Evaluation of three common methods of bulk lipid quantification in soft tissues of marine benthic invertebrates

Figure 1

Time consumption (min) of analyses of 20 samples (20S), preparation of SSS and OR using three analytical methods of quantitative bulk lipid determination

Analytical method	Time consumption
	20S	SSS	OR	Total
Gravimetric	45	0	0	45
Marsha and Weinstein	75	20	0	95
Frings and Dunn	120	35	20	175

Results of one-way ANOVA of differences in bulk lipid content amongst macrobenthic invertebrate species analysed by different analytical methods (a) and amongst three analytical methods for the same species (b)_

a)
Analytical method	F	p	df
Gravimetric	54.380	<0.001	2
Marsh and Weinstein	670.220	<0.001	2
Frings and Dunn	142.186	<0.001	2

Mean lipid content (%) in soft tissues of marine benthic invertebrates from the southern Baltic Sea determined by three analytical methods of quantitative bulk lipid determination following the Bligh and Dyer extraction_

Analytical method	Species	Mean	SD	SE	n
Gravimetric	Mytilus trossulus	14.85	2.95	0.66	20
	Crangon crangon	6.37	2.08	0.49	18
	Hediste diversicolor	10.81	2.36	0.53	20
Marsh and Weinstein	Mytilus trossulus	13.79	0.55	0.13	19
	Crangon crangon	7.41	0.34	0.08	18
	Hediste diversicolor	10.44	0.65	0.15	19
Frings and Dunn	Mytilus trossulus	13.27	1.20	0.27	20
	Crangon crangon	7.38	1.04	0.23	20
	Hediste diversicolor	10.64	1.08	0.24	20

Precision (CD, %), limit of detection (LOD, %) and recovery (R, %) for analyses of bulk lipid content in marine benthic invertebrates using the gravimetric, Marsh and Weinstein and Frings and Dunn methods

Analytical method	Species	CD	LOD	R
Gravimetric	Mytilus trossulus	19.85	1.12	89.06
	Crangon crangon	32.68	0.98	101.67
	Hediste diversicolor	21.88	0.11	97.30
Marsha and Weinstein	Mytilus trossulus	4.30	0.01	102.50
	Crangon crangon	4.56	0.03	95.17
	Hediste diversicolor	6.21	0.01	100.79
Frings and Dunn	Mytilus trossulus	9.05	0.27	95.16
	Crangon crangon	14.05	0.29	94.85
	Hediste diversicolor	10.12	0.32	98.16