<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><bold>Background.</bold> The aim of the study was to investigate the suppressive effects of pSilencer2.1-U6-siRNA-<italic>stat3</italic> recombinant plasmids on the growth of ovarian cancer <italic>in vitro</italic>.<bold>Material and methods.</bold> Three pairs of DNA template (<italic>stat3</italic>-1, <italic>stat3</italic>-2, <italic>stat3</italic>-3) specific for different target sites on <italic>stat3</italic> mRNA were synthesized to reconstruct pSilencer2.1-U6-siRNA-<italic>stat3</italic>s, which were transfected into SKOV3 cells. The expressions of STAT3, BcL-2, cyclin D1 and C-myc in these cells were detected by Western blot and Northern blot. The cell cycle and the growth were determined by flow cytometry (FCM) and MTT assay, respectively. Cell apoptosis was determined by TUNEL staining.<bold>Results.</bold> Of the three siRNAs, only siRNA targeting <italic>stat3</italic>-3 markedly suppressed the protein expression of <italic>stat3</italic> in SKOV3 cells; MTT assay and FCM showed that transfection of <italic>stat3</italic>-3 siRNA could significantly suppress the growth of SKOV3 cells and arrest the cell cycle <italic>in vitro</italic>. TUNEL staining also showed massive apoptosis in SKOV3 cells transfected with <italic>stat3</italic>-3 siRNA.<bold>Conclusions.</bold> pSilencer2.1-U6-siRNA-<italic>stat3</italic>-3 can significantly inhibit the STAT3 expression in human ovarian cancer cells resulting in the inhibition of the cancer growth and the increase of apoptosis of cancer cells.</abstract>

Background. The aim of the study was to investigate the suppressive effects of pSilencer2.1-U6-siRNA-stat3 recombinant plasmids on the growth of ovarian cancer in vitro.Material and methods. Three pairs of DNA template (stat3-1, stat3-2, stat3-3) specific for different target sites on stat3 mRNA were synthesized to reconstruct pSilencer2.1-U6-siRNA-stat3s, which were transfected into SKOV3 cells. The expressions of STAT3, BcL-2, cyclin D1 and C-myc in these cells were detected by Western blot and Northern blot. The cell cycle and the growth were determined by flow cytometry (FCM) and MTT assay, respectively. Cell apoptosis was determined by TUNEL staining.Results. Of the three siRNAs, only siRNA targeting stat3-3 markedly suppressed the protein expression of stat3 in SKOV3 cells; MTT assay and FCM showed that transfection of stat3-3 siRNA could significantly suppress the growth of SKOV3 cells and arrest the cell cycle in vitro. TUNEL staining also showed massive apoptosis in SKOV3 cells transfected with stat3-3 siRNA.Conclusions. pSilencer2.1-U6-siRNA-stat3-3 can significantly inhibit the STAT3 expression in human ovarian cancer cells resulting in the inhibition of the cancer growth and the increase of apoptosis of cancer cells.

Knockdown of <italic>stat3</italic> expression by RNAi inhibits <italic>in vitro</italic> growth of human ovarian cancer

Second Clinical Hospital of Jilin University, Changchun, China

Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun, China

Radiology and Oncology

Knockdown of  expression by RNAi inhibits  growth of human ovarian cancer

Background. The aim of the study was to investigate the suppressive effects of pSilencer2.1-U6-siRNA-stat3 recombinant plasmids on the growth of ovarian cancer...

Knockdown of stat3 expression by RNAi inhibits in vitro growth of human ovarian cancer

Publicado en línea: 28 abr 2011

Páginas: 196 - 203

DOI: https://doi.org/10.2478/v10019-011-0013-8

Palabras clave
RNA interference, SKOV3 cells, ovarian cancer, apoptosis

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