Significance of Fusobacterium nucleatum Combined with SFRP2 and SDC2 Gene Methylation Detection in Early Screening of Colorectal Cancer

Ahlquist DA, Zou H, Domanico M, Mahoney DW, Yab TC, Taylor WR, Butz ML, Thibodeau SN, Rabeneck L, Paszat LF, et al. Next-generation stool DNA test accurately detects colorectal cancer and large adenomas. Gastroenterology. 2012 Feb;142(2):248–256. https://doi.org/10.1053/j.gastro.2011.10.031 Ahlquist DA Zou H Domanico M Mahoney DW Yab TC Taylor WR Butz ML Thibodeau SN Rabeneck L Paszat LF Next-generation stool DNA test accurately detects colorectal cancer and large adenomas . Gastroenterology . 2012 Feb ; 142 ( 2 ): 248 – 256 . https://doi.org/10.1053/j.gastro.2011.10.031 Search in Google Scholar

Aitchison A, Pearson JF, Purcell RV, Frizelle FA, Keenan JI. Detection of Fusobacterium nucleatum DNA in primary care patient stool samples does not predict progression of colorectal neoplasia. PLoS One. 2022 Jun;17(6):e0269541. https://doi.org/10.1371/journal.pone.0269541 Aitchison A Pearson JF Purcell RV Frizelle FA Keenan JI. Detection of Fusobacterium nucleatum DNA in primary care patient stool samples does not predict progression of colorectal neoplasia . PLoS One . 2022 Jun ; 17 ( 6 ): e0269541 . https://doi.org/10.1371/jour-nal.pone.0269541 Search in Google Scholar

Borozan I, Zaidi SH, Harrison TA, Phipps AI, Zheng J, Lee S, Trinh QM, Steinfelder RS, Adams J, Banbury BL, et al. Molecular and pathology features of colorectal tumors and patient outcomes are associated with Fusobacterium nucleatum and its subspecies animalis. Cancer Epidemiol Biomarkers Prev. 2022 Jan;31(1):210–220. https://doi.org/10.1158/1055-9965.epi-21-0463 Borozan I Zaidi SH Harrison TA Phipps AI Zheng J Lee S Trinh QM Steinfelder RS Adams J Banbury BL Molecular and pathology features of colorectal tumors and patient outcomes are associated with Fusobacterium nucleatum and its subspecies animalis . Cancer Epidemiol Biomarkers Prev . 2022 Jan ; 31 ( 1 ): 210 – 220 . https://doi.org/10.1158/1055-9965.epi-21-0463 Search in Google Scholar

Bosch LJ, Carvalho B, Fijneman RJ, Jimenez CR, Pinedo HM, van Engeland M, Meijer GA. Molecular tests for colorectal cancer screening. Clin Colorectal Cancer. 2011 Mar;10(1):8–23. https://doi.org/10.3816/ccc.2011.n.002 Bosch LJ Carvalho B Fijneman RJ Jimenez CR Pinedo HM van Engeland M Meijer GA. Molecular tests for colorectal cancer screening . Clin Colorectal Cancer . 2011 Mar ; 10 ( 1 ): 8 – 23 . https://doi.org/10.3816/ccc.2011.n.002 Search in Google Scholar

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 Nov;68(6):394–424. https://doi.org/10.3322/caac.21492 Bray F Ferlay J Soerjomataram I Siegel RL Torre LA Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries . CA Cancer J Clin . 2018 Nov ; 68 ( 6 ): 394 – 424 . https://doi.org/10.3322/caac.21492 Search in Google Scholar

Chandrashekar DS, Bashel B, Balasubramanya SAH, Creighton CJ, Ponce-Rodriguez I, Chakravarthi BVSK, Varambally S. UAL-CAN: A portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia. 2017 Aug;19(8):649–658. https://doi.org/10.1016/j.neo.2017.05.002 Chandrashekar DS Bashel B Balasubramanya SAH Creighton CJ Ponce-Rodriguez I Chakravarthi BVSK Varambally S. UAL-CAN: A portal for facilitating tumor subgroup gene expression and survival analyses . Neoplasia . 2017 Aug ; 19 ( 8 ): 649 – 658 . https://doi.org/10.1016/j.neo.2017.05.002 Search in Google Scholar

Chandrashekar DS, Karthikeyan SK, Korla PK, Patel H, Shovon AR, Athar M, Netto GJ, Qin ZS, Kumar S, Manne U, et al. UALCAN: An update to the integrated cancer data analysis platform. Neoplasia. 2022 Mar;25:18–27. https://doi.org/10.1016/j.neo.2022.01.001 Chandrashekar DS Karthikeyan SK Korla PK Patel H Shovon AR Athar M Netto GJ Qin ZS Kumar S Manne U UALCAN: An update to the integrated cancer data analysis platform . Neoplasia . 2022 Mar ; 25 : 18 – 27 . https://doi.org/10.1016/j.neo.2022.01.001 Search in Google Scholar

Datorre JG, Dos Reis MB, de Carvalho AC, Porto J, Rodrigues GH, Lima AB, Reis MT, Hirai W, Hashimoto CL, Guimarães DP, et al. Enhancing colorectal cancer screening with droplet digital PCR analysis of Fusobacterium nucleatum in fecal immunochemical test samples. Cancer Prev Res (Phila). 2024 Oct;17(10):471–479. https://doi.org/10.1158/1940-6207.capr-23-0331 Datorre JG Dos Reis MB de Carvalho AC Porto J Rodrigues GH Lima AB Reis MT Hirai W Hashimoto CL Guimarães DP Enhancing colorectal cancer screening with droplet digital PCR analysis of Fusobacterium nucleatum in fecal immunochemical test samples . Cancer Prev Res (Phila) . 2024 Oct ; 17 ( 10 ): 471 – 479 . https://doi.org/10.1158/1940-6207.capr-23-0331 Search in Google Scholar

Dohlman AB, Arguijo Mendoza D, Ding S, Gao M, Dressman H, Iliev ID, Lipkin SM, Shen X. The cancer microbiome atlas: a pan-cancer comparative analysis to distinguish tissue-resident microbiota from contaminants. Cell Host Microbe. 2021 Feb;29(2):281–298.e5. https://doi.org/10.1016/j.chom.2020.12.001 Dohlman AB Arguijo Mendoza D Ding S Gao M Dressman H Iliev ID Lipkin SM Shen X. The cancer microbiome atlas: a pan-cancer comparative analysis to distinguish tissue-resident microbiota from contaminants . Cell Host Microbe . 2021 Feb ; 29 ( 2 ): 281 – 298 .e5. https://doi.org/10.1016/j.chom.2020.12.001 Search in Google Scholar

Fardini Y, Wang X, Témoin S, Nithianantham S, Lee D, Shoham M, Han YW. Fusobacterium nucleatum adhesin FadA binds vascular endothelial cadherin and alters endothelial integrity. Mol Microbiol. 2011 Dec;82(6):1468–1480. https://doi.org/10.1111/j.1365-2958.2011.07905.x Fardini Y Wang X Témoin S Nithianantham S Lee D Shoham M Han YW. Fusobacterium nucleatum adhesin FadA binds vascular endothelial cadherin and alters endothelial integrity . Mol Microbiol . 2011 Dec ; 82 ( 6 ): 1468 – 1480 . https://doi.org/10.1111/j.1365-2958.2011.07905.x Search in Google Scholar

Gao Y, Bi D, Xie R, Li M, Guo J, Liu H, Guo X, Fang J, Ding T, Zhu H, et al. Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer. Signal Transduct Target Ther. 2021 Nov 19;6(1):398. https://doi.org/10.1038/s41392-021-00795-x Gao Y Bi D Xie R Li M Guo J Liu H Guo X Fang J Ding T Zhu H Fusobacterium nucleatum enhances the efficacy of PD-L1 blockade in colorectal cancer . Signal Transduct Target Ther . 2021 Nov 19 ; 6 ( 1 ): 398 . https://doi.org/10.1038/s41392-021-00795-x Search in Google Scholar

Hua R, Yu J, Yan X, Ni Q, Zhi X, Li X, Jiang B, Zhu J. Syndecan-2 in colorectal cancer plays oncogenic role via epithelial-mesenchymal transition and MAPK pathway. Biomed Pharmacother. 2020 Jan;121:109630. https://doi.org/10.1016/j.biopha.2019.109630 Hua R Yu J Yan X Ni Q Zhi X Li X Jiang B Zhu J. Syndecan-2 in colorectal cancer plays oncogenic role via epithelial-mesenchymal transition and MAPK pathway . Biomed Pharmacother . 2020 Jan ; 121 : 109630 . https://doi.org/10.1016/j.biopha.2019.109630 Search in Google Scholar

Huang S, Yang Z, Zou D, Dong D, Liu A, Liu W, Huang L. Rapid detection of nusG and fadA in Fusobacterium nucleatum by loop-mediated isothermal amplification. J Med Microbiol. 2016 Aug;65(8):760–769. https://doi.org/10.1099/jmm.0.000300 Huang S Yang Z Zou D Dong D Liu A Liu W Huang L. Rapid detection of nusG and fadA in Fusobacterium nucleatum by loop-mediated isothermal amplification . J Med Microbiol . 2016 Aug ; 65 ( 8 ): 760 – 769 . https://doi.org/10.1099/jmm.0.000300 Search in Google Scholar

Ikegami A, Chung P, Han YW. Complementation of the fadA mutation in Fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization. Infect Immun. 2009 Jul;77(7):3075–3079. https://doi.org/10.1128/iai.00209-09 Ikegami A Chung P Han YW. Complementation of the fadA mutation in Fusobacterium nucleatum demonstrates that the surface-exposed adhesin promotes cellular invasion and placental colonization . Infect Immun . 2009 Jul ; 77 ( 7 ): 3075 – 3079 . https://doi.org/10.1128/iai.00209-09 Search in Google Scholar

Irrazábal T, Belcheva A, Girardin SE, Martin A, Philpott DJ. The multifaceted role of the intestinal microbiota in colon cancer. Mol Cell. 2014 Apr;54(2):309–320. https://doi.org/10.1016/j.mol-cel.2014.03.039 Irrazábal T Belcheva A Girardin SE Martin A Philpott DJ. The multifaceted role of the intestinal microbiota in colon cancer . Mol Cell . 2014 Apr ; 54 ( 2 ): 309 – 320 . https://doi.org/10.1016/j.mol-cel.2014.03.039 Search in Google Scholar

Issa IA, Noureddine M. Colorectal cancer screening: An updated review of the available options. World J Gastroenterol. 2017 Jul;23(28):5086–5096. https://doi.org/10.3748/wjg.v23.i28.5086 Issa IA Noureddine M. Colorectal cancer screening: An updated review of the available options . World J Gastroenterol . 2017 Jul ; 23 ( 28 ): 5086 – 5096 . https://doi.org/10.3748/wjg.v23.i28.5086 Search in Google Scholar

Leporrier J, Maurel J, Chiche L, Bara S, Segol P, Launoy G. A population-based study of the incidence, management and prognosis of hepatic metastases from colorectal cancer. Br J Surg. 2006 Apr;93(4):465–474. https://doi.org/10.1002/bjs.5278 Leporrier J Maurel J Chiche L Bara S Segol P Launoy G. A population-based study of theincidence management and prognosis of hepatic metastases from colorectal cancer . Br J Surg . 2006 Apr ; 93 ( 4 ): 465 – 474 . https://doi.org/10.1002/bjs.5278 Search in Google Scholar

Li B, Liu S, Gao Y, Zheng L, Lu Y. Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening. J Cancer Res Clin Oncol. 2023 Sep;149(12):10241–10253. https://doi.org/10.1007/s00432-023-04943-4 Li B Liu S Gao Y Zheng L Lu Y. Combined detection of SDC2/ADHFE1/PPP2R5C methylation in stool DNA for colorectal cancer screening . J Cancer Res Clin Oncol . 2023 Sep ; 149 ( 12 ): 10241 – 10253 . https://doi.org/10.1007/s00432-023-04943-4 Search in Google Scholar

Li B, Severson E, Pignon JC, Zhao H, Li T, Novak J, Jiang P, Shen H, Aster JC, Rodig S, Signoretti S, Liu JS, Liu XS. Comprehensive analyses of tumor immunity: implications for cancer immunotherapy. Genome Biol. 2016 Aug 22;17(1):174. https://doi.org/10.1186/s13059-016-1028-7 Li B Severson E Pignon JC Zhao H Li T Novak J Jiang P Shen H Aster JC Rodig S Signoretti S Liu JS Liu XS. Comprehensive analyses of tumor immunity: implications for cancer immunotherapy . Genome Biol . 2016 Aug 22 ; 17 ( 1 ): 174 . https://doi.org/10.1186/s13059-016-1028-7 Search in Google Scholar

Li T, Fan J, Wang B, Traugh N, Chen Q, Liu JS, Li B, Liu XS. TIMER: A Web Server for Comprehensive Analysis of Tumor-Infiltrating Immune Cells. Cancer Res. 2017 Nov;77(21):e108–e110. https://doi.org/10.1158/0008-5472.CAN-17-0307 Li T Fan J Wang B Traugh N Chen Q Liu JS Li B Liu XS. TIMER: A Web Server for Comprehensive Analysis of Tumor-Infiltrating Immune Cells . Cancer Res . 2017 Nov ; 77 ( 21 ): e108 – e110 . https://doi.org/10.1158/0008-5472.CAN-17-0307 Search in Google Scholar

Mima K, Nishihara R, Qian ZR, Cao Y, Sukawa Y, Nowak JA, Yang J, Dou R, Masugi Y, Song M, et al. Fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis. Gut. 2016 Dec;65(12):1973–1980. https://doi.org/10.1136/gut-jnl-2015-310101 Mima K Nishihara R Qian ZR Cao Y Sukawa Y Nowak JA Yang J Dou R Masugi Y Song M Fusobacterium nu-cleatum in colorectal carcinoma tissue and patient prognosis . Gut . 2016 Dec ; 65 ( 12 ): 1973 – 1980 . https://doi.org/10.1136/gut-jnl-2015-310101 Search in Google Scholar

Nguyen LH, Goel A, Chung DC. Pathways of colorectal carcinogenesis. Gastroenterology. 2020 Jan;158(2):291–302. https://doi.org/10.1053/j.gastro.2019.08.059 Nguyen LH Goel A Chung DC. Pathways of colorectal carcinogenesis . Gastroenterology . 2020 Jan ; 158 ( 2 ): 291 – 302 . https://doi.org/10.1053/j.gastro.2019.08.059 Search in Google Scholar

Niu F, Wen J, Fu X, Li C, Zhao R, Wu S, Yu H, Liu X, Zhao X, Liu S, et al. Stool DNA test of methylated Syndecan-2 for the early detection of colorectal neoplasia. Cancer Epidemiol Biomarkers Prev. 2017 Sep;26(9):1411–1419. https://doi.org/10.1158/1055-9965.epi-17-0153 Niu F Wen J Fu X Li C Zhao R Wu S Yu H Liu X Zhao X Liu S Stool DNA test of methylated Syndecan-2 for the early detection of colorectal neoplasia . Cancer Epidemiol Biomarkers Prev . 2017 Sep ; 26 ( 9 ): 1411 – 1419 . https://doi.org/10.1158/1055-9965.epi-17-0153 Search in Google Scholar

Oberwalder M, Zitt M, Wöntner C, Fiegl H, Goebel G, Zitt M, Köhle O, Mühlmann G, Ofner D, Margreiter R, et al. SFRP2 methylation in fecal DNA – a marker for colorectal polyps. Int J Colorectal Dis. 2008 Jan;23(1):15–19. https://doi.org/10.1007/s00384-007-0355-2 Oberwalder M Zitt M Wöntner C Fiegl H Goebel G Zitt M Köhle O Mühlmann G Ofner D Margreiter R SFRP2 methylation in fecal DNA – a marker for colorectal polyps . Int J Colorectal Dis . 2008 Jan ; 23 ( 1 ): 15 – 19 . https://doi.org/10.1007/s00384-007-0355-2 Search in Google Scholar

Osborn NK, Ahlquist DA. Stool screening for colorectal cancer: Molecular approaches. Gastroenterology. 2005 Jan;128(1):192–206. https://doi.org/10.1053/j.gastro.2004.10.041 Osborn NK Ahlquist DA. Stool screening for colorectal cancer: Molecular approaches . Gastroenterology . 2005 Jan ; 128 ( 1 ): 192 – 206 . https://doi.org/10.1053/j.gastro.2004.10.041 Search in Google Scholar

Park H, Kim Y, Lim Y, Han I, Oh ES. Syndecan-2 mediates adhesion and proliferation of colon carcinoma cells. J Biol Chem. 2002 Aug 16;277(33):29730–29736. https://doi.org/10.1074/jbc.M202435200 Park H Kim Y Lim Y Han I Oh ES. Syndecan-2 mediates adhesion and proliferation of colon carcinoma cells . J Biol Chem . 2002 Aug 16 ; 277 ( 33 ): 29730 – 29736 . https://doi.org/10.1074/jbc.M202435200 Search in Google Scholar

Pawa N, Arulampalam T, Norton JD. Screening for colorectal cancer: established and emerging modalities. Nat Rev Gastroenterol Hepatol. 2011 Nov 1;8(12):711–722. https://doi.org/10.1038/nrgastro.2011.205 Pawa N Arulampalam T Norton JD. Screening for colorectal cancer: established and emerging modalities . Nat Rev Gastroenterol Hepatol . 2011 Nov 1 ; 8 ( 12 ): 711 – 722 . https://doi.org/10.1038/nrgastro.2011.205 Search in Google Scholar

R Core Team. A language and environment for statistical computing. Vienna (Austria): R Foundation for Statistical Computing; 2022 [cited 2025 Jan 15]. Available from https://www.r-project.org R Core Team A language and environment for statistical computing . Vienna (Austria) : R Foundation for Statistical Computing ; 2022 [cited 2025 Jan 15 ]. Available from https://www.r-project.org Search in Google Scholar

Rabeneck L, Paszat LF, Hilsden RJ, Saskin R, Leddin D, Grunfeld E, Wai E, Goldwasser M, Sutradhar R, Stukel TA. Bleeding and perforation after outpatient colonoscopy and their risk factors in usual clinical practice. Gastroenterology. 2008 Dec;135(6):1899–1906.e1. https://doi.org/10.1053/j.gastro.2008.08.058 Rabeneck L Paszat LF Hilsden RJ Saskin R Leddin D Grunfeld E Wai E Goldwasser M Sutradhar R Stukel TA. Bleeding and perforation after outpatient colonoscopy and their risk factors in usual clinical practice . Gastroenterology . 2008 Dec ; 135 ( 6 ): 1899 – 1906 .e1. https://doi.org/10.1053/j.gastro.2008.08.058 Search in Google Scholar

Rubinstein MR, Wang X, Liu W, Hao Y, Cai G, Han YW. Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin. Cell Host Microbe. 2013 Aug;14(2):195–206. https://doi.org/10.1016/j.chom.2013.07.012 Rubinstein MR Wang X Liu W Hao Y Cai G Han YW. Fuso-bacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin . Cell Host Microbe . 2013 Aug ; 14 ( 2 ): 195 – 206 . https://doi.org/10.1016/j.chom.2013.07.012 Search in Google Scholar

Ruf F, Schreck C, Wagner A, Grziwok S, Pagel C, Romero S, Kies-linger M, Shimono A, Peschel C, Götze KS, et al. Loss of Sfrp2 in the niche amplifies stress-induced cellular responses, and impairs the in vivo regeneration of the hematopoietic stem cell pool. Stem Cells. 2016 Sep;34(9):2381–2392. https://doi.org/10.1002/stem.2416 Ruf F Schreck C Wagner A Grziwok S Pagel C Romero S Kies-linger M Shimono A Peschel C Götze KS Loss of Sfrp2 in the niche amplifies stress-induced cellularresponses and impairs the in vivo regeneration of the hematopoietic stem cell pool . Stem Cells . 2016 Sep ; 34 ( 9 ): 2381 – 2392 . https://doi.org/10.1002/stem.2416 Search in Google Scholar

Tinmouth J, Lansdorp-Vogelaar I, Allison JE. Faecal immunochemical tests versus guaiac faecal occult blood tests: what clinicians and colorectal cancer screening programme organisers need to know. Gut. 2015 Aug;64(8):1327–1337. https://doi.org/10.1136/gutjnl-2014-308074 Tinmouth J Lansdorp-Vogelaar I Allison JE. Faecal immunochemical tests versus guaiac faecal occult blood tests: what clinicians and colorectal cancer screening programme organisers need to know . Gut . 2015 Aug ; 64 ( 8 ): 1327 – 1337 . https://doi.org/10.1136/gutjnl-2014-308074 Search in Google Scholar

Vedeld HM, Goel A, Lind GE. Epigenetic biomarkers in gastrointestinal cancers: The current state and clinical perspectives. Semin Cancer Biol. 2018 Aug;51:36–49. https://doi.org/10.1016/j.semcancer.2017.12.004 Vedeld HM Goel A Lind GE. Epigenetic biomarkers in gastrointestinal cancers: The current state and clinical perspectives . Semin Cancer Biol . 2018 Aug ; 51 : 36 – 49 . https://doi.org/10.1016/j.semcan-cer.2017.12.004 Search in Google Scholar

Wu Y, Jiao N, Zhu R, Zhang Y, Wu D, Wang AJ, Fang S, Tao L, Li Y, Cheng S, et al. Identification of microbial markers across populations in early detection of colorectal cancer. Nat Commun. 2021 May;12(1):3063. https://doi.org/10.1038/s41467-021-23265-y Wu Y Jiao N Zhu R Zhang Y Wu D Wang AJ Fang S Tao L Li Y Cheng S Identification of microbial markers across populations in early detection of colorectal cancer . Nat Commun . 2021 May ; 12 ( 1 ): 3063 . https://doi.org/10.1038/s41467-021-23265-y Search in Google Scholar

Yachida S, Mizutani S, Shiroma H, Shiba S, Nakajima T, Sakamoto T, Watanabe H, Masuda K, Nishimoto Y, Kubo M, et al. Metagenomic and metabolomic analyses reveal distinct stage-specific phenotypes of the gut microbiota in colorectal cancer. Nat Med. 2019 Jun;25(6):968–976. https://doi.org/10.1038/s41591-019-0458-7 Yachida S Mizutani S Shiroma H Shiba S Nakajima T Sakamoto T Watanabe H Masuda K Nishimoto Y Kubo M Metagenomic and metabolomic analyses reveal distinct stage-specific phenotypes of the gut microbiota in colorectal cancer . Nat Med . 2019 Jun ; 25 ( 6 ): 968 – 976 . https://doi.org/10.1038/s41591-019-0458-7 Search in Google Scholar

Zhang Q, Yu K, Li S, Zhang X, Zhao Q, Zhao X, Liu Z, Cheng H, Liu ZX, Li X. gutMEGA: A database of the human gut MEtaGenome Atlas. Brief Bioinform. 2021 May;22(3):bbaa082. https://doi.org/10.1093/bib/bbaa082 Zhang Q Yu K Li S Zhang X Zhao Q Zhao X Liu Z Cheng H Liu ZX Li X. gutMEGA: A database of the human gut MEtaGenome Atlas . Brief Bioinform . 2021 May ; 22 ( 3 ): bbaa082 . https://doi.org/10.1093/bib/bbaa082 Search in Google Scholar

Zhao G, Liu X, Liu Y, Li H, Ma Y, Li S, Zhu Y, Miao J, Xiong S, Fei S, et al. Aberrant DNA methylation of SEPT9 and SDC2 in stool specimens as an integrated biomarker for colorectal cancer early detection. Front Genet. 2020 Jun;11:643. https://doi.org/10.3389/fgene.2020.00643 Zhao G Liu X Liu Y Li H Ma Y Li S Zhu Y Miao J Xiong S Fei S Aberrant DNA methylation of SEPT9 and SDC2 in stool specimens as an integrated biomarker for colorectal cancer early detection . Front Genet . 2020 Jun ; 11 : 643 . https://doi.org/10.3389/fgene.2020.00643 Search in Google Scholar

Zhao G, Liu X, Liu Y, Ma Y, Yang J, Li H, Xiong S, Fei S, Zheng M, Zhao X. Methylated SFRP2 and SDC2 in stool specimens for colorectal cancer early detection: A cost-effective strategy for Chinese population. J Cancer. 2021 Mar;12(9):2665–2672. https://doi.org/10.7150/jca.52478 Zhao G Liu X Liu Y Ma Y Yang J Li H Xiong S Fei S Zheng M Zhao X. Methylated SFRP2 and SDC2 in stool specimens for colorectal cancer early detection: A cost-effective strategy for Chinese population . J Cancer . 2021 Mar ; 12 ( 9 ): 2665 – 2672 . https://doi.org/10.7150/jca.52478 Search in Google Scholar