Structural and Dynamic Analysis of Leaf-Associated Fungal Community of Walnut Leaves Infected by Leaf Spot Disease Based Illumina High-Throughput Sequencing Technology

Abdelfattah A, Cacciola SO, Mosca S, Zappia R, Schena L. Analysis of the fungal diversity in citrus leaves with greasy spot disease symptoms. Microb Ecol. 2017 Apr;73(3):739–749. https://doi.org/10.1007/s00248-016-0874-x Abdelfattah A Cacciola SO Mosca S Zappia R Schena L Analysis of the fungal diversity in citrus leaves with greasy spot disease symptoms Microb Ecol 2017 Apr733739 749 https://doi.org/10.1007/s00248-016-0874-x10.1007/s00248-016-0874-x27752718Search in Google Scholar

An H, Yang K. Resistance gene analogs in walnut (Juglans regia) conferring resistance to Colletotrichum gloeosporioides. Euphytica. 2014 May;197(2):175–190. https://doi.org/10.1007/s10681-013-1050-8 An H Yang K Resistance gene analogs in walnut (Juglans regia) conferring resistance to Colletotrichum gloeosporioides Euphytica 2014 May1972175 190 https://doi.org/10.1007/s10681-013-1050-810.1007/s10681-013-1050-8Search in Google Scholar

Belisario A, Forti E, Santori A, Corazza L, Balmas V, Valier A. Fusarium necrosis on Persian (English) walnut fruit. Acta Hortic. 2001 Jan;(544):389–393. https://doi.org/10.17660/ActaHortic.2001.544.51 Belisario A Forti E Santori A Corazza L Balmas V Valier A Fusarium necrosis on Persian (English) walnut fruit Acta Hortic 2001 Jan544389–393 https://doi.org/10.17660/ActaHortic.2001.544.5110.17660/ActaHortic.2001.544.51Search in Google Scholar

Belisario A, Santori A, Potente G, Fiorin A, Saphy B, Reigne JL, Pezzini C, Bortolin E, Valier A. Brown apical necrosis (BAN): A fungal disease causing fruit drop of English walnut. Acta Hortic. 2010 Apr;(861):449–452. https://doi.org/10.17660/ActaHortic.2010.861.63 Belisario A Santori A Potente G Fiorin A Saphy B Reigne JL Pezzini C Bortolin E Valier A Brown apical necrosis (BAN): A fungal disease causing fruit drop of English walnut Acta Hortic 2010 Apr861449–452 https://doi.org/10.17660/ActaHortic.2010.861.6310.17660/ActaHortic.2010.861.63Search in Google Scholar

Bennett AJ, Whipps JM. Beneficial microorganism survival on seed, roots and in rhizosphere soil following application to seed during drum priming. Biol Control. 2008 Mar;44(3):349–361. https://doi.org/10.1016/j.biocontrol.2007.11.005 Bennett AJ Whipps JM Beneficial microorganism survival on seed, roots and in rhizosphere soil following application to seed during drum priming Biol Control 2008 Mar443349 361 https://doi.org/10.1016/j.biocontrol.2007.11.00510.1016/j.biocontrol.2007.11.005Search in Google Scholar

Blakeman JP. Microbial ecology of the phylloplane. New York (USA): Academic Press Inc; 1981. Blakeman JP Microbial ecology of the phylloplane New York (USA) Academic Press Inc 1981Search in Google Scholar

Bork P, Bowler C, de Vargas C, Gorsky G, Karsenti E, Wincker P. Tara Oceans studies plankton at planetary scale. Science. 2015 May 22;348(6237):873. https://doi.org/10.1126/science.aac5605 Bork P Bowler C de Vargas C Gorsky G Karsenti E Wincker P Tara Oceans studies plankton at planetary scale Science 2015 May 223486237873 https://doi.org/10.1126/science.aac560510.1126/science.aac560525999501Search in Google Scholar

Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 2010 May;7(5):335–336. https://doi.org/10.1038/nmeth.f.303 Caporaso JG Kuczynski J Stombaugh J Bittinger K Bushman FD Costello EK Fierer N Peña AG Goodrich JK Gordon JI et al QIIME allows analysis of high-throughput community sequencing data Nat Methods 2010 May75335 336 https://doi.org/10.1038/nmeth.f.30310.1038/nmeth.f.303315657320383131Search in Google Scholar

Chen YN, Wang YF, Ye WB, Wang RJ. [Investigation and integrated management control of important walnut diseases in Longnan City, Gansu Province] (in Chinese). Biol Disaster Sci. 2021 Dec;44:15–20. Chen YN Wang YF Ye WB Wang RJ [Investigation and integrated management control of important walnut diseases in Longnan City, Gansu Province] (in Chinese) Biol Disaster Sci 2021 Dec4415 20Search in Google Scholar

Cordier T, Robin C, Capdevielle X, Fabreguettes O, Desprez-Loustau ML, Vacher C. The composition of phyllosphere fungal assemblages of European beech (Fagus sylvatica) varies significantly along an elevation gradient. New Phytol. 2012 Oct; 196(2):510–519. https://doi.org/10.1111/j.1469-8137.2012.04284.x Cordier T Robin C Capdevielle X Fabreguettes O Desprez-Loustau ML Vacher C The composition of phyllosphere fungal assemblages of European beech (Fagus sylvatica) varies significantly along an elevation gradient New Phytol 2012 Oct 1962510 519 https://doi.org/10.1111/j.1469-8137.2012.04284.x10.1111/j.1469-8137.2012.04284.x22934891Search in Google Scholar

Da Lio D, Cobo-Díaz JF, Masson C, Chalopin M, Kebe D, Giraud M, Verhaeghe A, Nodet P, Sarrocco S, Le Floch G, et al. Combined metabarcoding and multi-locus approach for genetic characterization of Colletotrichum species associated with common walnut (Juglans regia) anthracnose in France. Sci Rep. 2018 Dec; 8(1):10765. https://doi.org/10.1038/s41598-018-29027-z Da Lio D Cobo-Díaz JF Masson C Chalopin M Kebe D Giraud M Verhaeghe A Nodet P Sarrocco S Le Floch G et al Combined metabarcoding and multi-locus approach for genetic characterization of Colletotrichum species associated with common walnut (Juglans regia) anthracnose in France Sci Rep 2018 Dec 8110765 https://doi.org/10.1038/s41598-018-29027-z10.1038/s41598-018-29027-z605031530018385Search in Google Scholar

de Assis Costa OY, Tupinambá DD, Bergmann JC, Barreto CC, Quirino BF. Fungal diversity in oil palm leaves showing symptoms of Fatal Yellowing disease. PLoS One. 2018 Jan 25;13(1):e0191884. https://doi.org/10.1371/journal.pone.0191884 de Assis Costa OY Tupinambá DD Bergmann JC Barreto CC Quirino BF Fungal diversity in oil palm leaves showing symptoms of Fatal Yellowing disease PLoS One 2018 Jan 25131e0191884 https://doi.org/10.1371/journal.pone.019188410.1371/journal.pone.0191884578500329370299Search in Google Scholar

Dean R, Van Kan JAL, Pretorius ZA, Hammond-Kosack K, Di Pietro A, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, et al. The Top 10 fungal pathogens in molecular plant pathology. Mol Plant Pathol. 2012 May;13(4):414–430. https://doi.org/10.1111/j.1364-3703.2011.00783.x Dean R Van Kan JAL Pretorius ZA Hammond-Kosack K Di Pietro A Spanu PD Rudd JJ Dickman M Kahmann R Ellis J et al The Top 10 fungal pathogens in molecular plant pathology Mol Plant Pathol 2012 May134414 430 https://doi.org/10.1111/j.1364-3703.2011.00783.x10.1111/j.1364-3703.2011.00783.x663878422471698Search in Google Scholar

Donegan K, Matyac C, Seidler R, Porteous A. Evaluation of methods for sampling, recovery, and enumeration of bacteria applied to the phylloplane. Appl Environ Microbiol. 1991 Jan;57(1):51-56. https://doi.org/10.1128/aem.57.1.51-56.1991 Donegan K Matyac C Seidler R Porteous A Evaluation of methods for sampling, recovery, and enumeration of bacteria applied to the phylloplane Appl Environ Microbiol 1991 Jan57151 56 https://doi.org/10.1128/aem.57.1.51-56.199110.1128/aem.57.1.51-56.199118266316348404Search in Google Scholar

Douanla-Meli C, Langer E, Talontsi Mouafo F. Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon. Fungal Ecol. 2013 Jun;6(3):212–222. https://doi.org/10.1016/j.funeco.2013.01.004 Douanla-Meli C Langer E Talontsi Mouafo F Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon Fungal Ecol 2013 Jun63212 222 https://doi.org/10.1016/j.funeco.2013.01.00410.1016/j.funeco.2013.01.004Search in Google Scholar

Elad Y, Pertot I. Climate change impacts on plant pathogens and plant diseases. J Crop Improv. 2014 Jan 02;28(1):99–139. https://doi.org/10.1080/15427528.2014.865412 Elad Y Pertot I Climate change impacts on plant pathogens and plant diseases J Crop Improv 2014 Jan 0228199 139 https://doi.org/10.1080/15427528.2014.86541210.1080/15427528.2014.865412Search in Google Scholar

Félix CR, Andrade DA, Almeida JH, Navarro HMC, Fell JW, Landell MF. Vishniacozyma alagoana sp. nov. a tremellomycetes yeast associated with plants from dry and rainfall tropical forests. Int J Syst Evol Microbiol. 2020 May 01;70(5):3449–3454. https://doi.org/10.1099/ijsem.0.004193 Félix CR Andrade DA Almeida JH Navarro HMC Fell JW Landell MF Vishniacozyma alagoana sp nov. a tremellomycetes yeast associated with plants from dry and rainfall tropical forests. Int J Syst Evol Microbiol 2020 May 017053449 3454 https://doi.org/10.1099/ijsem.0.00419310.1099/ijsem.0.00419332375951Search in Google Scholar

Hartmann A, Rothballer M, Schmid M. Lorenz Hiltner, a pioneer in rhizosphere microbial ecology and soil bacteriology research. Plant Soil. 2008 Nov;312(1–2):7–14. https://doi.org/10.1007/s11104-007-9514-z Hartmann A Rothballer M Schmid M Lorenz Hiltner, a pioneer in rhizosphere microbial ecology and soil bacteriology research Plant Soil 2008 Nov3121–27 14 https://doi.org/10.1007/s11104-007-9514-z10.1007/s11104-007-9514-zSearch in Google Scholar

Huang X, Wang LY, Xiao QW, Pu GL, He WC. [Pathogen identification of walnut anthracnose and fungicide screening] (in Chinese). Zhongguo Nongye Daxue Xuebao. 2016 Dec;21(12):41–48. Huang X Wang LY Xiao QW Pu GL He WC [Pathogen identification of walnut anthracnose and fungicide screening] (in Chinese) Zhongguo Nongye Daxue Xuebao 2016 Dec211241 48Search in Google Scholar

Ji ZR, Zhang SZ, Shi GS, Hao HZ, Li J. [Observation on the phenophase of walnut and varieties selection for late frost avoidance] (in Chinese). Luoye Guoshu. 2021 Jan;53:13–16. Ji ZR Zhang SZ Shi GS Hao HZ Li J [Observation on the phenophase of walnut and varieties selection for late frost avoidance] (in Chinese) Luoye Guoshu 2021 Jan5313 16Search in Google Scholar

Joseph JA, Shukitt-Hale B, Willis LM. Grape juice, berries, and walnuts affect brain aging and behavior. J Nutr. 2009 Sep 01; 139(9): 1813S–1817S. https://doi.org/10.3945/jn.109.108266 Joseph JA Shukitt-Hale B Willis LM Grape juice, berries, and walnuts affect brain aging and behavior J Nutr 2009 Sep 01 1399 1813S 1817 S https://doi.org/10.3945/jn.109.10826610.3945/jn.109.10826619640963Search in Google Scholar

Karasov TL, Neumann M, Duque-Jaramillo A, Kersten S, Bezrukov I, Schröppel B, Symeonidi E, Lundberg DS, Regalado J, Shirsekar G, et al. The relationship between microbial population size and disease in the Arabidopsis thaliana phyllosphere. bioRxiv. 2020;828814. https://doi.org/10.1101/828814 Karasov TL Neumann M Duque-Jaramillo A Kersten S Bezrukov I Schröppel B Symeonidi E Lundberg DS Regalado J Shirsekar G et al The relationship between microbial population size and disease in the Arabidopsis thaliana phyllosphere bioRxiv 2020828814 https://doi.org/10.1101/82881410.1101/828814Search in Google Scholar

Lacava PT, Li WB, Araújo WL, Azevedo JL, Hartung JS. Rapid, specific and quantitative assays for the detection of the endophytic bacterium Methylobacterium mesophilicum in plants. J Microbiol Methods. 2006 Jun;65(3):535–541. https://doi.org/10.1016/j.mimet.2005.09.015 Lacava PT Li WB Araújo WL Azevedo JL Hartung JS Rapid, specific and quantitative assays for the detection of the endophytic bacterium Methylobacterium mesophilicum in plants J Microbiol Methods 2006 Jun653535 541 https://doi.org/10.1016/j.mimet.2005.09.01510.1016/j.mimet.2005.09.01516266765Search in Google Scholar

Lentendu G, Hübschmann T, Müller S, Dunker S, Buscot F, Wilhelm C. Recovery of soil unicellular eukaryotes: An efficiency and activity analysis on the single cell level. J Microbiol Methods. 2013 Dec;95(3):463–469. https://doi.org/10.1016/j.mimet.2013.05.006 Lentendu G Hübschmann T Müller S Dunker S Buscot F Wilhelm C Recovery of soil unicellular eukaryotes: An efficiency and activity analysis on the single cell level J Microbiol Methods 2013 Dec953463 469 https://doi.org/10.1016/j.mimet.2013.05.00610.1016/j.mimet.2013.05.00623680233Search in Google Scholar

Luo L, Zhang Z, Wang P, Han Y, Jin D, Su P, Tan X, Zhang D, Muhammad-Rizwan H, Lu X, et al. Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber. AMB Express. 2019 Dec;9(1):76. https://doi.org/10.1186/s13568-019-0800-y Luo L Zhang Z Wang P Han Y Jin D Su P Tan X Zhang D Muhammad-Rizwan H Lu X et al Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber AMB Express 2019 Dec9176 https://doi.org/10.1186/s13568-019-0800-y10.1186/s13568-019-0800-y653656331134393Search in Google Scholar

Lutz MC, Lopes CA, Rodriguez ME, Sosa MC, Sangorrín MP. Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear. Int J Food Microbiol. 2013 Jun;164(2–3):166–172. https://doi.org/10.1016/j.ijfoodmicro.2013.04.005 Lutz MC Lopes CA Rodriguez ME Sosa MC Sangorrín MP Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pathogens of pear Int J Food Microbiol 2013 Jun1642–3166 172 https://doi.org/10.1016/j.ijfoodmicro.2013.04.00510.1016/j.ijfoodmicro.2013.04.00523680800Search in Google Scholar

Lutz MC, Lopes CA, Sosa MC, Sangorrín MP. A new improved strategy for the selection of cold-adapted antagonist yeasts to control postharvest pear diseases. Biocontrol Sci Technol. 2012 Dec; 22(12): 1465–1483. https://doi.org/10.1080/09583157.2012.735223 Lutz MC Lopes CA Sosa MC Sangorrín MP A new improved strategy for the selection of cold-adapted antagonist yeasts to control postharvest pear diseases Biocontrol Sci Technol 2012 Dec 2212 1465 1483 https://doi.org/10.1080/09583157.2012.73522310.1080/09583157.2012.735223Search in Google Scholar

Mejía LC, Rojas EI, Maynard Z, Bael SV, Arnold AE, Hebbar P, Samuels GJ, Robbins N, Herre EA. Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biol Control. 2008 Jul; 46(1): 4–14. https://doi.org/10.1016/j.biocontrol.2008.01.012 Mejía LC Rojas EI Maynard Z Bael SV Arnold AE Hebbar P Samuels GJ Robbins N Herre EA Endophytic fungi as biocontrol agents of Theobroma cacao pathogens Biol Control 2008 Jul 461 4 14 https://doi.org/10.1016/j.biocontrol.2008.01.01210.1016/j.biocontrol.2008.01.012Search in Google Scholar

Nam HS, Yang HJ, Oh BJ, Anderson AJ, Kim YC. Biological control potential of Bacillus amyloliquefaciens KB3 isolated from the feces of Allomyrina dichotoma larvae. Plant Pathol J. 2016 Jun 01; 32(3):273–280. https://doi.org/10.5423/PPJ.NT.12.2015.0274 Nam HS Yang HJ Oh BJ Anderson AJ Kim YC Biological control potential of Bacillus amyloliquefaciens KB3 isolated from the feces of Allomyrina dichotoma larvae Plant Pathol J 2016 Jun 01 323273 280 https://doi.org/10.5423/PPJ.NT.12.2015.027410.5423/PPJ.NT.12.2015.0274489282427298603Search in Google Scholar

Qiu HP, Wang YL, Zhang Z, Mao XQ, Jiang H, Sun GC. First report of anthracnose of Digitaria sanguinalis caused by Colletotrichum hanaui in China. Plant Dis. 2010 Dec;94(12):1510. https://doi.org/10.1094/PDIS-06-10-0456 Qiu HP Wang YL Zhang Z Mao XQ Jiang H Sun GC First report of anthracnose of Digitaria sanguinalis caused by Colletotrichum hanaui in China Plant Dis 2010 Dec94121510 https://doi.org/10.1094/PDIS-06-10-045610.1094/PDIS-06-10-045630743401Search in Google Scholar

Rajendran L, Ramanathan A, Durairaj C, Samiyappan R. Endophytic Bacillus subtilis enriched with chitin offer induced systemic resistance in cotton against aphid infestation. Arch Phytopathol Pflanzenschutz. 2011 Aug;44(14):1375–1389. https://doi.org/10.1080/03235408.2010.499719 Rajendran L Ramanathan A Durairaj C Samiyappan R Endophytic Bacillus subtilis enriched with chitin offer induced systemic resistance in cotton against aphid infestation Arch Phytopathol Pflanzenschutz 2011 Aug44141375 1389 https://doi.org/10.1080/03235408.2010.49971910.1080/03235408.2010.499719Search in Google Scholar

Rastogi G, Sbodio A, Tech JJ, Suslow TV, Coaker GL, Leveau JHJ. Leaf microbiota in an agroecosystem: Spatiotemporal variation in bacterial community composition on field-grown lettuce. ISME J. 2012 Oct;6(10):1812–1822. https://doi.org/10.1038/ismej.2012.32 Rastogi G Sbodio A Tech JJ Suslow TV Coaker GL Leveau JHJ Leaf microbiota in an agroecosystem: Spatiotemporal variation in bacterial community composition on field-grown lettuce ISME J 2012 Oct6101812 1822 https://doi.org/10.1038/ismej.2012.3210.1038/ismej.2012.32344680422534606Search in Google Scholar

Rastogi G, Tech JJ, Coaker GL, Leveau JHJ. A PCR-based toolbox for the culture-independent quantification of total bacterial abundances in plant environments. J Microbiol Methods. 2010 Nov; 83(2):127–132. https://doi.org/10.1016/j.mimet.2010.08.006 Rastogi G Tech JJ Coaker GL Leveau JHJ A PCR-based toolbox for the culture-independent quantification of total bacterial abundances in plant environments J Microbiol Methods 2010 Nov 832127 132 https://doi.org/10.1016/j.mimet.2010.08.00610.1016/j.mimet.2010.08.00620816905Search in Google Scholar

Ritpitakphong U, Falquet L, Vimoltust A, Berger A, Métraux JP, L’Haridon F. The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen. New Phytol. 2016 May;210(3):1033–1043. https://doi.org/10.1111/nph.13808 Ritpitakphong U Falquet L Vimoltust A Berger A Métraux JP L’Haridon F The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen New Phytol 2016 May21031033 1043 https://doi.org/10.1111/nph.1380810.1111/nph.1380826725246Search in Google Scholar

Saremi H, Amiri ME. Evaluation of resistance to anthracnose (Marssonina juglandis) among diverse Iranian clones of walnut (Juglans regia L.). J Food Agric Environ. 2010;8(2):375–378. Saremi H Amiri ME Evaluation of resistance to anthracnose (Marssonina juglandis) among diverse Iranian clones of walnut (Juglans regia L.) J Food Agric Environ 201082375 378Search in Google Scholar

Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, et al. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009 Dec;75(23): 7537–7541. https://doi.org/10.1128/AEM.01541-09 Schloss PD Westcott SL Ryabin T Hall JR Hartmann M Hollister EB Lesniewski RA Oakley BB Parks DH Robinson CJ et al Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities Appl Environ Microbiol 2009 Dec7523 7537 7541 https://doi.org/10.1128/AEM.01541-0910.1128/AEM.01541-09278641919801464Search in Google Scholar

Scotton M, Bortolin E, Fiorin A, Belisario A. Environmental and pathogenic factors inducing brown apical necrosis on fruit of English (Persian) walnut. Phytopathology. 2015 Nov;105(11):1427–1436. https://doi.org/10.1094/PHYTO-01-15-0029-R Scotton M Bortolin E Fiorin A Belisario A Environmental and pathogenic factors inducing brown apical necrosis on fruit of English (Persian) walnut Phytopathology 2015 Nov105111427 1436 https://doi.org/10.1094/PHYTO-01-15-0029-R10.1094/PHYTO-01-15-0029-R26214123Search in Google Scholar

Sivakumar N, Sathishkumar R, Selvakumar G, Shyamkumar R, Arjunekumar K. Phyllospheric microbiomes: Diversity, ecological significance, and biotech nological applications. Sustainable Development and Biodiversity. 2020;25:113–172. https://doi.org/10.1007/978-3-030-38453-1_5 Sivakumar N Sathishkumar R Selvakumar G Shyamkumar R Arjunekumar K Phyllospheric microbiomes: Diversity, ecological significance, and biotech nological applications Sustainable Development and Biodiversity 202025113 172 https://doi.org/10.1007/978-3-030-38453-1_510.1007/978-3-030-38453-1_5Search in Google Scholar

Soriano-Hernandez AD, Madrigal-Perez DG, Galvan-Salazar HR, Arreola-Cruz A, Briseño-Gomez L, Guzmán-Esquivel J, Dobrovinskaya O, Lara-Esqueda A, Rodríguez-Sanchez IP, Baltazar-Rodriguez LM, et al. The protective effect of peanut, walnut, and almond consumption on the development of breast cancer. Gynecol Obstet Invest. 2015;80(2):89–92. https://doi.org/10.1159/000369997 Soriano-Hernandez AD Madrigal-Perez DG Galvan-Salazar HR Arreola-Cruz A Briseño-Gomez L Guzmán-Esquivel J Dobrovinskaya O Lara-Esqueda A Rodríguez-Sanchez IP Baltazar-Rodriguez LM et al The protective effect of peanut, walnut, and almond consumption on the development of breast cancer Gynecol Obstet Invest 201580289 92 https://doi.org/10.1159/00036999710.1159/00036999726183374Search in Google Scholar

Stewart CN Jr, Via LE. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Bio-Techniques. 1993 May;14(5):748–750. Stewart CN Jr Via LE A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications Bio-Techniques 1993 May145748 750Search in Google Scholar

Usyk M, Zolnik CP, Patel H, Levi MH, Burk RD. Novel ITS1 fungal primers for characterization of the mycobiome. MSphere. 2017 Dec 27;2(6):e00488-17. https://doi.org/10.1128/mSphere.00488-17 Usyk M Zolnik CP Patel H Levi MH Burk RD Novel ITS1 fungal primers for characterization of the mycobiome MSphere 2017 Dec 2726e00488 17 https://doi.org/10.1128/mSphere.00488-1710.1128/mSphere.00488-17572921829242834Search in Google Scholar

Varjas V, Lakatos T, Tóth T, Kovács C. First report of Colletotrichum godetiae causing anthracnose and twig blight on Persian walnut in Hungary. Plant Dis. 2021 Mar;105(3):702. https://doi.org/10.1094/PDIS-03-20-0607-PDN Varjas V Lakatos T Tóth T Kovács C First report of Colletotrichum godetiae causing anthracnose and twig blight on Persian walnut in Hungary Plant Dis 2021 Mar1053702 https://doi.org/10.1094/PDIS-03-20-0607-PDN10.1094/PDIS-03-20-0607-PDN33147124Search in Google Scholar

Wang QH, Fan K, Li DW, Niu SG, Hou LQ, Wu XQ. Walnut anthracnose caused by Colletotrichum siamense in China. Australas Plant Pathol. 2017 Nov;46(6):585–595. https://doi.org/10.1007/s13313-017-0525-9 Wang QH Fan K Li DW Niu SG Hou LQ Wu XQ Walnut anthracnose caused by Colletotrichum siamense in China Australas Plant Pathol 2017 Nov466585 595 https://doi.org/10.1007/s13313-017-0525-910.1007/s13313-017-0525-9Search in Google Scholar

Wang QH, Li DW, Duan CH, Liu XH, Niu SG, Hou LQ, Wu XQ. First report of walnut anthracnose caused by Colletotrichum fructicola in China. Plant Dis. 2018 Jan;102(1):247. https://doi.org/10.1094/PDIS-06-17-0921-PDN Wang QH Li DW Duan CH Liu XH Niu SG Hou LQ Wu XQ First report of walnut anthracnose caused by Colletotrichum fructicola in China Plant Dis 2018 Jan1021247 https://doi.org/10.1094/PDIS-06-17-0921-PDN10.1094/PDIS-06-17-0921-PDNSearch in Google Scholar

Wang X, Liu X, Wang R, Fa L, Chen L, Xin X, Zhang Y, Tian H, Xia M, Hou X. First report of Colletotrichum aenigma causing walnut anthracnose in China. Plant Dis. 2021 Jan;105(1):225. https://doi.org/10.1094/PDIS-07-20-1430-PDN Wang X Liu X Wang R Fa L Chen L Xin X Zhang Y Tian H Xia M Hou X First report of Colletotrichum aenigma causing walnut anthracnose in China Plant Dis 2021 Jan1051225 https://doi.org/10.1094/PDIS-07-20-1430-PDN10.1094/PDIS-07-20-1430-PDN32720888Search in Google Scholar

Yang L, Yang SY, Ma WJ, Zhou JH. [Identification of pathogen of walnut brown spot and investigation of the disease occurrence] (in Chinese). For Res. 2017 Dec;30(6):1004–1008. Yang L Yang SY Ma WJ Zhou JH [Identification of pathogen of walnut brown spot and investigation of the disease occurrence] (in Chinese) For Res 2017 Dec3061004 1008Search in Google Scholar

Yashiro E, Spear RN, McManus PS. Culture-dependent and culture-independent assessment of bacteria in the apple phyllosphere. J Appl Microbiol. 2011 May;110(5):1284–1296. https://doi.org/10.1111/j.1365-2672.2011.04975.x Yashiro E Spear RN McManus PS Culture-dependent and culture-independent assessment of bacteria in the apple phyllosphere J Appl Microbiol 2011 May11051284 1296 https://doi.org/10.1111/j.1365-2672.2011.04975.x10.1111/j.1365-2672.2011.04975.x21332895Search in Google Scholar

Yuan Y, Feng H, Wang L, Li Z, Shi Y, Zhao L, Feng Z, Zhu H. Potential of endophytic fungi isolated from cotton roots for biological control against Verticillium wilt disease. PLoS One. 2017 Jan 20;12(1):e0170557. https://doi.org/10.1371/journal.pone.0170557 Yuan Y Feng H Wang L Li Z Shi Y Zhao L Feng Z Zhu H Potential of endophytic fungi isolated from cotton roots for biological control against Verticillium wilt disease PLoS One 2017 Jan 20121e0170557 https://doi.org/10.1371/journal.pone.017055710.1371/journal.pone.0170557524920828107448Search in Google Scholar

Zhang Z, Luo L, Tan X, Kong X, Yang J, Wang D, Zhang D, Jin D, Liu Y. Pumpkin powdery mildew disease severity influences the fungal diversity of the phyllosphere. PeerJ. 2018 Apr 02;6:e4559. https://doi.org/10.7717/peerj.4559 Zhang Z Luo L Tan X Kong X Yang J Wang D Zhang D Jin D Liu Y Pumpkin powdery mildew disease severity influences the fungal diversity of the phyllosphere PeerJ 2018 Apr 026e4559 https://doi.org/10.7717/peerj.455910.7717/peerj.4559588598729629242Search in Google Scholar

Zhu YZ, Liao WJ, Zou DX, Wu YJ, Zhou Y. First report of leaf spot disease on walnut caused by Colletotrichum fioriniae in China. Plant Dis. 2015 Feb;99(2):289. https://doi.org/10.1094/PDIS-09-14-0938-PDN Zhu YZ Liao WJ Zou DX Wu YJ Zhou Y First report of leaf spot disease on walnut caused by Colletotrichum fioriniae in China Plant Dis 2015 Feb992289 https://doi.org/10.1094/PDIS-09-14-0938-PDN10.1094/PDIS-09-14-0938-PDN30699588Search in Google Scholar