The aim of the study was to determine Mycoplasma mycoides subsp. mycoides small colony variant (MmmSC) and Mycoplasma agalactiae antibodies in ruminants from different provinces/regions of Poland. Eight hundred and ten bovine serum samples were examined for MmmSC antibodies by the use of competitive ELISA (c-ELISA) and complement fixation test (CFT). ELISA was also used for M. agalactiae antibody detection in 951 serum samples of sheep and goats. The first screening serological examination of MmmSC antibodies using c-ELISA revealed two (0.25%) positive and 135 (16.92%) doubtful results. The second examination revealed only 52 doubtful results, whereas the rest samples were negative. To compare, the final confirmatory examination by CFT gave 100% of seronegative results. The examination performed in small ruminants demonstrated only one doubtful result, which was finally defined as negative following the second ELISA, whereas the remaining samples were negative. To conclude, the present serological study showed the lack of infections in Polish domestic ruminant caused by two mycoplasmas.
