<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Background</bold>. Regulatory T (Treg) cell plays a key role in autoimmune diseases. We evaluated the regulatory function and frequency of Treg cells and secreted IL-10, IL-35 concentration in Crohn’s disease (CD).<bold>Methods</bold>. Twenty-three patients with CD and 25 healthy controls (HC) were included in this study. We analysed the alteration of Tregs frequency using flow cytometry for CD4, CD25, CD127 and FoxP3 markers. Surface expression of CD4, CD25 and CD127 markers were used for isolation of relatively pure Treg cells. Suppressive activity of Tregs was determined by measuring their ability to inhibit the proliferation of T responder (Tres) cells. In addition, the amounts of IL-10 and IL-35 cytokines in co-culture supernatants were measured by ELISA assay after stimulation with anti-CD2/CD3/CD28.<bold>Results</bold>. CD patients had significantly lower frequency of CD4+ CD25+ CD127low FoxP3+ Treg cells in comparison with controls (2.17 ± 1.04 <italic>vs</italic>. 2.83 ± 1.07, p = 0.0352). Additionally, Treg cells mediated suppression was not significantly different in CD patients compared to controls. There was a significant difference in IL-10 secretion in response to anti-CD2/CD3/CD28 stimulation compared with HC (p = 0.0074).<bold>Conclusion</bold>. The frequency of CD4+ CD25+ CD127low FoxP3+ Tregs decreased in active stage of CD but there was no impaired suppressive function of CD4+ CD25+ CD127low FoxP3+ Treg cells. We suggest that an alteration in the balance of Tregs and T effectors may contribute to pathogenesis of CD.</abstract>

Background. Regulatory T (Treg) cell plays a key role in autoimmune diseases. We evaluated the regulatory function and frequency of Treg cells and secreted IL-10, IL-35 concentration in Crohn’s disease (CD).Methods. Twenty-three patients with CD and 25 healthy controls (HC) were included in this study. We analysed the alteration of Tregs frequency using flow cytometry for CD4, CD25, CD127 and FoxP3 markers. Surface expression of CD4, CD25 and CD127 markers were used for isolation of relatively pure Treg cells. Suppressive activity of Tregs was determined by measuring their ability to inhibit the proliferation of T responder (Tres) cells. In addition, the amounts of IL-10 and IL-35 cytokines in co-culture supernatants were measured by ELISA assay after stimulation with anti-CD2/CD3/CD28.Results. CD patients had significantly lower frequency of CD4+ CD25+ CD127low FoxP3+ Treg cells in comparison with controls (2.17 ± 1.04 vs. 2.83 ± 1.07, p = 0.0352). Additionally, Treg cells mediated suppression was not significantly different in CD patients compared to controls. There was a significant difference in IL-10 secretion in response to anti-CD2/CD3/CD28 stimulation compared with HC (p = 0.0074).Conclusion. The frequency of CD4+ CD25+ CD127low FoxP3+ Tregs decreased in active stage of CD but there was no impaired suppressive function of CD4+ CD25+ CD127low FoxP3+ Treg cells. We suggest that an alteration in the balance of Tregs and T effectors may contribute to pathogenesis of CD.

CD4+CD25+CD127low FoxP3+ regulatory T cells in Crohn’s disease

Mazandaran University of Medical Sciences

Infectious Diseases and Tropical Medicine Research Center, Health Research Institute

Department of Immunology, School of Medicine

Romanian Journal of Internal Medicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

CD4CD25CD127 FoxP3 regulatory T cells in Crohn’s disease

Background. Regulatory T (Treg) cell plays a key role in autoimmune diseases. We evaluated the regulatory function and frequency of Treg cells and secreted...

CD4⁺CD25⁺CD127^low FoxP3⁺ regulatory T cells in Crohn’s disease

Publicado en línea: 29 ago 2018

Páginas: 158 - 166

Recibido: 13 ene 2018

DOI: https://doi.org/10.2478/rjim-2018-0006

Palabras clave
Crohn’s disease, Regulatory T cells, IL-10, IL-35

© 2018 Ali Khalili et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

CD4+CD25+CD127low FoxP3+ regulatory T cells in Crohn’s disease

Publicado en línea: 29 ago 2018

Páginas: 158 - 166

Recibido: 13 ene 2018

DOI: https://doi.org/10.2478/rjim-2018-0006

Palabras claveCrohn’s disease, Regulatory T cells, IL-10, IL-35

© 2018 Ali Khalili et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

CD4⁺CD25⁺CD127^low FoxP3⁺ regulatory T cells in Crohn’s disease

Palabras clave
Crohn’s disease, Regulatory T cells, IL-10, IL-35