Comparison of mCP and TSC Media to Enumerate Clostridium perfringens in Surface Water Samples
y | 16 dic 2023
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Article Category: ORIGINAL PAPER
Publicado en línea: 16 dic 2023
Páginas: 413 - 419
Recibido: 07 jun 2023
Aceptado: 26 sept 2023
DOI: https://doi.org/10.33073/pjm-2023-039
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© 2023 Renata Matuszewska et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
![Variability summary for RD [%].](https://sciendo-parsed.s3.eu-central-1.amazonaws.com/64727c1c215d2f6c89dc9f62/j_pjm-2023-039_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20240624T125014Z&X-Amz-SignedHeaders=host&X-Amz-Expires=18000&X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240624%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Signature=8e24d5b0424165b2a08a56e860bbc8120f7867db313abbc3eca1f76ef9cc137c)
Confirmatory tests for Clostridium perfringens according to Directive 98/83/EC.
| Feature | |
|---|---|
| Sucrose fermentation | sucrose-positive – yellow and matte colonies |
| Possess acid phosphatase | change of color of the colony from yellow to pink in ammonium hydroxide vapors |
Confirmatory tests for Clostridium perfringens according to ISO/CD 6461-2:2002.
| Feature | |
|---|---|
| Reduction of sulphites to sulphides | black or grey colonies |
| Motility | none |
| Reduction of nitrate to nitrites | orange/red-colored medium after the addition of the Nessler reagent |
| Lactose fermentation | orange or yellow-colored medium with lactose (acidification of the medium and gas formation) |
| Protease formation | gelatine liquefaction |
Comparison of the results of confirmation tests for Clostridium perfringens.
| Characteristics of |
TSC ISO/CD 6461-2:2002 | mCP Directive 98/83/EC |
|---|---|---|
| Number of all confirmed colonies | 466 | 381 |
| Typical colonies, confirmed positive (true positive) | 150 | 107 |
| Atypical colonies, confirmed positive (false negative) | 33 | 11 |
| Typical colonies, confirmed negative (false positive) | 196 | 79 |
| Atypical colonies, confirmed negative (true negative) | 87 | 184 |
| Typical colonies, non-detection error | 0.18 | 0.09 |
Comparison of performance parameters of procedures used for detection of Clostridiun perfringens in water samples.
| Performance parameters | TSC ISO/CD 6461-2:2002 | mCP Directive 98/83/EC |
|---|---|---|
| Sensitivity | 82.0% | 90.7% |
| Specificity | 30.7% | 70.0% |
| Selectivity | 32.2% | 28.1% |
| False negative rate | 27.5% | 5.6% |
| False positive rate | 56.6% | 42.5% |
| Efficiency | 50.9% | 76.4% |