<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Pyridostigmine is a reversible acetylcholinesterase inhibitor that is used in military medicine as a prophylactic agent against intoxication by nerve agents and in the treatment of myasthenia.Objective: We developed and validated a high-performance liquid chromatography (HPLC) method for the quantification of pyridostigmine in plasma samples.Methods: Pyridostigmine was isolated from plasma using one-step protein precipitation by chloroform. The separation was performed on an analytical C18 column (250 × 4.6 mm; 4 μm particle size). The detector wavelength was set at 270 nm. The mobile phase was a mixture of acetonitrile and aqueous solution (15:85 v/v) of sodium-1-hepatene sulfonate and triethylamine, adjusted to pH 3.0 at a flow rate of 1.5 ml min-1. Hydrochlorothiazide was used as internal standard.Results: The recovery of drug from plasma samples was above 90%. Using the current method, pyridostigmine (rt = 7.3) and hydrochlorothiazide (rt = 6.4) peaks were well resolved. The calibration curve was linear over the concentration range of 15-60 ng ml-1. The inter- and intraday assay coefficients of variation were found to be less than 8%.Conclusion: The currently described procedure could be used as a simple, rapid and sensitive tool in bioavailability and bioequivalency investigations for the quantification of pyridostigmine in human plasma samples.</abstract>

Background: Pyridostigmine is a reversible acetylcholinesterase inhibitor that is used in military medicine as a prophylactic agent against intoxication by nerve agents and in the treatment of myasthenia.Objective: We developed and validated a high-performance liquid chromatography (HPLC) method for the quantification of pyridostigmine in plasma samples.Methods: Pyridostigmine was isolated from plasma using one-step protein precipitation by chloroform. The separation was performed on an analytical C18 column (250 × 4.6 mm; 4 μm particle size). The detector wavelength was set at 270 nm. The mobile phase was a mixture of acetonitrile and aqueous solution (15:85 v/v) of sodium-1-hepatene sulfonate and triethylamine, adjusted to pH 3.0 at a flow rate of 1.5 ml min-1. Hydrochlorothiazide was used as internal standard.Results: The recovery of drug from plasma samples was above 90%. Using the current method, pyridostigmine (rt = 7.3) and hydrochlorothiazide (rt = 6.4) peaks were well resolved. The calibration curve was linear over the concentration range of 15-60 ng ml-1. The inter- and intraday assay coefficients of variation were found to be less than 8%.Conclusion: The currently described procedure could be used as a simple, rapid and sensitive tool in bioavailability and bioequivalency investigations for the quantification of pyridostigmine in human plasma samples.

Brief communication (Original). Validation of a high-performance liquid chromatography method for the determination of pyridostigmine in plasma

Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran

Biotechnology Research Center and School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Tehran,

Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran,

Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran

Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran

Noor Research and Educational Institute,Baqiyatallah University of Medical Sciences, Tehran,

Department of Internal Medicine, Baqiyatallah University of Medical Sciences, Tehran,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Pyridostigmine is a reversible acetylcholinesterase inhibitor that is used in military medicine as a prophylactic agent against intoxication by...

Brief communication (Original). Validation of a high-performance liquid chromatography method for the determination of pyridostigmine in plasma

Publicado en línea: 04 feb 2017

Páginas: 275 - 279

DOI: https://doi.org/10.5372/1905-7415.0702.176

Palabras clave
Drug, high-performance liquid chromatography, Mestinon, method validation

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Brief communication (Original). Validation of a high-performance liquid chromatography method for the determination of pyridostigmine in plasma

Publicado en línea: 04 feb 2017

Páginas: 275 - 279

DOI: https://doi.org/10.5372/1905-7415.0702.176

Palabras claveDrug, high-performance liquid chromatography, Mestinon, method validation

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
Drug, high-performance liquid chromatography, Mestinon, method validation