanop Shaungshoti

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Colorectal cancer, KRAS, mutation, pyrosequencing, targeted therapy