<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Mesenchymal stem cells (MSCs) known to be sensitive to mechanical stimulus. This type of stimulus plays a role in cellular differentiation, so that it might affect MSCs differentiation toward cardiomyocytes.Objectives: Investigate the effect of mechanical stimulus on MSCs differentiation toward cardiomyocytes.Methods: The adipose tissue-derived MSCs were induced to differentiate with 5-azacytidine, and stimulated by one Hz mechanical stretching up to 8%. After 10 days, the cell’s cardiac markers and cardiogenesis-related genes were detected by immumohistochemistrical staining and reverse transcriptase-polymerase chain reaction, and the cell’s ATPase activity was detected.Results: The cyclic mechanical stretching enhanced the expression of cardiogenesis-related genes and cardiac markers, and stimulated the activity of Na+-K+-ATPase and Ca2+-ATPase in the MSCs treated with 5-azacytidine. Without 5-azacytidine pre-treatment, cyclic mechanical stretch alone has little effect.Conclusion: Mechanical stretch combined with 5-azacytidine treatment could accelerate MSCs differentiation toward cardiomyocytes.</abstract>

Background: Mesenchymal stem cells (MSCs) known to be sensitive to mechanical stimulus. This type of stimulus plays a role in cellular differentiation, so that it might affect MSCs differentiation toward cardiomyocytes.Objectives: Investigate the effect of mechanical stimulus on MSCs differentiation toward cardiomyocytes.Methods: The adipose tissue-derived MSCs were induced to differentiate with 5-azacytidine, and stimulated by one Hz mechanical stretching up to 8%. After 10 days, the cell’s cardiac markers and cardiogenesis-related genes were detected by immumohistochemistrical staining and reverse transcriptase-polymerase chain reaction, and the cell’s ATPase activity was detected.Results: The cyclic mechanical stretching enhanced the expression of cardiogenesis-related genes and cardiac markers, and stimulated the activity of Na+-K+-ATPase and Ca2+-ATPase in the MSCs treated with 5-azacytidine. Without 5-azacytidine pre-treatment, cyclic mechanical stretch alone has little effect.Conclusion: Mechanical stretch combined with 5-azacytidine treatment could accelerate MSCs differentiation toward cardiomyocytes.

Effects of mechanical stimulus on mesenchymal stem cells differentiation toward cardiomyocytes

Tianjin Institute of Medical Equipment, Tianjin 300161,

School of Mechanical Engineering, Tianjin University of Technology, Tianjin 300191,

Biology Department, Dezhou University, Dezhou 253021,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Mesenchymal stem cells (MSCs) known to be sensitive to mechanical stimulus. This type of stimulus plays a role in cellular differentiation, so that...

Effects of mechanical stimulus on mesenchymal stem cells differentiation toward cardiomyocytes

Article Category: Brief communication (Original)

Publicado en línea: 04 feb 2017

Páginas: 655 - 661

DOI: https://doi.org/10.5372/1905-7415.0505.096

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.