<abstract xmlns="http://www.w3.org/1999/xhtml"><p> High frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon ‘Day Light’ has been developed through a new medium, Teixeira Cymbidium (TC) medium. Two new TC media containing variable amounts of macroand micronutrients and other additives, inspired by Winarto and Teixeira (WT) medium for Anthurium and Murashige and Skoog (MS) basal medium were used to induce PLBs and callus. Control medium was research- and industry-standard Vacin and Went (VW) medium. The first TC medium, TCPLB, could induce significantly more PLBs than on VW while high levels of macronutrients in the second TC medium, TCCALLUS, and MS were required to induce callus. All PLB induction media contained 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar while callusinduction media were identical, except that KIN was substituted by thidiazuron (TDZ). Basal medium had a significant effect on PLB and callus formation. This protocol could be used to induce PLBs and callus from other Cymbidium species or cultivars.</p></abstract>

High frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon ‘Day Light’ has been developed through a new medium, Teixeira Cymbidium (TC) medium. Two new TC media containing variable amounts of macroand micronutrients and other additives, inspired by Winarto and Teixeira (WT) medium for Anthurium and Murashige and Skoog (MS) basal medium were used to induce PLBs and callus. Control medium was research- and industry-standard Vacin and Went (VW) medium. The first TC medium, TCPLB, could induce significantly more PLBs than on VW while high levels of macronutrients in the second TC medium, TCCALLUS, and MS were required to induce callus. All PLB induction media contained 0.1 mg/l α-naphthaleneacetic acid (NAA) and 0.1 mg/l kinetin (KIN), 2 g/l tryptone and 20 g/l sucrose, and solidified with 8 g/l Bacto agar while callusinduction media were identical, except that KIN was substituted by thidiazuron (TDZ). Basal medium had a significant effect on PLB and callus formation. This protocol could be used to induce PLBs and callus from other Cymbidium species or cultivars.

New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

Faculty of Agriculture and Graduate School of Agriculture Kagawa University, Miki-cho, Kagawa, 761-0795, JAPAN

Journal of Fruit and Ornamental Plant Research

Short communication

High frequency protocorm-like body (PLB) production from hybrid Cymbidium Twilight Moon ‘Day Light’ has been developed through a new medium, Teixeira Cymbidium...

New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

Publicado en línea: 30 may 2013

Páginas: 127 - 133

DOI: https://doi.org/10.2478/v10290-012-0022-8

Palabras clave
basal medium, PLBs and callus, Teixeira Cymbidium (TC) medium, Winarto and Teixeira (WT) medium

This content is open access.

New Basal Media for Protocorm-Like Body and Callus Induction of Hybrid Cymbidium

Publicado en línea: 30 may 2013

Páginas: 127 - 133

DOI: https://doi.org/10.2478/v10290-012-0022-8

Palabras clavebasal medium, PLBs and callus, Teixeira Cymbidium (TC) medium, Winarto and Teixeira (WT) medium

This content is open access.

Palabras clave
basal medium, PLBs and callus, Teixeira Cymbidium (TC) medium, Winarto and Teixeira (WT) medium