<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> This article presents the essential stages leading to development of a modified method for <italic>Hypoderma bovis </italic>protein transfer, and estimation of the usefulness of semi-quantitative densitometric analysis of western-blottig results. The principal of the method was to incubate the gel obtained with the native electrophoresis method in buffer containing SDS prior to transfer of the separated proteins of L1 <italic>H. bovis </italic>onto nitrocellulose membrane. In two experiments, the authors estimated the efficacy of the new method and the possibility of using this method for semi-quantitative densitometric investigations.</p>
</abstract>

This article presents the essential stages leading to development of a modified method for Hypoderma bovis protein transfer, and estimation of the usefulness of semi-quantitative densitometric analysis of western-blottig results. The principal of the method was to incubate the gel obtained with the native electrophoresis method in buffer containing SDS prior to transfer of the separated proteins of L1 H. bovis onto nitrocellulose membrane. In two experiments, the authors estimated the efficacy of the new method and the possibility of using this method for semi-quantitative densitometric investigations.

Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

Department of Parasitology and Invasive Diseases

Journal of Veterinary Research

This article presents the essential stages leading to development of a modified method for Hypoderma bovis protein transfer, and estimation of the usefulness...

Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

Publicado en línea: 17 ene 2013

Páginas: 547 - 552

DOI: https://doi.org/10.2478/v10213-012-0096-z

Palabras clave
Hypoderma bovis, protein, gel transfer, western-blotting, densitometric analysis

This content is open access.

Modified Method of Hypoderma Bovis Proteins Transfer Obtained from Gel by Native Electrophoresis onto Nitrocellulose Membrane

Publicado en línea: 17 ene 2013

Páginas: 547 - 552

DOI: https://doi.org/10.2478/v10213-012-0096-z

Palabras claveHypoderma bovis, protein, gel transfer, western-blotting, densitometric analysis

This content is open access.

Palabras clave
Hypoderma bovis, protein, gel transfer, western-blotting, densitometric analysis