<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>We investigated the effects of <italic>N'</italic>-nitrosodimethylamine (NDMA) induced toxicity on red blood cell rheology in male rats and identified bands in proteomic profiles of brain which can be used as novel markers. Polyacrylamide gel electrophoresis (PAGE) profiles exhibited constitutive as well as induced expression of the polypeptides. Remarkably, the molecular weight range of the polypeptides (8-150 kDa) corresponded to that of the family of heat shock proteins. Our results revealed significant changes in blood parameters and showed the presence of acanthocytes, tear drop cells, spicules and cobot rings in the treated categories. Lactate dehydrogenase and esterase zymograms displayed a shift to anaerobic metabolism generating hypoxia-like conditions. This study strongly suggests that NDMA treatment causes acute toxicity leading to cell membrane destruction and alters protein profiles in rats. It is therefore recommended that caution should be exercised in using NDMA to avoid risks, and if at all necessary strategies should be designed to combat such conditions.</p></abstract>

We investigated the effects of N'-nitrosodimethylamine (NDMA) induced toxicity on red blood cell rheology in male rats and identified bands in proteomic profiles of brain which can be used as novel markers. Polyacrylamide gel electrophoresis (PAGE) profiles exhibited constitutive as well as induced expression of the polypeptides. Remarkably, the molecular weight range of the polypeptides (8-150 kDa) corresponded to that of the family of heat shock proteins. Our results revealed significant changes in blood parameters and showed the presence of acanthocytes, tear drop cells, spicules and cobot rings in the treated categories. Lactate dehydrogenase and esterase zymograms displayed a shift to anaerobic metabolism generating hypoxia-like conditions. This study strongly suggests that NDMA treatment causes acute toxicity leading to cell membrane destruction and alters protein profiles in rats. It is therefore recommended that caution should be exercised in using NDMA to avoid risks, and if at all necessary strategies should be designed to combat such conditions.

Effect of <italic>N'</italic>-nitrosodimethylamine on red blood cell rheology and proteomic profiles of brain in male albino rats

Biochemical and Clinical Genetics Research Laboratory, Section of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh, India

Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India

Interdisciplinary Toxicology

Effect of -nitrosodimethylamine on red blood cell rheology and proteomic profiles of brain in male albino rats

We investigated the effects of N'-nitrosodimethylamine (NDMA) induced toxicity on red blood cell rheology in male rats and identified bands in proteomic...

Effect of N'-nitrosodimethylamine on red blood cell rheology and proteomic profiles of brain in male albino rats

Publicado en línea: 24 oct 2011

Páginas: 125 - 131

DOI: https://doi.org/10.2478/v10102-011-0020-z

Palabras clave
hepatic fibrosis, hypoxia, -nitrosodimethylamine (NDMA), proteomic profiles, RBC rheology

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