Evaluation of the training program for p16/ Ki-67 dual immunocytochemical staining interpretation for laboratory staff without experience in cervical cytology and immunocytochemistry
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26 mar 2020
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Categoría del artículo: Research Article
Publicado en línea: 26 mar 2020
Páginas: 201 - 208
Recibido: 22 ene 2020
Aceptado: 04 mar 2020
DOI: https://doi.org/10.2478/raon-2020-0018
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© 2020 Veronika Kloboves Prevodnik, Ziva Pohar Marinsek, Janja Zalar, Hermina Rozina, Nika Kotnik, Tine Jerman, Jerneja Varl, Urska Ivanus, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
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Individual comparison of p16/Ki-67 agreement and performance between students, senior technologist and reference
| Training | Reviewers | OPA | McNemar’s test p | κ (cohen) (95% CI) |
|---|---|---|---|---|
| S1/Reference | 81.4% | 0.000 | 0.512 (CI: 0.329–0,696) | |
| S2/Reference | 81.4% | 0.006 | 0.527 (CI: 0.349–0,705) | |
| Initial training | SC (S1)/ Reference | 94.1% | 0.131 | 0.859 (CI: 0.758–0.960) |
| (N = 118) | SC (S2)/ Reference | 94.1% | 0.131 | 0.859 (CI: 0.758–0.960) |
| SC (S1)/SC (S2) | 100.0% | / | 1.000 (CI: /) | |
| S1/Reference | 78.3% | 0.000 | 0.556 (Cl: 0.472–0.641) | |
| S2/Reference | 87.2% | 0.775 | 0.713 (Cl: 0.638–0.788) | |
| Addtional (N = 383) | SC (S1)/Reference | 86.2% | 0.006 | 0.700 (Cl: 0.625–0,775) |
| SC (S2)/Reference | 86.4% | 0.004 | 0.707 (Cl: 0.632–0.781) | |
| SC (S1)/SC (S2) | 98.7% | 1.000 | 0.973 (CI: 0.949–0.996) |
p16/Ki-67 study results and CIN2+ outcome for students, senior cytotechnologist and reference
| Reviewer | Categories of p16/Ki67 dual staining result | Initial training (N = 118) | Additional training (N = 383) | ||
|---|---|---|---|---|---|
| p16/Ki67 dual staining result N, (%) | CIN2+ outcomes N (PV, %)* | p16/Ki67 dual staining result (N, %) | CIN2+ outcomes N (PV, %)* | ||
| positive | 91 (77.1) | 65 (71.4) | 171 (44.6) | 65 (38.0) | |
| S1 | suspicious | 7 (5.9) | 2 (28.6) | 7 (1.8) | 2 (28.6) |
| negative | 20 (16.9) | 2 (10.0) | 205 (53.5) | 10 (4.9) | |
| unsatisfactory | 0 (0.0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 92 (78.0) | 66 (71.7) | 129 (33.7) | 64 (49.6) | |
| S2 | suspicious | 2 (1.7) | 0 (0.0) | 1 (0.3) | 1 (100.0) |
| negative | 24 (20.3) | 3 (12.5) | 253 (66.1) | 12 (4.7) | |
| unsatisfactory | 0 (0) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 83 (70.3) | 65 (78.3) | 143 (37.9) | 65 (45.5) | |
| suspicious | 2 (1.7) | 2 (100.0) | 5 (1.3) | 0 (0.0) | |
| SC (S1) | negative | 32 (27.1) | 2 (6.3) | 235 (61.4) | 12 (5.1) |
| unsatisfactory | 1 (0.8) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 83 (70.3) | 65 (78.3) | 145 (37.9) | 64 (44.1) | |
| suspicious | 2 (1.7) | 2 (100.0) | 4 (1.0) | 0 (0.0) | |
| SC (S2) | negative | 32 (27.1) | 2 (6.3) | 234 (61.1) | 13 (5.6) |
| unsatisfactory | 1 (0.8) | 0 (0.0) | 0 (0.0) | 0 (0.0) | |
| positive | 78 (66.1) | 64 (82.1) | 114 (29.8) | 64 (56.1) | |
| Reference | suspicious | 2 (1.7) | 0 (0) | 13 (3.4) | 3 (23.1) |
| negative | 38 (32.2) | 5 (13.2) | 255 (66.6) | 10 (3.9) | |
| unsatisfactory | 0 (0.0) | 0 (0.0) | 1 (0.3) | 0 (0.0) | |