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Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease

Ning Huang
Ning Huang
, 
Xin Jin
Xin Jin
, 
Jin-Tao Wen
Jin-Tao Wen
, 
Yi-Fei Zhang
Yi-Fei Zhang
, 
Xu Yang
Xu Yang
, 
Guang-Yu Wei
Guang-Yu Wei
, 
Yi-Kun Wang
Yi-Kun Wang
 y 
Min Qin
Min Qin
   | 04 mar 2024
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Article Category: ORIGINAL PAPER
Publicado en línea: 04 mar 2024
Páginas: 29 - 38
Recibido: 15 sept 2023
Aceptado: 19 dic 2023
DOI: https://doi.org/10.33073/pjm-2024-004
Palabras clave
© 2024 Ning Huang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Growth curve of Bacillus subtilis CTXW 7-6-2 and its antifungal activity against Rhizoctonia solani. A) CTXW 7-6-2 growth curve; B) a: colony morphology of the control group, b: colony morphology after confrontation with the bacteria, c: sterile fermentation broth treatment group without high-temperature treatment, d: high-temperature sterile fermented broth treatment group; e: crude lipopeptide treatment group, f: volatile compounds treatment group. The red box indicates the back of the colony.
Growth curve of Bacillus subtilis CTXW 7-6-2 and its antifungal activity against Rhizoctonia solani. A) CTXW 7-6-2 growth curve; B) a: colony morphology of the control group, b: colony morphology after confrontation with the bacteria, c: sterile fermentation broth treatment group without high-temperature treatment, d: high-temperature sterile fermented broth treatment group; e: crude lipopeptide treatment group, f: volatile compounds treatment group. The red box indicates the back of the colony.

Fig. 2.

Hypha morphology and cell ultrastructure of Rhizoctonia solani after treatment with Bacillus subtilis CTXW 7-6-2. A) Scanning electron microscopy observation of mycelium morphology; B) Ultrastructure of hyphal cells observed using transmission electron microscopy. The arrows in the figure indicate cell wall (cw), plasma membrane (pm), and mitochondria (m). a: the control hyphae, b: mycelia of the treated group
Hypha morphology and cell ultrastructure of Rhizoctonia solani after treatment with Bacillus subtilis CTXW 7-6-2. A) Scanning electron microscopy observation of mycelium morphology; B) Ultrastructure of hyphal cells observed using transmission electron microscopy. The arrows in the figure indicate cell wall (cw), plasma membrane (pm), and mitochondria (m). a: the control hyphae, b: mycelia of the treated group

Fig. 3.

Effects of A) nutrient broth medium and B) CTXW 7-6-2 fermentation broth on seedling growth of Yunyan no. 87.
Effects of A) nutrient broth medium and B) CTXW 7-6-2 fermentation broth on seedling growth of Yunyan no. 87.

Fig. 4.

Expression of genes involved in tobacco disease course induced by Bacillus subtilis CTXW 7-6-2.
Expression of genes involved in tobacco disease course induced by Bacillus subtilis CTXW 7-6-2.

Antifungal activity of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani.

Classes Control (cm) Treatment (cm) Inhibition ratio (%)
Antagonistic bacteria confront pathogenic fungi 7.11 ± 0.13 0.43 ± 0.03 94.02 ± 0.41a
Sterile fermentation broth without high-temperature treatment 3.23 ± 0.06 54.63 ± 0.89c
High-temperature processing of sterile fermentation broth 3.49 ± 0.14 50.88 ± 1.92c
100 mg/l crude lipopeptide 5.28 ± 0.01 0.23 ± 0.21 74.88 ± 4.03b
Volatile compounds 6.66 ± 0.26 0.23 ± 0.12 96.62 ± 1.77a

Effects of CTXW 7-6-2 fermentation on the growth of tobacco seedlings.

Treat CTXW 7-6-2 Nutrient broth
Leaf length (cm) 7.59 ± 0.29a 4.44 ± 0.28b
Leaf width (cm) 5.41 ± 0.31a 3.25 ± 0.23b
Leaf number (piece) 4.67 ±0.33a 4.00 ± 0.00a
Whole plant fresh weight (g) 2.01± 0.13a 0.60 ± 0.0064b
Root number 26.33 ± 0.88a 24.33 ± 0.88a
Root length (cm) 6.31 ± 0.37b 10.41 ± 0.85a
Dry weight of root (g) 0.17 ± 0.0025a 0.091 ± 0.0041b
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