<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Theoretical and experimental bases of TILLING and FOX-hunting, new tools for precise identification of genes in functional studies are reviewed. TILLING (Targeting Induced Local Lesions IN Genomes) is a technique combining chemical mutagenesis with its sensitive and precise detection. The method involves PCR amplification of DNA samples pooled following extraction from a collection of chemically-treated organisms and a subsequent screening for mutations using Cel1 endonuclease, detecting mismatches in heteroduplexes [52]. FOX-hunting (Full-length cDNA Over-eXpressing gene hunting system) is a new method of plant gene overexpression, which enables a quick gene isolation and sequencing, paralelly with functional studies.</p></abstract>

Theoretical and experimental bases of TILLING and FOX-hunting, new tools for precise identification of genes in functional studies are reviewed. TILLING (Targeting Induced Local Lesions IN Genomes) is a technique combining chemical mutagenesis with its sensitive and precise detection. The method involves PCR amplification of DNA samples pooled following extraction from a collection of chemically-treated organisms and a subsequent screening for mutations using Cel1 endonuclease, detecting mismatches in heteroduplexes [52]. FOX-hunting (Full-length cDNA Over-eXpressing gene hunting system) is a new method of plant gene overexpression, which enables a quick gene isolation and sequencing, paralelly with functional studies.

Tilling and Fox-Hunting: New Methods for Functional Analysis of Genes

Department of Plant Biochemistry and Physiology, Plant Breeding and Acclimatization Institute, 05-870 Blonie

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Tilling and Fox-Hunting: New Methods for Functional Analysis of Genes"}

Theoretical and experimental bases of TILLING and FOX-hunting, new tools for precise identification of genes in functional studies are reviewed. TILLING...

Tilling and Fox-Hunting: New Methods for Functional Analysis of Genes

Publicado en línea: 15 oct 2014

Páginas: 1 - 16

DOI: https://doi.org/10.2478/v10052-011-0001-6

© by Krystyna Rybka

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.