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Molecular Detection of a Pathogenic Entamoeba among Symptomatic Children in Eastern Kurdistan of Iraq

Sham Jamil Abdullah
Sham Jamil Abdullah
 y 
Shahnaz Abdulkader Ali
Shahnaz Abdulkader Ali
   | 04 mar 2024
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Article Category: ORIGINAL PAPER
Publicado en línea: 04 mar 2024
Páginas: 99 - 105
Recibido: 25 sept 2023
Aceptado: 27 ene 2024
DOI: https://doi.org/10.33073/pjm-2024-010
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© 2024 Sham Jamil Abdullah et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The Lugol’s iodine wet mount of a stool sample showed cystic stage of Entamoeba species (blue arrows) applying a light microscope at a magnification of 40×.
The Lugol’s iodine wet mount of a stool sample showed cystic stage of Entamoeba species (blue arrows) applying a light microscope at a magnification of 40×.

Fig. 2.

Shows agarose gel electrophoresis for nested PCR products stained with 0.2 mg/ml ethidium bromide, using primers species specific for Entamoeba histolytica. Positive samples reveal 439 bp bands at lanes 2, 5, 8, 10–16, 19, and 21. Lane 23 represented positive control, while lane 22 represented negative control, and lane 1 is the 100 bp DNA marker.
Shows agarose gel electrophoresis for nested PCR products stained with 0.2 mg/ml ethidium bromide, using primers species specific for Entamoeba histolytica. Positive samples reveal 439 bp bands at lanes 2, 5, 8, 10–16, 19, and 21. Lane 23 represented positive control, while lane 22 represented negative control, and lane 1 is the 100 bp DNA marker.

Potential risk factors associated with Entamoeba species infection among symptomatic children.

Variable Frequency Positive Negative
Number (%)
Gender
Male 179 (55.42) 33 (18.4) 146 (81.6)
Female 144 (44.58) 25 (17.4) 119 (82.6)
p-value 0.884
Residency
Urban 164 (50.8) 22 (13.4) 142 (86.6)
Rural 159 (49.2) 36 (22.6) 123 (77.4)
p-value 0.042*
Water source
Tap water 159 (49.2) 33 (20.8) 126 (79.2)
Bottled water 164 (50.8) 25 (15.2) 139 (84.8)
p-value 0.246
Age group (years)
< 1 5.0 (1.5) 0.0 (0.0) 5.0 (100)
1–3 141 (43.7) 33 (23.4) 108 (76.6)
4–6 113 (35) 16 (14.2) 97 (85.8)
7–9 61 (18.9) 7.0 (11.5) 54 (88.5)
≥ 10 3.0 (0.9) 2.0 (66.7) 1.0 (33.3)
p-value 0.032*
Total 323 (100) 58 (18) 265 (82)

Potential risk factors associated with Entamoeba histolytica infection among microscopic positive symptomatic children.

Variable Frequency Positive Negative
Number (%)
Gender
Male 33 (18.4) 3.0 (1.7) 30 (51.57)
Female 25 (17.4) 15 (10.4) 10 (17.24)
p-value 0.001**
Residency
Urban 22 (13.4) 5.0 (3.0) 17 (29.31)
Rural 36 (22.6) 13 (8.2) 23 (39.65)
p-value 0.045*
Water source
Tap water 33 (20.8) 11 (6.9) 22 (37.93)
Bottled water 25 (15.2) 7.0 (4.3) 18 (31.03)
p-value 0.299
Age group (years)
< 1 0.0 (0.0) 0.0 (0.0) 0.0 (0.0)
1–3 33 (23.4) 14 (9.9) 19 (32.75)
4–6 16 (14.2) 1.0 (0.9) 15 (25.86)
7–9 7.0 (11.5) 2.0 (3.3) 5.0 (8.62)
≥ 10 2.0 (66.7) 1.0 (33.3) 1.0 (1.72)
p-value 0.004**
Total 58 (18) 18 (31) 40 (69)
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