Enzymes | Names | Sequences |
---|---|---|
Keratinase | KerF | 5’-ACGTCATGCTCAGATTGCAG-3’ |
KerR | 5’-GACTTCCGCGAAGAACAAAG-3’ | |
KerP | 5’-FAM-CACAATTGCTCCCGATACCT-MGB | |
Lipase | LipF | 5’-ACCCAACATTTGCTTCGTTC-3’ |
LipR | 5’-TCAATTATCAATGGTCGCGA-3’ | |
LipP | 5’-FAM-CCTTGGTGGTCGTCAAAGTT-MGB | |
Phospholipase | PhosF | 5’-AACTGGTGGATTTGCTGACC-3’ |
PhosR | 5’-CTTTACGGGTCCAAGGTGAA-3’ | |
PhosP | 5’-FAM-CCGTGTGCCATTCTACATTG-MGB | |
Actin | ActF | 5’-CTCTCCTTGTACGCCTCTGG-3’ |
ActR | 5’-TTGACAAGATGCTCCGTCAG-3’ | |
ActP | 5’-TET-GTATTGTGCTGGACTCGGGT-MGB |
Phospholipase activity number of isolates (rate of isolates) | Lipase activity number of isolates (rate of isolates) | Keratinase activity number of isolates (rate of isolates) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Mean (Pz) ±SD | very high n (%) | high n (%) | Nul n (%) | Mean (Pz) ± SD | very high n (%) | high n (%) | Mean (Pz) ± SD | very high n (%) | high n (%) | null | |
0.64 ±0.03 | 9(45) | 11 (55) | 0(0) | 0.55±0.02 | 19 (95) | 1(5) | 0.65±0.02 | 10 (50) | 6 (30) | 4(20) | |
0.76 ±0.02 | 0(0) | 7(70) | 3 (30) | 0.36 ±0.03 | 10 (100) | 0(0) | 0.69 ±0.02 | 0(0) | 2 (20) | 8 (80) | |
0.64 ±0.02 | 2 (14.29) | 12 (85.71) | 0(0) | 0.45 ±0.03 | 14 (100) | 0(0) | 0.67±0.02 | 1(7.15) | 6 (42.85) | 7(50) | |
0.67 ±0.02 | 1 (14.29) | 6 (85.71) | 0(0) | 0.54 ±0.02 | 5 (71.43) | 2 (28.57) | 0.62 ±0.03 | 1 (14.3) | 3 (42.85) | 3 (42.85) | |
0.64 ±0.02 | 2(40) | 3 (60) | 0(0) | 0.4 ±0.04 | 5(100) | 0(0) | 0.67±0.02 | 2(40) | 3 (60) | 0(0) | |
0.64 ±0.03 | 9 (42.86) | 12 (57.14) | 0(0) | 0.31 ±0.02 | 21 (100) | 0(0) | 0.68±0.02 | 3 (14.29) | 8 (38.5) | 10 (47.66) | |
Total | 0.66 ±0.02 | 23 (29.87) | 51 (66.23) | 3(3.9) | 0.48 ±0.02 | 74 (91.1) | 3 (3.9) | 0.66 ±0.02 | 17(22.08) | 28 (36.36) | 32 (41.56) |
Folliculitis (N = 12) | Neonates group (N = 20) | Pityriasis versicolor (N = 10) | Control group (N = 35) | ||
---|---|---|---|---|---|
Phospholipase activity Mean (Pz) ± SD | 0.69 ± 0.01 (5/12) | – (0/20) | 0.74 ± 0.02 (8/10) | 0.73 ± 0.02 (7/35) | |
0.67 ± 0.01 (1/12) | – (0/20) | 0.8 ± 0.02 (1/10) | 0.83 ± 0.02 (8/35) | ||
0.74 ± 0.01 (6/12) – (0/12) | 0.70 ± 0.02 (1/20) – (0/20) | 0.69 ± 0.02 (1/10) – (0/10) | 0.71 ± 0.01 (6/35) 0.78 ± 0.02 (7/35) | ||
0 (0/12) | – (0/20) | – (0/10) | 0.77 ± 0.02 (5/35) | ||
0 (0/12) | 0.66 ± 0.02 (19/20) | – (0/10) | 0.79 ± 0.01 (2/35) | ||
Total | 0.70 ± 0.01 | 0.68 ± 0.02 | 0.74 ± 0.02 | 0.77 ± 0.02 | |
Lipase activity Mean (Pz) ± SD | 0.36 ± 0.01 (5/12) | – (0/20) | 0.36 ± 0.02 (8/10) | 0.38 ± 0.02 (7/35) | |
0.37 ± 0.01 (1/12) | – (0/20) | 0.32 ± 0.02 (1/10) | 0.36 ± 0.02 (8/35) | ||
0.32 ± 0.02 (6/12) – (0/12) | 0.39 ± 0.02 (1/20) – (0/20) | 0.28 ± 0.01 (1/10) – (0/10) | 0.36 ± 0.02 (6/35) 0.48 ± 0.02 (7/35) | ||
– (0/12) | – (0/20) | – (0/10) | 0.4 ± 0.01 (5/35) | ||
– (0/12) | 0.31 ± 0.01 (19/20) | – (0/10) | 0.38 ± 0.02 (2/35) | ||
Total | 0.35 ± 0.02 | 0.35 ± 0.02 | 0.32 ± 0.02 | 0.39 ± 0.02 | |
Keratinase activity Mean (Pz) ± SD | 0.75 ± 0.02 (5/12) | – (0/20) | 0.62 ± 0.02 (8/10) | 0.85 ± 0.02 (7/35) | |
1 (1/12) | – (0/20) | 0.65 ± 0.02 (1/10) | 1 (8/35) | ||
0.82 ± 0.02 (6/12) – (0/12) | 0.53 ± 0.02 (1/20) – (0/20) | 0.65 ± 0.02 (1/10) – (0/10) | 0.96 ± 0.02 (6/35) 0.84 ± 0.02 (7/35) | ||
– (0/12) | – (0/20) | – (0/10) | 0.8 ± 0.02 (5/35) | ||
– (0/12) | 0.85 ± 0.02 (19/20) | – (0/10) | 0.79 ± 0.02 (2/35) | ||
Total | 0.86 ± 0.02 | 0.69 ± 0.02 | 0.64 ± 0.02 | 0.873 ± 0.02 |
ID | Clinical sites | RNA relative quantification | DNA relative quantification | ||||
---|---|---|---|---|---|---|---|
Keratinase | Lipase | Phospholipase | Keratinase | Lipase | Phospholipase | ||
S1 | Folliculitis | 2.11 | 1.84 | ||||
S2 | Folliculitis | 1.36 | 2.04 | 1.08 | |||
S3 | Folliculitis | 1.50 | 2.12 | ||||
S4 | Folliculitis | 0.81 | 0.32 | 0.69 | |||
S5 | Folliculitis | 1.48 | |||||
S6 | Pityriasis versicolor | 0.49 | 0.31 | 0.78 | 0.29 | 0.21 | 0.02 |
S7 | Pityriasis versicolor | 1.35 | 1.46 | 1.10 | |||
S8 | Pityriasis versicolor | 2.35 | 1.34 | ||||
S9 | Pityriasis versicolor | 1.25 | 0.77 | 1.60 | 0.24 | ||
S10 | Pityriasis versicolor | 1.96 | 1.56 | ||||
S11 | Pityriasis versicolor | 1.32 | 0.97 | 1.74 | 1.03 | ||
S12 | Pityriasis versicolor | 0.96 | 0.46 | 0.86 | |||
S13 | Pityriasis versicolor | 1.50 | 1.88 | ||||
S14 | Control group | 1.53 | 0.53 | 0.56 | 1.42 | 0.82 | 0.75 |
S15 | Control group | 1.04 | 1.19 | 0.92 | 1.05 | 0.86 | |
S16 | Control group | 0.67 | 1.36 | 0.48 | 0.50 | 0.61 | 0.32 |
S17 | Control group | 0.94 | 2.37 | 2.43 | 0.50 | 1.06 | 2.33 |
S18 | Control group | 2.36 | 0.07 | 0.48 | 1.87 | 0.26 | 0.49 |
S19 | Control group | 0.24 | 0.56 | 1.31 | 1.39 | ||
S20 | Control group | 1.50 | 1.31 | 1.18 | 0.27 |
Groups | Total | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Abs ±SD | Range | Mean Abs ±SD | Range | Mean Abs ±SD | Range | Mean Abs ±SD | Range | Mean Abs ±SD | Range | Mean Abs ±SD | Range | Mean Abs ±SD | |
Folliculitis (N= 12) | 0.321 ±0.01 | 0.155-0.41 | 0.366 ±0.01 | 0.301-0.398 | 0.339 ±0.01 | 0.317-0.594 | 0 | 0 | 0 | 0.342±0.01 | |||
Neonates (N = 20) group | 0 | 0 | 0.433 ±0.02 | 0 | 0 | 0.361 ±0.02 | 0.30-0.54 | 0.396 ±0.02 | |||||
Pityriasis versicolor (N = 10) | 0.317±0.01 | 0.16-0.604 | 0.306 ±0.02 | 0.316 ±0.02 | 0 | 0 | 0 | 0.313 ±0.02 | |||||
Control (N = 35) group | 0.328±0.01 | 0.18-0.51 | 0.338±0.02 | 0.12-0.311 | 0.255 ±0.02 | 0.12-0.311 | 0.235±0.02 | 0.137-0.361 | 0.256±0.02 | 0.167-0.331 | 0.336 ±0.02 | 0.22-0.44 | 0.291 ±0.02 |
Total | 0.322 ±0.01 | 0.155-0.604 | 0.336 ±0.02 | 0.12-0.572 | 0.336 ±0.02 | 0.12-0.594 | 0.235±0.02 | 0.137-0.362 | 0.256 ±0.03 | 0.167-0.332 | 0.349 ±0.02 | 0.22-0.54 |